• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The contribution of extracellular nucleotides and vesicles to the progression of atrial fibrillation

Research Project

Project/Area Number 19K08536
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53020:Cardiology-related
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Sasano Tetsuo  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00466898)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords心房細動 / 細胞外核酸 / 細胞外小胞 / cell-free DNA / バイオマーカ / バイオマーカー / セルフリーDNA / エクソソーム / ミトコンドリア / 心房リモデリング
Outline of Research at the Start

心房細動は最も頻度の高い持続性不整脈である。心房の組織的・電気的変化を特徴とする心房リモデリングは、心房細動発症および増悪の重要な要素である。また、心房細動は全身の炎症を伴い、脳梗塞・心不全などの全身性合併症を来すことがよく知られている。しかし、これらの病態のメカニズムはまだ完全に解明されていない。本研究では、心房リモデリングの新たなメカニズム、および心房細動と全身性合併症をつなぐメカニズムとして、細胞外核酸および細胞外小胞に着目し、その病態生理における寄与を解明する。さらに、これらが心房細動の発症予測や重症化予測、および全身性合併症予測のバイオマーカーとして有用か検証する。

Outline of Final Research Achievements

We investigated the intercellular communication mediated by extracellular nucleic acids and extracellular vesicles in atrial fibrillation (AF), and assess its relation to the progression of AF. When atrial myocytes had high -frequency electrical stimulation, specific extracellular vesicles were released into the culture medium, which induced an inflammatory response in adipocytes. We also demonstrated that this response was enhanced in the presence of macrophages.
Furthermore, we developed a method to directly quantify the particle size and particle number of extracellular vesicles in plasma without the process of vesicle extraction. Applying this method, we revealed an increase in extracellular vesicles in peripheral blood in arrhythmias such as atrial fibrillation and premature ventricular contractions. We also found that these vesicles induced distinct changes in target cells, such as vascular endothelial cells.

Academic Significance and Societal Importance of the Research Achievements

心房細動は本邦で診断されている患者数が90万人、発症しているが診断にはいたっていないと推定される患者数を合わせると約200万人になる。心房細動の最大の合併症は心原性脳塞栓であり、日本の寝たきりの約20%が心原性脳塞栓という報告もあることから、心房細動の病態の解明や早期介入への知見は非常に社会的意義の高いものといえる。
また本研究では、心房筋から放出される細胞外核酸や細胞外小胞に着目した研究であり、なぜ多くの不整脈疾患の中で心房細動のみが多彩な全身性合併症を生じるのか、という問いに答えるもので、学術的意義も高い。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (56 results)

All 2023 2022 2021 2020 2019 Other

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (38 results) (of which Int'l Joint Research: 6 results,  Invited: 15 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Extracellular Vesicles for the Treatment of Atrial Fibrillation.2023

    • Author(s)
      Ihara K and Sasano T.
    • Journal Title

      JACC Clin Electrophysiol.

      Volume: 9 Issue: 2 Pages: 159-160

    • DOI

      10.1016/j.jacep.2023.01.007

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seeking a Better Experimental Model of Atrial Fibrillation2022

    • Author(s)
      Ihara Kensuke、Sasano Tetsuo
    • Journal Title

      Circulation Journal

      Volume: 86 Issue: 2 Pages: 330-331

    • DOI

      10.1253/circj.CJ-21-0770

    • NAID

      130008144187

    • ISSN
      1346-9843, 1347-4820
    • Year and Date
      2022-01-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Local Injection of Hydroxyapatite Electret Ameliorated Infarct Size After Myocardial Infarction2022

    • Author(s)
      Yamaguchi Junji、Chiba Risako、Komuro Hiroaki、Ihara Kensuke、Nozaki Kosuke、Nagai Akiko、Furukawa Tetsushi、Sasano Tetsuo
    • Journal Title

      Circulation Reports

      Volume: 4 Issue: 1 Pages: 38-47

    • DOI

      10.1253/circrep.CR-21-0100

    • NAID

      130008140051

    • ISSN
      2434-0790
    • Year and Date
      2022-01-07
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene therapy to terminate tachyarrhythmias.2022

    • Author(s)
      Kawajiri K, Ihara K, Sasano T.
    • Journal Title

      Expert Rev Cardiovasc Ther.

      Volume: 20 Issue: 6 Pages: 431-442

    • DOI

      10.1080/14779072.2022.2085686

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Inflammation in the Pathogenesis of Atrial Fibrillation2022

    • Author(s)
      Ihara Kensuke、Sasano Tetsuo
    • Journal Title

      Frontiers in Physiology

      Volume: 13 Pages: 862164-862164

    • DOI

      10.3389/fphys.2022.862164

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of the recording condition on the quality of a single-lead electrocardiogram.2022

    • Author(s)
      Hamada S, Sasaki K, Kito H, Tooyama Y, Ihara K, Aoyagi E, Ichimura N, Tohda S, Sasano T.
    • Journal Title

      Heart Vessel.

      Volume: 37 Issue: 6 Pages: 1010-1026

    • DOI

      10.1007/s00380-021-01991-z

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sparsely methylated mitochondrial cell free DNA released from cardiomyocytes contributes to systemic inflammatory response accompanied by atrial fibrillation2021

    • Author(s)
      Yamazoe Masahiro、Sasano Tetsuo、Ihara Kensuke、Takahashi Kentaro、Nakamura Wakana、Takahashi Naomi、Komuro Hiroaki、Hamada Satomi、Furukawa Tetsushi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 5837-5837

    • DOI

      10.1038/s41598-021-85204-7

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional Role of the L396R Mutation of Tks5 Identified by an Exome-Wide Association Study in Atrial Fibrillation2020

    • Author(s)
      Yang Xiaoxi、Sasano Tetsuo、Ebana Yusuke、Takeuchi Jun K.、Ihara Kensuke、Yamazoe Masahiro、Furukawa Tetsushi
    • Journal Title

      Circulation Journal

      Volume: 84 Issue: 12 Pages: 2148-2157

    • DOI

      10.1253/circj.CJ-20-0101

    • NAID

      130007942473

    • ISSN
      1346-9843, 1347-4820
    • Year and Date
      2020-11-25
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Blood Coagulation Status during Cryofreezing Ablation and Effects of the Direct Anticoagulants Dabigatran and Edoxaban2020

    • Author(s)
      Okishige Kaoru、Hirao Tatsuhiko、Oda Atsushi、Shigeta Takatoshi、Nakamura Rena A.、Yoshida Hiroshi、Tachibana Shinichi、Yamauchi Yasuteru、Sasano Tetsuo、Hirao Kenzo
    • Journal Title

      International Heart Journal

      Volume: 61 Issue: 2 Pages: 249-253

    • DOI

      10.1536/ihj.19-450

    • NAID

      130007821507

    • ISSN
      1349-2365, 1349-3299
    • Year and Date
      2020-03-28
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice2020

    • Author(s)
      Ihara K, Sasano T, Hiraoka Y, Togo-Ohno M, Soejima Y, Sawabe M, Tsuchiya M, Ogawa H, Furukawa T, Kuroyanagi H
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 17894-17894

    • DOI

      10.1038/s41598-020-74800-8

    • NAID

      120007031666

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix2020

    • Author(s)
      Lee Jiyoung、Sutani Akito、Kaneko Rin、Takeuchi Jun、Sasano Tetsuo、Kohda Takashi、Ihara Kensuke、Takahashi Kentaro、Yamazoe Masahiro、Morio Tomohiro、Furukawa Tetsushi、Ishino Fumitoshi
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 4283-4283

    • DOI

      10.1038/s41467-020-18031-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system2020

    • Author(s)
      Komuro Hiroaki、Yamazoe Masahiro、Nozaki Kosuke、Nagai Akiko、Sasano Tetsuo
    • Journal Title

      Beilstein Journal of Nanotechnology

      Volume: 11 Pages: 1685-1692

    • DOI

      10.3762/bjnano.11.150

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An oriented hydroxyapatite film with arrayed plate‐like particles enhance chondrogenic differentiation of ATDC5 cells2019

    • Author(s)
      Komuro Hiroaki、Wint Wit Y.、Horiuchi Naohiro、Nozaki Kosuke、Sasano Tetsuo、Miyashin Michiyo、Yamashita Kimihiro、Nagai Akiko
    • Journal Title

      Journal of Biomedical Materials Research Part A

      Volume: 108 Issue: 3 Pages: 537-544

    • DOI

      10.1002/jbm.a.36834

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 心不全モデルマウスにおけるサクビトリル・バルサルタンの心房への抗不整脈効果2023

    • Author(s)
      岩宮賢、井原健介、古川哲史、笹野哲郎
    • Organizer
      心電学関連研究会2023
    • Related Report
      2022 Annual Research Report
  • [Presentation] レーザー回折散乱法による血漿中細胞外小胞の直接定量的測定2023

    • Author(s)
      新田義一、辰巳ことね、川尻紘平、井原健介、笹野哲郎
    • Organizer
      心電学関連研究会2021
    • Related Report
      2022 Annual Research Report
  • [Presentation] Exosomes in patients with atrial fibrillation may regulate adipocyte differentiation2023

    • Author(s)
      川尻紘平、ニョ ジン レイモン、井原健介、古川哲史、笹野哲郎
    • Organizer
      日本循環器学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 心房細動時に心房筋が心外膜脂肪組織に与える影響の解明2022

    • Author(s)
      石川紗佳、鈴木杏奈、岩嵜利奈、笹野哲郎
    • Organizer
      日本循環器学会関東甲信越地方会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ウェアラブル機器を用いた不整脈検出2022

    • Author(s)
      笹野哲郎
    • Organizer
      日本心臓病学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Risk stratification of atrial fibrillation and stroke from blood tests and novel ECG analysis2022

    • Author(s)
      Tetsuo Sasano
    • Organizer
      Asia pacific Heart rhythm society
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic and physiological factors to predict atrial fibrillation2022

    • Author(s)
      Tetsuo Sasano
    • Organizer
      日本不整脈心電学会学術大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 心房細動に対する新たなバイオマーカーと治療標的探索2022

    • Author(s)
      笹野哲郎
    • Organizer
      日本不整脈心電学会学術大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Application of artificial intelligence in the prediction and detection of atrial fibrillation2022

    • Author(s)
      Tetsuo Sasano
    • Organizer
      日本不整脈心電学会学術大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 心不全モデルのマウス心房に対するSacubitril/Valsartanの電気生理学的効果2022

    • Author(s)
      岩宮賢、井原健介、古川哲史、笹野哲郎
    • Organizer
      第85回日本循環器学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 心不全モデルマウスにおけるSacubitril/Valsartanの心房への電気生理学的効果2021

    • Author(s)
      岩宮賢、井原健介、古川哲史、笹野哲郎
    • Organizer
      心電学関連研究会2021
    • Related Report
      2021 Research-status Report
  • [Presentation] 心房細動モデルマウスのoptical mapping による評価2021

    • Author(s)
      岩宮賢、井原健介、富井直輝、山﨑正俊、黒柳秀人、佐久間一郎、古川哲史、笹野哲郎
    • Organizer
      心電学関連研究会2021
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 新規加算平均心電図とウェアラブル機器を用いた心房細動の早期検出・早期発見2021

    • Author(s)
      笹野哲郎
    • Organizer
      心電学関連研究会2021
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] AMED先進的医療機器・システム等技術開発事業 (基盤技術開発プロジェクト)「インテリジェント心房細動予防・検出インフラの構築」2021

    • Author(s)
      笹野哲郎
    • Organizer
      日本不整脈心電学会学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Application of AI on the prediction and detection of arrhythmias2021

    • Author(s)
      笹野哲郎
    • Organizer
      日本心臓病学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Involvement of mitochondrial cell-free DNA in systemic and local inflammatory response in atrial fibrillation.2021

    • Author(s)
      Tetsuo Sasano
    • Organizer
      Taiwan Heart Rhythm Society/TTS meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Prediction of AF by AI2021

    • Author(s)
      Tetsuo Sasano
    • Organizer
      Taiwan Heart Rhythm Society/TTS meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 心不全モデルマウスにおけるSacubitril/Valsartanの心房への電気生理学的効果2021

    • Author(s)
      岩宮賢、井原健介、古川哲史、笹野哲郎
    • Organizer
      日本不整脈心電学会心電学関連春季大会2021
    • Related Report
      2020 Research-status Report
  • [Presentation] 心房細動モデルマウスのoptical mapping による評価2021

    • Author(s)
      岩宮賢、井原健介、富井直輝、山﨑正俊、黒柳秀人、佐久間一郎、古川哲史、笹野哲郎
    • Organizer
      日本不整脈心電学会心電学関連春季大会2021
    • Related Report
      2020 Research-status Report
  • [Presentation] 新規加算平均心電図とウェアラブル機器を用いた心房細動の早期検出・早期発見2021

    • Author(s)
      笹野哲郎
    • Organizer
      日本不整脈心電学会心電学関連春季大会2021
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 心房細動のバイオマーカーとしての血中microRNAの安定性の評価2020

    • Author(s)
      濱田里美、近藤優里、佐々木奏絵、千葉里沙子、渡辺芙由子、市村直也、棗祐有、古川哲史、東田修二、笹野哲郎
    • Organizer
      第84回日本循環器学会
    • Related Report
      2020 Research-status Report
  • [Presentation] The possibility of relation between DNA methylation and atrial remodeling in pressure-overload murine model.2020

    • Author(s)
      Abe K, Sasano T, Fukayama H, Furukawa T
    • Organizer
      第84回日本循環器学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Novel Risk Stratification for Atrial Fibrillation Focusing on Atrial Remodeling.2020

    • Author(s)
      Sasano T, Yamazoe M, Ihara K, Mieda R, Kobayashi K, Hamamoto R, Furukawa T
    • Organizer
      第84回日本循環器学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Artificial Intelligence can identify patients with Atrial Fibrillation by evaluating 12 leads ECGs with sinus rhythm.2020

    • Author(s)
      Sasano T, Mieda R, Kobayashi K, Hamamoto R
    • Organizer
      第84回日本循環器学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 光学マッピング法による心不全モデルマウスにおける心房性不整脈の電気生理学的特性評価2020

    • Author(s)
      岩宮賢、井原健介、富井直輝、山﨑正俊、黒柳秀人、佐久間一郎、古川哲史、笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] 小型1ch心電計によるQRS波自動検出に必要な心電図条件の検討2020

    • Author(s)
      濱田里美、木藤穂高、佐々木奏絵、青柳栄子、市村直也、東田修二、笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] 肢誘導心電図のP波フラグメント解析による心房伝導障害の評価2020

    • Author(s)
      木藤穂高、佐々木奏絵、田中敏博、井原健介、古川哲史、笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] 深層学習を用いた、ウェアラブル脈波センサーによる心房細動の検出における体動ノイズの影響2020

    • Author(s)
      遠山優衣、佐々木奏絵、三枝凌太、木藤穂高、濱田里美、笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] 不整脈診療におけるAIの応用2020

    • Author(s)
      笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 遺伝子・バイオマーカーと新しい心電図解析による心房細動の早期発見2020

    • Author(s)
      笹野哲郎
    • Organizer
      心電学関連研究会2020
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] DNA methylation may be associated with atrial remodeling in pressure-overload murine model.2019

    • Author(s)
      Abe K, Sasano T, Fukayama H, Furukawa T
    • Organizer
      Asia-pacific Heart Rhythm Society meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] The Mechanism of cellular uptake of HAp nanoparticles for targeted gene delivery to cardiomyocytes2019

    • Author(s)
      Komuro H, Nozaki K, Yamazoe M, Furukawa T, Sasano T, Nagai A.
    • Organizer
      International Conference on Materials and Systems for Sustainability.
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Angiogenesis Therapy by Local Injection of Hydroxyapatite Electret in Murine Myocardial Infarction Model.2019

    • Author(s)
      Chiba R, Komuro H, Abe K, Yamazoe M, Ihara K, Soejima Y, Sawabe M, Furukawa T, Nagai A, Sasano T.
    • Organizer
      International Conference on Materials and Systems for Sustainability.
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Rare methylated cell-free DNA released from cardiomyocytes contributes to systemic inflammatory response accompanied with atrial fibrillation.2019

    • Author(s)
      Yamazoe M, Sasano T, Nakamura W, Ihara K, Takahashi N, Furukawa T.
    • Organizer
      第83回日本循環器学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Local injection of charged hydroxyapatite nanoparticle attenuates the myocardial injury in murine myocardial infarction model.2019

    • Author(s)
      千葉里沙子、山添正博、井原健介、小室博明、永井亜希子、古川哲史、笹野哲郎.
    • Organizer
      第83回日本循環器学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Intolerance to rapid ventricular pacing and coronary hypoplasty in pannexin-1 knockout mouse heart.2019

    • Author(s)
      井原健介、笹野哲郎、山添正博、古川哲史
    • Organizer
      第83回日本循環器学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Novel single nucleotide polymorphism identified in exome-wide association study contributes to atrial fibrillation via modulation of autonomic nervous function.2019

    • Author(s)
      Yamazoe M, Sasano T, Ihara K, Furukawa T.
    • Organizer
      第3回日本循環器学会基礎研究フォーラム
    • Related Report
      2019 Research-status Report
  • [Presentation] Involvement of DNA methylation in atrial remodeling in pressure-overload murine model.2019

    • Author(s)
      Abe K, Sasano T, Fukayama H, Furukawa T
    • Organizer
      第3回日本循環器学会基礎研究フォーラム
    • Related Report
      2019 Research-status Report
  • [Remarks] 東京医科歯科大学循環器内科研究紹介

    • URL

      https://tmd-cvm.jp/research-activities-2.html

    • Related Report
      2021 Research-status Report
  • [Remarks] 細胞外核酸・小胞を介した、不整脈における心臓と他臓器の臓器間コミュニケーション

    • URL

      https://tmd-cvm.jp/research-activities-2.html

    • Related Report
      2020 Research-status Report 2019 Research-status Report
  • [Remarks] 東京医科歯科大学プレスリリース:不整脈が全身の炎症を起こすメカニズムを解明

    • URL

      https://www.tmd.ac.jp/archive-tmdu/kouhou/20210318-3.pdf

    • Related Report
      2020 Research-status Report
  • [Patent(Industrial Property Rights)] 頻脈性不整脈検査方法および頻脈性不整脈検査システム2019

    • Inventor(s)
      笹野哲郎、山添正博、古川哲史
    • Industrial Property Rights Holder
      笹野哲郎、山添正博、古川哲史
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] 頻脈性不整脈検査方法および頻脈性不整脈検査システム2019

    • Inventor(s)
      笹野哲郎、山添正博、古川哲史
    • Industrial Property Rights Holder
      笹野哲郎、山添正博、古川哲史
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report
    • Overseas

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi