| Project/Area Number |
19K07843
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 51030:Pathophysiologic neuroscience-related
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
WALKER DOUGLAS 滋賀医科大学, 神経難病研究センター, 特任教授 (10813694)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Project Status |
Discontinued (Fiscal Year 2020)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | TFEB / alpha synuclein / Lewy body / autophagy / lysozome / tau / Parkinson's disease / Alzheimer's disease / polo-like kinase / transcription factor / phosphorylation |
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| Outline of Research at the Start |
The aim of this project is to demonstrate that enhancement of autophagy/lysosomal function through activation of Transcription Factor EB (TFEB) will increase the degradation of forms of alpha synuclein , particularly phosphorylated alpha synuclein, before it becomes deposited into Lewy bodies.
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| Outline of Annual Research Achievements |
The aim of this project is to demonstrate that enhancement of autophagy/lysosomal
function through activation of Transcription Factor EB (TFEB) will increase the degradation of intracellular forms of disease-related proteins such as phosphorylated alpha synuclein (p-syn) and tau (p-tau). We examined the expression of progranulin (PGRN) and prosaposin (PSAP), regulators of lysosomal function, in the brain of Alzheiemer's disease (AD), and found the association of PGRN and PSAP with amyloid plaques in non-demented aged control and AD brains. Next, we investigated the possible involvement of PGRN and PSAP in tangle formation using human brain tissue sections of non-demented aged control subjects and AD cases, and compared with cases of fronto-temporal dementia (FTD) with granulin (GRN) mutations. The study revealed that decreased amounts of PGRN and PSAP proteins were detected in immature neurofibrillary tangles, while colocalization was still evident in adjacent neurons in all cases. Results suggest that neuronal loss of PGRN preceded loss of PSAP as tangles matured.The granulin mutation cases exhibited absence of PGRN in most neurons, while PSAP signal was preserved. We conclude that reduced levels of PGRN and PSAP and their interaction in neurons might predispose to accumulation of p-tau protein due to deficits in lysosomal activity.
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