Development and analysis of cancer-specific monoclonal antibodies for glycoproteins

• Project/Area Number: 19K07705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 50020:Tumor diagnostics and therapeutics-related
• Research Institution: Tohoku University
• Principal Investigator: KATO Yukinari 東北大学, 医学系研究科, 教授 (00571811)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: モノクローナル抗体 / がん特異的抗体 / FACS / 免疫染色 / ADCC / CDC / 抗腫瘍効果 / コアフコース欠損抗体 / 抗体 / エピトープ / エピトープマッピング / CasMab法 / 抗体医薬 / がん / がん細胞 / スクリーニング / ハイブリドーマ / クローン / がん特異的 / CasMab / 正常細胞 / フローサイトメトリー / 免疫 / 膜タンパク質 / 糖鎖 / 糖ペプチド / 糖タンパク質

Outline of Research at the Start

モノクローナル抗体は実験的ツールや診断薬としてだけでなく、様々ながん治療に応用されている。一方、がん細胞に特異的な抗体を樹立しなければ、常に正常組織への毒性が懸念される。しかし、がん細胞だけに高発現している分子は限られているため、新規の標的分子を発見するのは困難である。
本課題においては、申請者が開発したがん特異的抗体作製法や糖鎖改変技術を駆使し、種々の糖タンパク質に対するがん特異的抗体を作製し、その有用性の証明および最適化を目指す。

Outline of Final Research Achievements

EGFR contributes to tumor malignancy via gene amplification and protein overexpression. Previously, we developed an anti-human EGFR (hEGFR) monoclonal antibody, namely, EMab-134, which detects hEGFR and dog EGFR (dEGFR) with high sensitivity and specificity. In this study, we produced a defucosylated mouse-dog chimeric anti-EGFR monoclonal antibody, namely, E134Bf. In vitro analysis revealed that E134Bf highly exerted antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity against a canine osteosarcoma cell line (D-17) and a canine fibroblastic cell line (A-72), both of which express endogenous dEGFR. Moreover, in vivo administration of E134Bf significantly suppressed the development of D-17 and A-72 compared with the control dog IgG in mouse xenografts. These results indicate that E134Bf exerts antitumor effects against dEGFR-expressing canine cancers and could be valuable as part of an antibody treatment regimen for dogs.

Academic Significance and Societal Importance of the Research Achievements

EGFRに対する抗体医薬は複数開発されているが、その副作用により患者さんのQOLが良くない。我々が開発したCasMab法は、複数のタンパク質に対してがん特異的抗体の作製に成功してきた。今回、EGFRに対する抗体作製を実施した結果、EMab-134という特異的抗体の作製に成功した。また、イヌ腫瘍のマウス移植片モデルにおいて、EMab-134のイヌ化抗体よる高い抗腫瘍効果が得られた。今後、獣医師と共同で、イヌ腫瘍の治療法の開発および臨床試験を行う。さらに、ヒトEGFR高発現がんに対する治療法への応用へと発展させる。

Research Products

• [Journal Article] Epitope Mapping of an Anti-CD20 Monoclonal Antibody (C₂₀Mab-60) Using Enzyme-Linked Immunosorbent Assay (2021)
  • Author(s): Takei Junko、Suzuki Hiroyuki、Asano Teizo、Li Guanjie、Saito Masaki、Kaneko Mika K.、Kato Yukinari
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 40 Issue: 6 Pages: 250-254
  • DOI: 10.1089/mab.2021.0042
  • Peer Reviewed
• [Journal Article] Epitope Mapping of an Anti-Human CCR9 Monoclonal Antibody (C9Mab-1) using Enzyme-Linked Immunosorbent Assay (2021)
  • Author(s): Takei J, Asano T, Li G, Saito M, Suzuki H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 6 Pages: 239-242
  • DOI: 10.1089/mab.2021.0037
  • Peer Reviewed
• [Journal Article] Epitope Mapping of an Anti-HER2 Monoclonal Antibody (H2Mab-181) using Enzyme-Linked Immunosorbent Assay (2021)
  • Author(s): Asano T, Takei J, Suzuki H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 6 Pages: 255-260
  • DOI: 10.1089/mab.2021.0029
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of an Anti-CD20 Monoclonal Antibody (C20Mab-60) using the HisMAP Method (2021)
  • Author(s): Asano T, Takei J, Furusawa Y, Saito M, Suzuki H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 6 Pages: 243-249
  • DOI: 10.1089/mab.2021.0035
  • Peer Reviewed / Open Access
• [Journal Article] Defucosylated Mouse-Dog Chimeric Anti-EGFR Antibody Exerts Antitumor Activities in Mouse Xenograft Models of Canine Tumors (2021)
  • Author(s): Li G, Ohishi T, Kaneko MK, Takei J, Mizuno T, Kawada M, Saito M, Suzuki H, Kato Y.
  • Journal Title: Cells Volume: 10 Issue: 12 Pages: 3599-3599
  • DOI: 10.3390/cells10123599
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of the Anti-CD44 Monoclonal Antibody (C₄₄Mab-46) Using Alanine-Scanning Mutagenesis and Surface Plasmon Resonance (2021)
  • Author(s): Takei Junko、Asano Teizo、Suzuki Hiroyuki、Kaneko Mika K.、Kato Yukinari
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 40 Issue: 5 Pages: 219-226
  • DOI: 10.1089/mab.2021.0028
  • Peer Reviewed
• [Journal Article] Preclinical evaluation of podoplanin-targeted al-pha-radioimmunotherapy with the novel antibody NZ-16 for malignant mesothelioma (2021)
  • Author(s): Asano T, Suzuki H, Kaneko MK, Kato Y.
  • Journal Title: Cells Volume: 10 Issue: 10 Pages: 2503-2503
  • DOI: 10.3390/cells10102503
  • Peer Reviewed / Open Access
• [Journal Article] An Anti‐HER2 Monoclonal Antibody H2Mab‐41 Exerts Antitumor Activities in Mouse Xenograft Model using Dog HER2‐overexpressed Cells (2021)
  • Author(s): Tateyama N, Asano T, Ohishi T, Takei J, Hosono H, Nanamiya R, Tanaka T, Sano M, Saito M, Kawada M, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 184-190
  • DOI: 10.1089/mab.2021.0025
  • Peer Reviewed / Open Access
• [Journal Article] Defucosylated Anti-EGFR Monoclonal Antibody 134-mG2a-f Exerts Antitumor Activities in Mouse Xenograft Models of Dog EGFR-overexpressed Cells (2021)
  • Author(s): Tateyama N, Nanamiya R, Ohishi T, Takei J, Nakamura T, Yanaka M, Hosono H, Saito M, Asano T, Tanaka T, Sano M, Kawada M, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 177-183
  • DOI: 10.1089/mab.2021.0022
  • Peer Reviewed / Open Access
• [Journal Article] Development of a novel epitope mapping system: RIEDL insertion for epitope mapping method (2021)
  • Author(s): Asano T, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 162-167
  • DOI: 10.1089/mab.2021.0023
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of an Anti-Human EGFR Monoclonal Antibody (EMab-51) using the REMAP Method (2021)
  • Author(s): Nanamiya R, Sano M, Asano T, Yanaka M, Nakamura T, Saito M, Tanaka T, Hosono H, Tateyama N, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 149-155
  • DOI: 10.1089/mab.2021.0010
  • Peer Reviewed / Open Access
• [Journal Article] Development of a Novel Anti-HER2 Monoclonal Antibody H2Mab-181 for Gastric Cancer (2021)
  • Author(s): Takei J, Asano T, Tanaka T, Sano M, Hosono H, Nanamiya R, Tateyama N, Saito M, Suzuki H, Harada H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 168-176
  • DOI: 10.1089/mab.2021.0021
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of the Anti-CD44 Monoclonal Antibody (C44Mab-46) Using the REMAP Method (2021)
  • Author(s): Asano T, Kaneko MK, Takei J, Tateyama N, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 156-161
  • DOI: 10.1089/mab.2021.0012
  • Peer Reviewed
• [Journal Article] Epitope Mapping of an Anti-human EGFR Monoclonal Antibody (EMab-134) using the REMAP Method (2021)
  • Author(s): Sano M, Kaneko MK, Asano T, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 191-195
  • DOI: 10.1089/mab.2021.0014
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of the Anti-California Sea Lion Podoplanin Monoclonal Antibody PMab-269 using Alanine-scanning Mutagenesis and ELISA (2021)
  • Author(s): Tanaka T, Asano T, Sano M, Takei J, Hosono H, Nanamiya R, Tateyama N, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 4 Pages: 196-200
  • DOI: 10.1089/mab.2021.0017
  • Peer Reviewed
• [Journal Article] Anti-HER3 monoclonal antibody exerts antitumor activity in a mouse model of colorectal adenocarcinoma (2021)
  • Author(s): Asano T, Ohishi T, Takei J, Nakamura T, Nanamiya R, Hosono H, Tanaka T, Sano M, Harada H, Kawada M, Kaneko MK, Kato Y.
  • Journal Title: Oncol. Rep Volume: 46 Issue: 2 Pages: 173-173
  • DOI: 10.3892/or.2021.8124
  • Peer Reviewed / Open Access
• [Journal Article] Ferret Podoplanin is Detected by PMab-241 in Immunohistochemistry (2021)
  • Author(s): Nanamiya R, Takei J, Asano T, Sano M, Tanaka T, Hosono H, Harada H, Sakai Y, Mizuno T, Suzuki H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 3 Pages: 134-140
  • DOI: 10.1089/mab.2021.0016
  • Peer Reviewed
• [Journal Article] Development of Anti-Human CC Chemokine Receptor 9 Monoclonal Antibodies for Flow Cytometry (2021)
  • Author(s): Nanamiya R, Takei J, Asano T, Tanaka T, Sano M, Nakamura T, Yanaka M, Hosono H, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 3 Pages: 101-106
  • DOI: 10.1089/mab.2021.0007
  • Peer Reviewed
• [Journal Article] Development of an Anti-Elephant Podoplanin Monoclonal Antibody PMab-265 for Flow cytometry (2021)
  • Author(s): Hosono H, Asano T, Takei J, Sano M, Tanaka T, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 3 Pages: 141-145
  • DOI: 10.1089/mab.2021.0015
  • Peer Reviewed
• [Journal Article] Development of Monoclonal Antibody PMab-269 Against California Sea Lion Podoplanin (2021)
  • Author(s): Tanaka T, Asano T, Sano M, Takei J, Hosono H, Nanamiya R, Nakamura T, Yanaka M, Harada H, Fukui M, Suzuki H, Uchida K, Nakagawa T, Kato Y, Kaneko MK.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 3 Pages: 124-133
  • DOI: 10.1089/mab.2021.0011
  • Peer Reviewed
• [Journal Article] Development of Anti-Mouse CC Chemokine Receptor 3 Monoclonal Antibodies for Flow Cytometry (2021)
  • Author(s): Asano A, Nanamiya R, Takei J, Nakamura T, Yanaka M, Hosono H, Tanaka T, Sano M, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 3 Pages: 107-112
  • DOI: 10.1089/mab.2021.0009
  • Peer Reviewed
• [Journal Article] An anti-TROP2 monoclonal antibody TrMab-6 exerts antitumor activity in breast cancer mouse xenograft models (2021)
  • Author(s): Tanaka T, Ohishi T, Asano T, Takei J, Nanamiya R, Hosono H, Sano M, Harada H, Kawada M, Kaneko MK, Kato Y.
  • Journal Title: Oncol Rep Volume: 46 Issue: 1 Pages: 132-132
  • DOI: 10.3892/or.2021.8083
  • Peer Reviewed / Open Access
• [Journal Article] Development of Anti-Mouse CC chemokine receptor 8 Monoclonal Antibodies for Flow Cytometry (2021)
  • Author(s): Tanaka T, Nanamiya R, Takei J, Nakamura T, Yanaka M, Hosono H, Sano M, Asano T, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 2 Pages: 65-70
  • DOI: 10.1089/mab.2021.0005
• [Journal Article] Development of anti-human killer cell lectin-like receptor subfamily G member 1 monoclonal antibodies for flow cytometry (2021)
  • Author(s): Asano T, Nanamiya R, Tanaka T, Kaneko MK, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 2 Pages: 76-80
  • DOI: 10.1089/mab.2021.0008
  • Peer Reviewed
• [Journal Article] Development of Anti-Human T-Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) Monoclonal Antibodies for Flow Cytometry (2021)
  • Author(s): Takei J, Asano T, Nanamiya R, Nakamura T, Yanaka M, Hosono H, Tanaka T, Sano M, Kaneko MK, Harada H, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 40 Issue: 2 Pages: 71-75
  • DOI: 10.1089/mab.2021.0006
  • Peer Reviewed
• [Journal Article] Establishment of a novel anti-TROP2 monoclonal antibody TrMab-29 for immunohistochemical analysis (2021)
  • Author(s): Sayama Y, Kaneko MK, Takei J, Hosono H, Sano M, Asano T, Kato Y.
  • Journal Title: Biochem Biophys Rep Volume: 25 Pages: 100902-100902
  • DOI: 10.1016/j.bbrep.2020.100902
  • Peer Reviewed / Open Access
• [Journal Article] Development and characterization of TrMab-6, a novel anti-TROP2 monoclonal antibody for antigen detection in breast cancer (2021)
  • Author(s): Sayama Y, Kaneko MK, Kato Y.
  • Journal Title: Mol Med Rep Volume: 23 Issue: 2 Pages: 92-92
  • DOI: 10.3892/mmr.2020.11731
  • Peer Reviewed / Open Access
• [Journal Article] The Anti-EpCAM monoclonal antibody (EpMab-16) exerts anti-tumor activity against oral squamous cell carcinomas (2020)
  • Author(s): Kaneko MK, Ohishi T, Takei J, Sano M, Nakamura T, Hosono H, Yanaka M, Asano T, Sayama Y, Harada H, Kawada M, Kato Y
  • Journal Title: Oncol Rep Volume: 44 Issue: 6 Pages: 2517-2526
  • DOI: 10.3892/or.2020.7808
  • Peer Reviewed / Open Access
• [Journal Article] The anti-EpCAM monoclonal antibody EpMab-16 exerts antitumor activity in a mouse model of colorectal adenocarcinoma (2020)
  • Author(s): Hosono H, Ohishi T, Takei J, Asano T, Sayama Y, Kawada M, Kaneko MK, Kato Y
  • Journal Title: Oncol Lett Volume: 20 Issue: 6 Pages: 383-383
  • DOI: 10.3892/ol.2020.12246
  • Peer Reviewed / Open Access
• [Journal Article] Development of Core-fucose Deficient Humanized and Chimeric Anti-Human Podoplanin Antibodies (2020)
  • Author(s): Kaneko MK, Ohishi T, Nakamura T, Inoue H, Takei J, Sano M, Asano T, Sayama Y, Hosono H, Suzuki H, Kawada M, Kato Y.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother Volume: 39 Issue: 5 Pages: 167-174
  • DOI: 10.1089/mab.2020.0019
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide (2020)
  • Author(s): Ogasawara S, Suzuki K, Naruchi K, Nakamura S, Shimabukuro J, Tsukahara N, Kaneko MK, Kato Y, Murata T
  • Journal Title: Biochem Biophys Res Commun. Volume: 533 Issue: 1 Pages: 57-63
  • DOI: 10.1016/j.bbrc.2020.08.103
  • Peer Reviewed / Open Access
• [Journal Article] A cancer-specific anti-podocalyxin monoclonal antibody (60-mG2a-f) exerts antitumor effects in mouse xenograft models of pancreatic carcinoma (2020)
  • Author(s): Kaneko MK, Ohishi T, Kawada M, Kato Y
  • Journal Title: Biochem Biophys Rep Volume: 24 Pages: 100826-100826
  • DOI: 10.1016/j.bbrep.2020.100826
  • Peer Reviewed / Open Access
• [Journal Article] A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma (2020)
  • Author(s): Takei J, Kaneko MK, Ohishi T, Hosono H, Nakamura T, Yanaka M, Sano M, Asano T, Sayama Y, Kawada M, Harada H, Kato Y
  • Journal Title: Oncol Rep. Volume: 44 Pages: 1949-1960
  • DOI: 10.3892/or.2020.7735
  • Peer Reviewed / Open Access
• [Journal Article] Epitope mapping of an anti-diacylglycerol kinase delta monoclonal antibody DdMab-1 (2020)
  • Author(s): Sano M, Asano T, Kaneko MK, Kato Y
  • Journal Title: Biochem Biophys Rep Volume: 24 Pages: 100808-100808
  • DOI: 10.1016/j.bbrep.2020.100808
  • Peer Reviewed / Open Access
• [Journal Article] A defucosylated anti-PD-L1 monoclonal antibody 13-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma (2020)
  • Author(s): Takei J, Ohishi T, Kaneko MK, Harada H, Kawada M, Kato Y
  • Journal Title: Biochem Biophys Rep Volume: 24 Pages: 100801-100801
  • DOI: 10.1016/j.bbrep.2020.100801
  • Peer Reviewed / Open Access
• [Journal Article] The anti-EGFR monoclonal antibody 134-mG2a exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma (2020)
  • Author(s): Hosono H, Takei J, Ohishi T, Sano M, Asano T, Sayama Y, Nakamura T, Yanaka M, Kawada M, Harada H, Kaneko MK, Kato Y
  • Journal Title: Int J Mol Med Volume: 46 Pages: 1443-1452
  • DOI: 10.3892/ijmm.2020.4700
  • Peer Reviewed / Open Access
• [Journal Article] RIEDL tag: A novel pentapeptide tagging system for transmembrane protein purification (2020)
  • Author(s): Asano T, Kaneko MK, Kato Y
  • Journal Title: Biochem Biophys Rep Volume: 23 Pages: 100780-100780
  • DOI: 10.1016/j.bbrep.2020.100780
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of C20Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells (2020)
  • Author(s): Furusawa Y, Kaneko MK, Kato Y
  • Journal Title: Oncol Lett Volume: 20 Issue: 2 Pages: 1961-1967
  • DOI: 10.3892/ol.2020.11753
  • Peer Reviewed / Open Access
• [Journal Article] H2Mab-19, an anti-human epidermal growth factor receptor 2 monoclonal antibody exerts antitumor activity in mouse oral cancer xenografts (2020)
  • Author(s): Takei J, Kaneko MK, Ohishi T, Kawada M, Harada H, Kato Y
  • Journal Title: Exp Ther Med Volume: 20 Issue: 2 Pages: 846-853
  • DOI: 10.3892/etm.2020.8765
  • Peer Reviewed / Open Access
• [Journal Article] A novel anti-EGFR monoclonal antibody (EMab-17) exerts antitumor activity against oral squamous cell carcinomas via antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity (2020)
  • Author(s): Takei J, Kaneko MK, Ohishi T, Kawada M, Harada H, Kato Y
  • Journal Title: Oncol Lett Volume: 19 Pages: 2809-2816
  • DOI: 10.3892/ol.2020.11384
  • Peer Reviewed / Open Access
• [Journal Article] Epitope Mapping of PMab-225 an Anti-Alpaca Podoplanin Monoclonal Antibody using Flow Cytometry (2019)
  • Author(s): Sayama Y, Sano M, Furusawa Y, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 6 Pages: 255-260
  • DOI: 10.1089/mab.2019.0033
  • Peer Reviewed
• [Journal Article] PMab-241 Specifically Detects Bear Podoplanin of Lymphatic endothelial cells in the Lung of Brown Bear (2019)
  • Author(s): Takei J, Yamada S, Konnai S, Ishinazaka T, Shimozuru M, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 6 Pages: 282-284
  • DOI: 10.1089/mab.2019.0038
  • Peer Reviewed
• [Journal Article] The Epitope of PMab-210 is Located in Platelet Aggregation-Stimulating Domain-3 of Pig Podoplanin (2019)
  • Author(s): Kaneko MK, Sayama Y, Sano M, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 6 Pages: 271-276
  • DOI: 10.1089/mab.2019.0037
  • Peer Reviewed
• [Journal Article] Epitope Analysis of an Anti-Horse Podoplanin Monoclonal Antibody PMab-219 (2019)
  • Author(s): Kato Y, Sayama Y, Sano M, Kaneko MK
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 6 Pages: 266-270
  • DOI: 10.1089/mab.2019.0034
  • Peer Reviewed
• [Journal Article] Epitope Mapping of Monoclonal Antibody PMab-233 Against Tasmanian Devil Podoplanin (2019)
  • Author(s): Sano M, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 6 Pages: 261-265
  • DOI: 10.1089/mab.2019.0032
  • Peer Reviewed
• [Journal Article] Epitope Mapping of Anti-Bear Podoplanin Monoclonal Antibody PMab-247 (2019)
  • Author(s): Kato Yukinari、Takei Junko、Furusawa Yoshikazu、Sayama Yusuke、Sano Masato、Konnai Satoru、Kobayashi Atsushi、Harada Hiroyuki、Takahashi Maki、Suzuki Hiroyoshi、Yamada Shinji、Kaneko Mika K.
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 38 Issue: 5 Pages: 230-233
  • DOI: 10.1089/mab.2019.0025
  • Peer Reviewed
• [Journal Article] Epitope mapping of anti-pig podoplanin monoclonal antibody PMab-213 (2019)
  • Author(s): Yamada S, Itai S, Furusawa Y, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 5 Pages: 224-229
  • DOI: 10.1089/mab.2019.0023
  • Peer Reviewed
• [Journal Article] Characterization of anti-goat podoplanin monoclonal antibody PMab-235 using immunohistochemistry against goat tissues (2019)
  • Author(s): Takei J, Itai S, Harada H, Furusawa Y, Miwa T, Fukui M, Nakamura T, Sano M, Sayama Y, Yanaka M, Handa S, Hisamatsu K, Nakamura Y, Yamada S, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 5 Pages: 213-219
  • DOI: 10.1089/mab.2019.0022
  • Peer Reviewed
• [Journal Article] PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis (2019)
  • Author(s): Takei Junko、Furusawa Yoshikazu、Yamada Shinji、Nakamura Takuro、Sayama Yusuke、Sano Masato、Konnai Satoru、Kobayashi Atsushi、Harada Hiroyuki、Kaneko Mika K.、Kato Yukinari
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 38 Issue: 4 Pages: 171-174
  • DOI: 10.1089/mab.2019.0019
  • Peer Reviewed
• [Journal Article] Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer (2019)
  • Author(s): Kato Yukinari、Ohishi Tomokazu、Yamada Shinji、Itai Shunsuke、Takei Junko、Sano Masato、Nakamura Takuro、Harada Hiroyuki、Kawada Manabu、Kaneko Mika K.
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 38 Issue: 4 Pages: 157-161
  • DOI: 10.1089/mab.2019.0017
  • Peer Reviewed
• [Journal Article] Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis (2019)
  • Author(s): Furusawa Y, Takei J, Sayama Y, Yamada S, Kaneko MK, Kato Y
  • Journal Title: Biochem Biophys Rep. Volume: 18 Pages: 100644-100644
  • DOI: 10.1016/j.bbrep.2019.100644
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a monoclonal antibody PMab-225 against alpaca podoplanin for immunohistochemical analyses (2019)
  • Author(s): Kato Y, Furusawa Y, Yamada S, Itai S, Takei J, Sano M, Kaneko MK
  • Journal Title: Biochem Biophys Rep. Volume: 18 Pages: 100633-100633
  • DOI: 10.1016/j.bbrep.2019.100633
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin (2019)
  • Author(s): Furusawa Y, Yamada S, Itai S, Nakamura T, Takei J, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y
  • Journal Title: Biochem Biophys Rep. Volume: 18 Pages: 100631-100631
  • DOI: 10.1016/j.bbrep.2019.100631
  • Peer Reviewed / Open Access
• [Journal Article] PMab-235: a monoclonal antibody for immunohistochemical analysis against goat podoplanin (2019)
  • Author(s): Furusawa Y, Yamada S, Nakamura T, Sano M, Sayama Y, Itai S, Takei J, Harada H, Fukui M, Kaneko MK, Kato Y
  • Journal Title: Heliyon Volume: 5 Issue: 7 Pages: e02063-e02063
  • DOI: 10.1016/j.heliyon.2019.e02063
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis (2019)
  • Author(s): Kato Y, Furusawa Y, Itai S, Takei J, Nakamura T, Sano M, Harada H, Yamada S, Kaneko MK.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 3 Pages: 108-113
  • DOI: 10.1089/mab.2019.0013
  • Peer Reviewed
• [Journal Article] Epitope mapping of anti-tiger podoplanin monoclonal antibody PMab-231 (2019)
  • Author(s): Takei J, Itai S, Furusawa Y, Yamada S, Nakamura T, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 3 Pages: 129-132
  • DOI: 10.1089/mab.2019.0012
  • Peer Reviewed
• [Journal Article] Anti-CD133 Monoclonal Antibody CMab-43 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer (2019)
  • Author(s): Kato Yukinari、Ohishi Tomokazu、Yamada Shinji、Itai Shunsuke、Furusawa Yoshikazu、Sano Masato、Nakamura Takuro、Kawada Manabu、Kaneko Mika K.
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 38 Issue: 2 Pages: 75-78
  • DOI: 10.1089/mab.2019.0002
  • Peer Reviewed
• [Journal Article] Establishment of a monoclonal antibody PMab-231 for tiger podoplanin (2019)
  • Author(s): Furusawa Y, Kaneko MK, Nakamura T, Itai S, Fukui M, Harada H, Yamada S, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 2 Pages: 89-95
  • DOI: 10.1089/mab.2019.0003
  • Peer Reviewed
• [Journal Article] Epitope mapping of the anti-horse podoplanin monoclonal antibody PMab-202 (2019)
  • Author(s): Kaneko MK, Furusawa Y, Sano M, Itai S, Takei J, Harada H, Fukui M, Yamada S, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 2 Pages: 79-84
  • DOI: 10.1089/mab.2019.0001
  • Peer Reviewed
• [Journal Article] Anti-bovine podoplanin monoclonal antibody PMab-44 detects goat podoplanin in immunohistochemistry (2019)
  • Author(s): Yamada S, Kaneko MK, Furusawa Y, Itai S, Sano M, Nakamura T, Yanaka M, Handa S, Hisamatsu K, Nakamura Y, Koyanagi M, Fukui M, Harada H, Kato Y
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 2 Pages: 96-99
  • DOI: 10.1089/mab.2018.0031
  • Peer Reviewed
• [Journal Article] PMab-210: A Monoclonal Antibody Against Pig Podoplanin (2019)
  • Author(s): Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Fukui M, Harada H, Mizuno T, Sakai Y, Takasu M, Kaneko MK, Kato Y*.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 1 Pages: 30-36
  • DOI: 10.1089/mab.2018.0038
  • Peer Reviewed
• [Journal Article] PMab-213: a monoclonal antibody for immunohistochemical analysis against pig podoplanin (2019)
  • Author(s): Kato Y, Yamada S, Furusawa Y, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 38 Issue: 1 Pages: 18-24
  • DOI: 10.1089/mab.2018.0048
  • Peer Reviewed
• [Journal Article] PMab-219: a monoclonal antibody for the immunohistochemical analysis of horse podoplanin (2019)
  • Author(s): Furusawa Y, Yamada S, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y
  • Journal Title: Biochem Biophys Rep. Volume: 18 Pages: 100616-100616
  • DOI: 10.1016/j.bbrep.2019.01.009
  • Peer Reviewed / Open Access
• [Journal Article] The mouse-canine chimeric anti-dog podoplanin antibody P38B exerts antitumor activity in mouse xenograft models (2019)
  • Author(s): Kato Y, Ohishi T, Kawada M, Maekawa N, Konnai S, Itai S, Yamada S, Kaneko MK.
  • Journal Title: Biochem Biophys Rep. Volume: 17 Pages: 23-26
  • DOI: 10.1016/j.bbrep.2018.11.005
  • Peer Reviewed / Open Access
• [Presentation] ペプチドタグシステムを用いた新規抗体エピトープ解析法の開発 (2021)
  • Author(s): 加藤幸成、淺野禎三、金子美華
  • Organizer: 第21回 日本蛋白質科学会年会
  • Invited
• [Presentation] ヒト抗体医薬の現状と未来 (2021)
  • Author(s): 加藤幸成
  • Organizer: 第12回動物用ワクチン・バイオ医薬研究会シンポジウム『動物用バイオ医薬品の最先端：動物用抗体医薬の現状と未来』
  • Invited
• [Presentation] 産学連携を基軸としたがん特異的抗体の開発 (2020)
  • Author(s): 加藤幸成
  • Organizer: 第24回日本がん分子標的治療学会学術集会
  • Invited
• [Presentation] がん幹細胞マーカーCD133を標的とした抗体創薬 (2020)
  • Author(s): 加藤 幸成, 金子 美華, 大石 智一, 川田 学
  • Organizer: 第93回日本薬理学会年会
• [Presentation] A novel monoclonal antibody for a cancer stem cell marker CD133 exerts anti-tumor and anti-metastasis activities for colon cancers (2019)
  • Author(s): Yukinari Kato, Tomokazu Ohishi, Manabu Kawada, Mika K. Kaneko
  • Organizer: AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics 2019
  • Int'l Joint Research
• [Presentation] がん特異的糖ペプチドを認識する新規ラット抗ポドプラニン抗体の樹立 (2019)
  • Author(s): 加藤幸成, 金子美華, 山田慎二
  • Organizer: 第７８回日本癌学会学術総会
• [Presentation] がん幹細胞マーカーCD133を標的とした抗体創薬 (2019)
  • Author(s): 加藤幸成、山田慎二、大石智一、川田 学、金子美華
  • Organizer: 第28回日本がん転移学会
• [Presentation] LpMab-23: an anti-podoplanin cancer-specific antibody for a prognostic marker of oral cancer (2019)
  • Author(s): Takashi Miwa, Shinji Yamada, Mika K. Kaneko, Yoshikazu Furusawa, Masato Fukui, Yukinari Kato
  • Organizer: Annual meeting of AACR 2019
  • Int'l Joint Research
• [Presentation] 抗体関連高度開発支援と糖鎖細胞工学による高度化 (2019)
  • Author(s): 加藤幸成
  • Organizer: 第42回日本分子生物学会年会
  • Invited
• [Presentation] がん特異的糖鎖修飾による構造認識抗体の開発とその臨床応用 (2019)
  • Author(s): 加藤幸成
  • Organizer: 第410回CBI学会講演会
  • Invited
• [Presentation] 生化学研究のための高機能抗体の開発 (2019)
  • Author(s): 第92回日本日本生化学会大会
  • Organizer: 加藤幸成, 金子美華, 山田慎二
  • Invited
• [Remarks] 東北大学大学院医学系研究科分子薬理学分野・業績
  • URL: http://www.med-tohoku-antibody.com/reference/01.htm
• [Remarks] 抗体創薬研究分野
  • URL: http://www.med-tohoku-antibody.com/index.htm
• [Remarks] 東北大学大学院医学系研究科抗体創薬研究分野
  • URL: http://www.med-tohoku-antibody.com/index.htm