Search for corresponding antigens for a novel Crohn's disease-specific antibody
Project/Area Number |
19K08481
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53010:Gastroenterology-related
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Research Institution | Kurume University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山崎 博 久留米大学, 医学部, 助教 (20529565)
吉岡 慎一郎 久留米大学, 医学部, 助教 (90425190)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | ACP353 / TCP353 / クローン病 / バイオマーカー / AIEC / ACP35 / 対応抗原 / 自己抗体 |
Outline of Research at the Start |
現在、クローン病の診断を行うためには内視鏡検査が重要であるが、高侵襲のため、より簡便で侵襲の少ない診断法の確立が期待されている。 これまでに我々は、ACP353という自己抗体がクローン病の患者さんに特異的に増加していることを見出した。もしもACP353が認識している抗原がわかればクローン病の血液診断が可能となり、さらにクローン病の原因の解明や新たな治療法の開発に繋げることができる。 この研究では、ACP353が認識している抗原を決定し、それをもとにクローン病の血液診断の測定法を確立する。次に、ACP353が認識している抗原がクローン病の原因に関係しているかを検討する。
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Outline of Final Research Achievements |
The main epitope of the ACP353-compatible antigen is the C-terminal 5 amino acid residue (GLFPN). In this study, we found multiple sequences that responded more strongly to Crohn's disease (CD) specimens than ACP353, and the feature on these sequences was CXXGLFPN. In other words, the reactivity of ACP353 could be improved by using Cys as the N-terminal of the antigenic peptide. These sequences are derived from human, bacteria, and parasite proteins, which have not yet determined as ACP353-compatible antigens. Furthermore, the reactivity of Escherichia coli AIEC and ACP353, which may be suspected as one of the causative bacteria of CD, was confirmed by the western blot method, but no specific one could be identified. Further studies are needed to determine ACP353-compatible antigens.
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Academic Significance and Societal Importance of the Research Achievements |
もしACP353の対応抗原が同定できれば、ACP353よりも優れた感度と特異度を持つクローン病(CD)診断マーカーの測定系を構築することができ、その結果CDの診断が内視鏡を使用せずに血清のみで行える可能性がある。 さらにACP353の対応抗原がCDの病因に関わっていることが明らかになれば、原因不明とされるCDの病態解明のbreakthroughとなり、ひいては根治療法へとつながる可能性がある。
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Diagnostic accuracy of serum proteinase 3 antineutrophil cytoplasmic antibodies in children with ulcerative colitis2021
Author(s)
Tatsuki Mizuochi,Katsuhiro Arai,Takahiro Kudo,Ryusuke Nambu,Hitoshi Tajiri,Tomoki Aomatsu,Naoki Abe,Toshihiko Kakiuchi,Kunio Hashimoto,Tsuyoshi Sogo,Michiko Takahashi,Yuri Etani,Yugo Takaki,Ken-Ichiro Konishi,Jun Ishihara,Hitoshi Obara,Tatsuyuki Kakuma,Shunsuke Kurei,Yushiro Yamashita,Keiichi Mitsuyama
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Journal Title
Journal of Gastroenterology and Hepatology
Volume: 36
Issue: 6
Pages: 1538-1544
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Adrenomedullin for steroid-resistant ulcerative colitis: a randomized, double-blind, placebo-controlled phase-2a clinical trial.2021
Author(s)
Kita T, Ashizuka S, Ohmiya N, Yamamoto T, Kanai T, Motoya S, Hirai F, Nakase H, Moriyama T, Nakamura M, Suzuki Y, Kanmura S, Kobayashi T, Ohi H, Nozaki R, Mitsuyama K, Yamamoto S, Inatsu H, Watanabe K, Hibi T, Kitamura K.
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Journal Title
J Gastroenterol
Volume: 56
Issue: 2
Pages: 147-157
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Therapeutic Potential of a Self-Assembling Peptide Hydrogel to Treat Colonic Injuries Associated with Inflammatory Bowel Disease2021
Author(s)
Araki T, Mitsuyama K, Yamasaki H, Morita M, Tsuruta K, Mori A, Yoshimura T, Fukunaga S, Kuwaki K, Yoshioka S, Takedatsu H, Kakuma T, Akiba J, Torimura T.
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Journal Title
Journal of Crohn's and Colitis
Volume: -
Issue: 9
Pages: 1517-1527
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation of Serum Luecine-rich Alpha-2 Glycoprotein as a New Inflammatory Biomarker of Inflammatory Bowel Disease2021
Author(s)
Yoshimura T, Mitsuyama K, Sakemi R, Takedatsu H, Yoshioka S, Kuwaki K, Mori A, Fukunaga S, Araki Y, Morita M, Tsuruta K, Yamasaki H, Torimura T
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Journal Title
Mediators of Inflammation
Volume: 8825374
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Altered serum profile of the interleukin-22 system in inflammatory bowel disease2020
Author(s)
Sakemi R, Mitsuyama K, Morita M, Yoshioka S, Kuwaki K, Tokuyasu H, Fukunaga S, Mori A, Araki T, Yoshimura T, Yamasaki H, Tsuruta K, Morita T, Yamasaki S, Mizoguchi A, Sou S, Torimura T
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Journal Title
Cytokine
Volume: 136
Pages: 155264-155264
DOI
Related Report
Peer Reviewed
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[Journal Article] Highly bioavailable curcumin derivative ameliorates Crohn's disease symptoms: A randomized, double-blind, multicenter study2020
Author(s)
Sugimoto K, Ikeya K, Bamba S, Andoh A, Yamasaki H, Mitsuyama K, Nasuno M, Tanaka H, Matsuura A, Kato M, Ishida N, Tamura S, Takano R, Tani S, Osawa S, Nishihira J, Hanai H
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Journal Title
Journal of Crohns & Colitis
Volume: 14
Issue: 12
Pages: 1693-1701
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Safety and efficacy of single-needle leukocyte apheresis (LCAP) for treatment of ulcerative colitis2020
Author(s)
Shindo Y, Mitsuyama K, Yamasaki H, Imai T, Yoshioka S, Kuwaki K, Yamauchi R, Yoshimura T, Araki T, Morita M, Tsuruta K, Yamasaki S, Fukami K, Torimura T
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Journal Title
Therapeutic Apheresis and Dialysis
Volume: 24
Issue: 5
Pages: 503-510
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Gene Expression of Transient Receptor Potential Channels in Peripheral Blood Mononuclear Cells of Inflammatory Bowel Disease Patients2020
Author(s)
Morita T, Mitsuyama K, Yamasaki H, Mori A, Yoshimura T, Araki T, Morita M, Tsuruta K, Yamasaki S, Kuwaki K, Yoshioka S, Takedatsu H, Torimura T
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Journal Title
Journal of Clinical Medicine
Volume: 9
Issue: 8
Pages: 2643-2643
DOI
Related Report
Peer Reviewed
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[Journal Article] Antibodies to Crohn's Disease Peptide 353 as a Diagnostic Marker for Pediatric Crohn's Disease: A Prospective Multicenter Study in Japan2020
Author(s)
Mizuochi T, Arai K, Kudo T, Nambu R, Tajiri H, Aomatsu T, Abe N, Kakiuchi T, Hashimoto K, Sogo T, Takahashi M, Etani Y, Takaki Y, Konishi KI, Ishihara J, Obara H, Kakuma T, Kurei S, Yamashita Y, Mitsuyama K.
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Journal Title
Journal of Gastroenterology
Volume: 55
Issue: 5
Pages: 515-522
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation of Serum Calprotectin Levels in Patients with Inflammatory Bowel Disease2019
Author(s)
Atsushi Mori, Keiichi Mitsuyama, Ryosuke Sakemi, Shinichiro Yoshioka, Shuhei Fukunaga, Kotaro Kuwaki, Ryosuke Yamauchi, Toshihiro Araki, Tetsuhiro Yoshimura, Hiroshi Yamasaki, Kozo Tsuruta, Taku Morita, Sayo Yamasaki, Osamu Tsuruta, Takuji Torimura
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Journal Title
The Kurume Medical Journal
Volume: 66
Issue: 4
Pages: 209-215
DOI
NAID
ISSN
0023-5679, 1881-2090
Year and Date
2019-12-31
Related Report
Peer Reviewed / Open Access
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