Epigenomic analysis in refractory AML

• Project/Area Number: 19K08350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52050:Embryonic medicine and pediatrics-related
• Research Institution: Yokohama City University
• Principal Investigator: SHIBA Norio 横浜市立大学, 附属病院, 講師 (50600615)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 急性骨髄性白血病 / DNAメチル化 / ATAC-seq / micro RNA / Chipシーケンス / microRNAシーケンス / 遺伝子解析 / ATACシーケンス

Outline of Research at the Start

小児急性骨髄性白血病(AML)は小児がんの中でも予後不良な疾患の1つである。これまでの網羅的な遺伝子解析の結果、予後不良な遺伝子再構成であるNUP98-NSD1症例においてPRDM16 (MEL1) 遺伝子が高発現していることを同定し、PRDM16高発現症例ではPRDM16遺伝子全長に渡ってポリコーム遺伝子群との結合部位が高度にメチル化されており、interactionが阻害されている可能性を見出した。ポリコーム遺伝子群はHOX遺伝子群に作用し、幹細胞制御にかかわる重要な遺伝子群であり、これらの関係を明らかにし、創薬につながる分子標的の同定を試みたいと考えている。

Outline of Final Research Achievements

CHIP-seq was performed using a sample with acute myeloid leukemia harboring NUP98-NSD1 with high expression of PRDM16. As a result, some novel candidate genes were identified, in addition to known cancer-related genes such as SKI and RUNX1. ATAC-seq found that the promoter region of PRDM16 is open in the AML samples showing PRDM16 high expression. Intriguingly, same region showed low DNA methylation pattern, although gene body of PRDM16 gene itself was highly methylated resulting in inhibiting binding with Polycomb complex. These alteration contributes to the refractory AML. Furthermore, microRNA sequences were performed on 48 pediatric AMLs with carrying various genetic backgrounds. They classified them into four clusters based on expression patterns, some of which correlated with existing genetic abnormalities.

Academic Significance and Societal Importance of the Research Achievements

難治AML症例より同定された遺伝子異常の機能的な意義を解明することで、AMLの発症原因を解明し、創薬開発に繋げていくことで難治AML患者を救命することが可能となる。層別化については、これまでの解析にて概ね完了している。しかしながら、まだ約10％の患者においては、driverとなる遺伝子異常を見いだすことが出来ていない（これらの症例の多くは複雑な染色体の構造異常を認め、予後不良である）。よって、より高精度の治療層別化フローチャートを作成し、すべての小児AML症例の再発リスク、予後予測に基づいた適切な治療強度を選択できるようにするため、これらの10％の症例の発病原因を探索することに注力する。

Research Products

• [Journal Article] Genome-wide DNA Methylation Analysis in Pediatric Acute Myeloid Leukemia. (2022)
  • Author(s): Yamato G, Kawai T, Shiba N, Ikeda J, Hara Y, Ohki K, Tsujimoto SI, Kaburagi T, Yoshida K, Shiraishi Y, Miyano S, Kiyokawa N, Tomizawa D, Shimada A, Sotomatsu M, Arakawa H, Adachi S, Taga T, Horibe K, Ogawa S, Hata K, Hayashi Y.
  • Journal Title: Blood advances Volume: in press Issue: 11 Pages: 3207-3219
  • DOI: 10.1182/bloodadvances.2021005381
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Droplet digital polymerase chain reaction assay for the detection of the minor clone of KIT D816V in paediatric acute myeloid leukaemia especially showing RUNX1-RUNX1T1 transcripts. (2021)
  • Author(s): Sasaki K, Tsujimoto S, Miyake M, Uchiyama Y, Ikeda J, Yoshitomi M, Shimosato Y, Tokumasu M, Matsuo H, Yoshida K, Ohki K, Kaburagi T, Yamato G, Hara Y, Takeuchi M, Kinoshita A, Tomizawa D, Taga T, Adachi S, Tawa A, Horibe K, Hayashi Y, Matsumoto N, Ito S, Shiba N.
  • Journal Title: British Journal of Haematology Volume: 194(2) Issue: 2 Pages: 414-422
  • DOI: 10.1111/bjh.17569
  • Peer Reviewed
• [Journal Article] Clinical significance of RAS pathway alterations in pediatric acute myeloid leukemia. (2021)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Yoshida K, Hara Y, Tabuchi K, Shiraishi Y,Ohki K, Sotomatsu M, Arakawa H, Matsuo H, Shimada A, Taki T, Kiyokawa N, Tomizawa D, Horibe K, Miyano S, Taga T, Adachi S, Ogawa S, Hayashi Y.
  • Journal Title: Haematologica Volume: － Issue: 3 Pages: 583-592
  • DOI: 10.3324/haematol.2020.269431
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Predisposition to prolonged neutropenia after chemotherapy for paediatric acute myeloid leukaemia is associated with better prognosis in the Japanese Paediatric Leukaemia/Lymphoma Study Group AML‐05 study. (2020)
  • Author(s): Aoki T, Takahashi H, Tanaka S, Shiba N, Hasegawa D, Iwamoto S, Terui K, Moritake H, Nakayama H, Shimada A, Koh K, Goto H, Kosaka Y, Saito AM, Horibe K, Kinoshita A, Tawa A, Taga T, Adachi S, Tomizawa D.
  • Journal Title: British Journal of Haematology Volume: 193 Issue: 1 Pages: 176-180
  • DOI: 10.1111/bjh.16656
  • Peer Reviewed
• [Journal Article] The outcomes of relapsed acute myeloid leukemia in children: Results from the Japanese Pediatric Leukemia/Lymphoma Study Group AML‐05R study. (2020)
  • Author(s): Moritake H, Tanaka S, Miyamura T, Nakayama H, Shiba N, Shimada A, Terui K, Yuza Y, Koh K, Goto H, Kakuda H, Saito A, Hasegawa D, Iwamoto S, Taga T, Adachi S, Tomizawa D.
  • Journal Title: Pediatric Blood & Cancer Volume: 68 Issue: 1
  • DOI: 10.1002/pbc.28736
  • Peer Reviewed
• [Journal Article] Patients aged less than 3 years with acute myeloid leukaemia characterize a molecularly and clinically distinct subgroup. (2020)
  • Author(s): Hara Y, Shiba N, Yamato G, Ohki K, Tabuchi K, Sotomatsu M, Tomizawa D, Kinoshita A, Arakawa H, Saito AM, Kiyokawa N, Tawa A, Horibe K, Taga T, Adachi S, Taki T, Hayashi Y
  • Journal Title: Br J Haematol Volume: 188 Issue: 4 Pages: 528-539
  • DOI: 10.1111/bjh.16203
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A 2‐year‐old patient with a diffuse intrinsic pontine glioma and radiation‐induced moyamoya syndrome. (2020)
  • Author(s): Iizuka A, Shiba N, Shimosato Y, Yoshitomi M, Nakamura T, Miyatake S, Takano Y, Sasaki K, Takeuchi M, Nurata H, Yamamoto N, Ito S.
  • Journal Title: Pediatric Blood & Cancer Volume: 67 Issue: 10
  • DOI: 10.1002/pbc.28618
  • Peer Reviewed
• [Journal Article] A Hyperactive RelA/p65-Hexokinase 2 Signaling Axis Drives Primary Central Nervous System Lymphoma (2020)
  • Author(s): Tateishi Kensuke、Miyake Yohei、Kawazu Masahito、Sasaki Nobuyoshi、Nakamura Taishi、Sasame Jo、Yoshii Yukie、Ueno Toshihide、Miyake Akio、Matsushita Yuko、Shiba Norio、Udaka Naoko、Ohki Kentaro、Fink Alexandria L.、Tummala Shilpa S.、Natsumeda Manabu、Miller Julie J.、Fujii Yukihiko、et al
  • Journal Title: Cancer Research Volume: 80 Issue: 23 Pages: 5330-5343
  • DOI: 10.1158/0008-5472.can-20-2425
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Allogeneic Bone Marrow Transplantation versus Peripheral Blood Stem Cell Transplantation for Hematologic Malignancies in Children: A Systematic Review and Meta-Analysis. (2020)
  • Author(s): Shimosato Y, Tanoshima R, Tsujimoto SI, Takeuchi M, Shiba N, Kobayashi T, Ito S.
  • Journal Title: Biology of Blood and Marrow Transplantation Volume: 26 Issue: 1 Pages: 88-93
  • DOI: 10.1016/j.bbmt.2019.07.025
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome analysis offers a comprehensive illustration of the genetic background of pediatric acute myeloid leukemia (2019)
  • Author(s): Shiba N, Yoshida K, Hara Y, Yamato G, Shiraishi Y, Matsuo H, Okuno Y, Chiba K, Tanaka H, Kaburagi T, Takeuchi M, Ohki K, Sanada M, Okubo J, Tomizawa D, Taki T, Shimada A, Sotomatsu M, Horibe K, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y
  • Journal Title: Blood Adv Volume: 3 Issue: 20 Pages: 3157-3169
  • DOI: 10.1182/bloodadvances.2019000404
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Negative CD19 expression is associated with inferior relapse‐free survival in children with RUNX1‐RUNX1T1 ?positive acute myeloid leukaemia: results from the Japanese Paediatric Leukaemia/Lymphoma Study Group AML‐05 study (2019)
  • Author(s): Sakamoto Kenichi、Shiba Norio、Deguchi Takao、Kiyokawa Nobutaka、Hashii Yoshiko、Moriya‐Saito Akiko、Tomizawa Daisuke、Taga Takashi、Adachi Soichi、Horibe Keizo、Imamura Toshihiko
  • Journal Title: British Journal of Haematology Volume: 187 Issue: 3 Pages: 372-376
  • DOI: 10.1111/bjh.16080
  • Peer Reviewed
• [Journal Article] Whole transcriptome sequencing reveals a KMT2A‐USP2 fusion in infant acute myeloid leukemia (2019)
  • Author(s): Ikeda Junji、Shiba Norio、Tsujimoto Shin‐ichi、Yoshida Masanori、Nakabayashi Kazuhiko、Ogata‐Kawata Hiroko、Okamura Kohji、Takeuchi Masanobu、Osumi Tomoo、Tomizawa Daisuke、Hata Kenichiro、Kiyokawa Nobutaka、Ito Shuichi、Kato Motohiro
  • Journal Title: Genes, Chromosomes and Cancer Volume: in press Issue: 9 Pages: 1-1
  • DOI: 10.1002/gcc.22751
  • Peer Reviewed / Open Access
• [Journal Article] Clinical and biological features of paediatric acute myeloid leukaemia (AML) with primary induction failure in the Japanese Paediatric Leukaemia/Lymphoma Study Group AML-05 study. (2019)
  • Author(s): Miyamura T, Moritake H, Nakayama H, Tanaka S, Tomizawa D, Shiba N, Saito AM, Tawa A, Shimada A, Iwamoto S, Hayashi Y, Koike T, Horibe K, Manabe A, Mizutani S, Taga T, Adachi S.
  • Journal Title: Br J Haematol. Volume: 19 Issue: 2 Pages: 284-288
  • DOI: 10.1111/bjh.15799
  • Peer Reviewed
• [Journal Article] Comparison of conditioning regimens for autologous stem cell transplantation in children with acute myeloid leukemia: A nationwide retrospective study in Japan (2018)
  • Author(s): Sakaguchi Hirotoshi、Muramatsu Hideki、Hasegawa Daiichiro、Kudo Kazuko、Ishida Hiroyuki、Yoshida Nao、Koh Katsuyoshi、Noguchi Maiko、Shiba Norio、Tokimasa Sadao、Fukuda Takhiro、Goto Hiroaki、Miyamura Takako、Nakazawa Yozo、Hashii Yoshiko、Inoue Masami、Atsuta Yoshiko、et al
  • Journal Title: Pediatric Blood & Cancer Volume: 66 Issue: 1
  • DOI: 10.1002/pbc.27459
  • Peer Reviewed / Open Access
• [Journal Article] Allogeneic hematopoietic stem cell transplantation for children and adolescents with high-risk cytogenetic AML: distinctly poor outcomes of FUS-ERG-positive cases (2018)
  • Author(s): Tomizawa Daisuke、Yoshida Masanori、Kondo Tadakazu、Miyamura Takako、Taga Takashi、Adachi Souichi、Koh Katsuyoshi、Noguchi Maiko、Kakuda Harumi、Watanabe Kenichiro、Cho Yuko、Fukuda Takahiro、Kato Motohiro、Shiba Norio、Goto Hiroaki、Okada Keiko、Inoue Masami、Hashii Yoshiko、Atsuta Yoshiko、Ishida Hiroyuki
  • Journal Title: Bone Marrow Transplantation Volume: 54 Issue: 3 Pages: 393-401
  • DOI: 10.1038/s41409-018-0273-7
  • Peer Reviewed
• [Presentation] Clonal Evolution Pattern and Prognostic Significance of Clonal Architecture in KMT2A-Rearranged Acute Myeloid Leukemia. (2021)
  • Author(s): Matsuo H, Yoshida K, Nannya Y, Ito Y, Saito S, Koga Y, Moritake H, Terui K, Kawaguchi K, Okamoto Y, Nakayama H, Kanno M, Hino M, Akane Y, Inoue A, Shimada A, Goto H, Ueno H, Takita J, Yamato G, Shiba N, Hayashi Y, Shiraishi Y, Miyano S, Kiyokawa N, Tomizawa D, Taga T, Tawa A, Ogawa S and Adachi S
  • Organizer: The 63rd American Society of Hematology Annual Meeting and Exposition(virtual)
  • Int'l Joint Research
• [Presentation] Cytokine Analysis in 154 Patients with Transient Abnormal Myelopoiesis: Jccg JPLSG TAM-10 Clinical Study. (2021)
  • Author(s): Yamato G, MD, Park MJ, Shimada A, Shiba N, Yamada Y, Terui K, Ito E, MD, Muramatsu H, Watanabe K, and Hayashi Y.
  • Organizer: The 63rd American Society of Hematology Annual Meeting and Exposition(virtual)
  • Int'l Joint Research
• [Presentation] A pediatric case of acute lymphoblastic leukemia with DVT and PYE（T細胞性急性リンパ性白血病治療中に深部静脈血栓症・肺動脈血栓塞栓症を合併した1例）. (2020)
  • Author(s): 飯塚淳広，下里侑子，吉富誠弘，佐々木康二，竹内正宣，柴 徳生，伊藤秀一
  • Organizer: 第123回日本小児科学会学術総会, 神戸
• [Presentation] 内部制度管理における試験管法目合わせの取り組み． (2020)
  • Author(s): 岩上 薫，瀧澤かすみ，黒沢英里，小峰弘美，原田佐保，山崎悦子，柴 徳生，上條亜紀
  • Organizer: 第68回日本輸血・細胞治療学会学術総会，札幌
• [Presentation] 当院における新生児貧血5例に対する診断および治療戦略． (2020)
  • Author(s): 柴 徳生，瀧澤かすみ，黒沢英里，小峰弘美，岩上 薫，原田佐保，飯塚淳広，吉富誠弘，佐々木康二，剱持孝博、魚住 梓，竹内正宣，伊藤秀一
  • Organizer: 第68回日本輸血・細胞治療学会学術総会，札幌
• [Presentation] Thiotepaによる皮膚障害の小児例． (2020)
  • Author(s): 竹内正宣，林 弘明，吉富誠弘，飯塚敦広，辻本信一，田野島玲大，柴 徳生，渡邊友也，伊藤秀一
  • Organizer: 第47回日本小児臨床薬理学会，前橋（Web）
• [Presentation] 服薬不遵守は国境を超えた問題である - 日加双方での小児がん患者の内服抗腫瘍薬服薬不遵守の経験を通して - (2020)
  • Author(s): 田野島玲大，竹内正宣，吉富誠弘，Bruce C Carleton，佐々木康二，柴 徳生，伊藤秀一
  • Organizer: 第47回日本小児臨床薬理学会学術集会，前橋（Web）
• [Presentation] Outcome of relapsed pediatric acute leukemia in Japan: The results of 111 cases from the JPLSG AML-05R study（本邦における再発小児急性骨髄性白血病の予後：JPLSG AML-05R登録111例の解析）. (2020)
  • Author(s): Moritake H, Tanaka S, Miyamura T, Nakayama H, Shiba N, Hasegawa D, Iwamoto S, Taga T, Adachi S, Tomizawa D
  • Organizer: 第62回日本小児血液・がん学会学術集会，福島（ Web）
• [Presentation] High gene expression of DOCK1 shows poor prognosis in children with acute myeloid leukemia（小児急性骨髄性白血病においてDOCK1遺伝子高発現は予後不良因子となり得る）． (2020)
  • Author(s): Yoshitomi M, Ikeda J, Tsujimoto S, Ito S, Hasegawa D, Iwamoto S, Hayashi Y, Adachi S, Tomizawa D, Shiba N
  • Organizer: 第62回日本小児血液・がん学会学術集会，福島（ Web）
• [Presentation] The genetic landscape of pediatric acute promyelocytic leukemia - the JCCG study, JPLSG AML-P05 -（小児急性骨髄性白血病に対するカスタムパネルを用いた網羅的遺伝子解析 - the JCCG study, JPLSG AML-P05 - (2020)
  • Author(s): Yamato G, Ueno H, Takahashi H, Shiba N, Ohki K, Yuza Y, Tomizawa D, Adachi S, Ogawa S, Hayashi Y
  • Organizer: 第62回日本小児血液・がん学会学術集会，福島（ Web）
• [Presentation] A bioadhesive barrier-forming lipid solution for oral mucositis of pediatric patients with chemotherapy or radiation therapy （小児の科学療法、放射線療法に伴う口腔粘膜炎への局所管理ハイドロゲル創傷被覆・保護剤の使用経験）． (2020)
  • Author(s): Tanoshima R, Iizuka A, Hayashi H, Shimosato Y, Sasaki K, Tsujimoto S, Takeuchi M, Ohashi N, Shiba N
  • Organizer: 第62回日本小児血液・がん学会学術集会，福島（ Web）
• [Presentation] Etoposide, Cytarabine and Mitoxantrone- or Fludarabine, Cytarabine and Granulocyte Colony-Stimulating Factor-Based Intensive Reinduction Chemotherapy Is Recommended for Children with Relapsed Acute Myeloid Leukemia: The Results from the Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG) AML-05R Study. (2020)
  • Author(s): Moritake H, Tanaka S, Miyamura T, Nakayama H, Shiba N, Shimada A, Terui K, Yuza Y, Koh K, Goto H, Kakuda H, Saito A M, Hasegawa D, Iwamoto S, Taga T, Adachi S, Tomizawa D
  • Organizer: The 62nd ASH Annual Meeting and Exposition (virtual)
  • Int'l Joint Research
• [Presentation] Comprehensive Genetic Analysis Revealed Myeloid/Natural Killer (NK) Cell Precursor Acute Leukemia As a Novel Distinctive Leukemia Entity. (2020)
  • Author(s): Nishimura A, Yokoyama K, Yamagishi C, Imamura T, Naruto T, Morio T, Tanaka Y, Kanai A, Matsui H, Higuchi N, Shiba N, Miki M, Kimura F, Nakayama H, Nakazawa Y, Taga T, Taki T, Adachi S, Manabe A, Koh K, Ishida Y, Tojo A, Takagi M. et.al.
  • Organizer: The 62nd ASH Annual Meeting and Exposition (virtual)
  • Int'l Joint Research
• [Presentation] The Detection of Minor Clones with Somatic KIT D8 16V Mutations Using Droplet Digital PCR in Pediatric De Novo AML: AML-05 Trial from the Japanese Pediatric Leukemia/ Lymphoma Study Group. (2019)
  • Author(s): Sasaki K, Uchiyama Y, Ikeda J, Yoshitomi M, Shimosato-Wada Y, Tokumasu M, Matsuo H, Yoshida K, Oki K, Yamato G, Hara Y, Kinoshita A, Tomizawa D, Taga T, Adachi S, Tawa A, Horibe K, Matsumoto N, Ito S, Hayashi Y, Shiba N
  • Organizer: 61st American Society of Hematology Annual Meeting
  • Int'l Joint Research
• [Presentation] Recurrent Gene Mutations in Peadiatric Patients with AML By Targeted Sequencing -the Jccg Study, JPLSG AML-05-. (2019)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Yoshida K, Hara Y, Shiraishi Y, Ohki K, Sotomatsu M, Arakawa H, Matsuo H, Shimada A, Taki T, Kiyokawa N, Tomizawa D, Horibe K, Miyano S, Taga T, Adachi S, Ogawa S, Hayashi Y
  • Organizer: 61st American Society of Hematology Annual Meeting
  • Int'l Joint Research
• [Presentation] Clinical Features of Pediatric Acute Myeloid Leukemia with TP53 and CDKN2A/2B copy Number Alterations. (2019)
  • Author(s): Hara Y, Taki T, Yamato G, Yoshida K, Shiozawa Y, Shiba N, Kaburagi T, Shiraishi Y, Ohki K, Kawamura M, Sotomatsu M, Arakawa H, Matsuo H, Shimada A, Toki T, Kiyokawa N, Tomizawa D, Tago T, Ito E, Horibe K, Miyano S, Ogawa S, Adachi S, Hayashi Y
  • Organizer: 61st American Society of Hematology Annual Meeting
  • Int'l Joint Research
• [Presentation] Significant Feature of DNA Methylation at Bivalent Promotor and Repressed Polycomb Regions in Padiatric AML-the Jccg Study, JPLSG AML-05-. (2019)
  • Author(s): Yamato G, Kawai T, Shiba N, Hara Y, Ohki K, Kaburagi T, Yoshida K, Shiraishi Y, Miyano S, Kiyokawa N, Tomizawa D, Shimada A, Sotomatsu M, Arakawa H, Adachi S, Tago T, Horibe K, Ogawa S, Hata K, Hayashi Y
  • Organizer: 61st American Society of Hematology Annual Meeting
  • Int'l Joint Research
• [Presentation] Pediatric AML patients with FLT3-ITD/NUP98-NSD1 have maintained long-term complete remission. (2019)
  • Author(s): Yoshitomi M，Shimosato Y，Sasaki K，Oya T，Takeuchi M，Shiba N，Ito S
  • Organizer: 81st Annual Meeting of the Japanese Society of Hematology
• [Presentation] Recurrent gene mutations in pediatric AML by targeted sequencing using a 343-gene custom panel. (2019)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Yoshida K, Hara Y, Shiraishi Y, Ohki K, Sotomatsu M, Arakawa H, Matsuo H, Shimada A, Taki T, Kiyokawa N, Tomizawa D, Horibe K, Miyano S, Adachi S, Taga T, Ogawa S, Hayashi Y
  • Organizer: 81st Annual Meeting of the Japanese Society of Hematology
• [Presentation] Comprehensive methylation analysis in pediatric AML patients -the JCCG, JPLSG AML-05 study-. (2019)
  • Author(s): Yamato G, Kawai T, Shiba N, Hara Y, Ohki K，Kaburagi T, Yoshida K，Shiraishi Y, Miyano S, Kiyokawa N, Tomizawa D, Shimada A, Sotomatsu M, Arakawa H, Adachi S, Taga T，Horibe K, Ogawa S, Hata K, Hayashi Y
  • Organizer: 81st Annual Meeting of the Japanese Society of Hematology
• [Presentation] ATAC-seqを用いた小児急性骨髄性白血病におけるオープンクロマチン領域の解析． (2019)
  • Author(s): 池田順二，柴 徳生，飯塚淳広，吉富誠弘，佐々木康二，竹内正宣，鏑木多映子，大和玄季，原 勇介，林 泰秀，伊藤秀一
  • Organizer: 第61回日本小児血液・がん学会
• [Presentation] 小児急性骨髄性白血病におけるdroplet digital PCRを用いたKIT D816V変異を有するminor cloneの検出． (2019)
  • Author(s): 佐々木康二，柴 徳生，内山由理，徳舛麻友，松尾英将，吉田健一，大木健太郎，大和玄季，原 勇介，木下明俊，富澤大輔，多賀 崇，足立壮一，多和昭雄，堀部敬三，松本直通，伊藤秀一
  • Organizer: 第61回日本小児血液・がん学会
• [Presentation] 小児急性骨髄性白血病患者におけるKMT2C変異とPHF6変異の臨床的意義について． (2019)
  • Author(s): 鏑木多映子，大和玄季，柴 徳生，吉田健一，原 勇介，白石友一，大木健太郎，外松 学、荒川浩一，松尾英将，嶋田 明，滝 智彦，清河信敬，富澤大輔，堀部敬三，宮野 悟，足立壮一，多賀 崇，小川誠司，林 泰秀
  • Organizer: 第61回日本小児血液・がん学会
• [Presentation] TP53遺伝子異常を伴う小児急性骨髄性白血病の臨床像と分子生物学異常の解析． (2019)
  • Author(s): 原 勇介，滝 智彦，大和玄季，吉田健一，柴 徳生，鏑木多映子，白石友一，大木健太郎，川村眞智子，外松 学，荒川浩一，松尾英将，嶋田 明，土岐 力，清河信敬，富澤大輔，多賀 崇，伊藤悦朗，堀部敬三，宮野 悟，小川誠司，足立壮一，林 泰秀
  • Organizer: 第61回日本小児血液・がん学会