Development of novel liquid biopsy for viable-peritoneal tumor cells in peritoneal lavage fluid in pancreatic cancer
Project/Area Number |
19K09120
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 55020:Digestive surgery-related
|
Research Institution | Osaka University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
三善 英知 大阪大学, 医学系研究科, 教授 (20322183)
江口 英利 大阪大学, 医学系研究科, 教授 (90542118)
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Project Status |
Completed (Fiscal Year 2021)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 膵癌 / 腹腔内遊離癌細胞 / バイオマーカー / 微小循環癌細胞 / リキッドバイオプシー / ゲノム進化 / 癌不均一性 / テロメスキャン / リキッドバイオブシー / 糖鎖 |
Outline of Research at the Start |
切除可能(Resectable:R)-および切除可能境界(Borderline Resectable:BR)-膵癌患者を対象に癌切除標本を含め、新規liquid biopsy技術を応用し抗癌剤、放射線療法など実臨床の治療過程でのがん選択圧に暴露されたCTC, PTCをリアルタイムに検出・モニタリングしliquid clinical sequencingで包括的ゲノム解析を行い、がんの不均一性、ゲノム進化を重点的に探索し、ゲノム進化を加味した個別化医療の確立をめざす。
|
Outline of Final Research Achievements |
Positive peritoneal lavage cytology findings (CY+) in pancreatic cancer (PC) is defined as stage IV disease, however,the true value of CY+ for the patient’s prognosis remains controversial. The aim of this study was to evaluate use of a new modified telomerase-specific replication-selective adenovirus, expressing GFP (TelomeScan F35) in rapid detection of viable peritoneal tumor cell (v-PTC) dissemination of PC. If the presence of virally-detected, v-PTC predict peritoneal recurrence and patient outcome. Peritoneal lavage fluid was harvested after a laparotomy in 53 patients with PC. 2 patients were CY+ and v-PTC+, postoperative peritoneal recurrence early occurred. 4 were CY+, but v-PTC-, and no recurrence in the abdominal cavity were observed. 10 were CY-, but v-PTC+, and 4 of 10 patients occurred peritoneal recurrence. In conclusion, the v-PTC detection may be an independent prognostic factor and had close association with peritoneal recurrence.
|
Academic Significance and Societal Importance of the Research Achievements |
テロメスキャンF35システムにより「生きたPTCのみ可視化し高感度に検出」、自在に解析できる。この検出法はわれわれオリジナルのLiquid biopsy技術である。この新しいLiquid biopsyを膵癌手術時の腹腔洗浄細胞診の診断に応用し膵癌由来の生きたPTCを治療経過を通してリアルタイムモニタリングし、腹膜再発など予後および治療効果判定マーカーとしての臨床的意義を明らかにできた。 すなわち、進行膵癌では術前審査腹腔鏡によるconv-CYおよびTelo-CYの結果が膵切除実施の重要な因子となることが示唆された。現在、自動v-PTC検出システム確立を進めている。
|
Report
(4 results)
Research Products
(35 results)
-
[Journal Article] Randomized phase II study of gemcitabine and S-1 combination therapy versus gemcitabine and nanoparticle albumin-bound paclitaxel combination therapy as neoadjuvant chemotherapy for resectable/borderline resectable pancreatic ductal adenocarcinoma (PDAC-GS/GA-rP2, CSGO-HBP-015).2021
Author(s)
Yamada D, Kobayashi S, Takahashi H, Akita H, Yamada T, Asaoka T, Shimizu J, Takeda Y, Yokoyama S, Tsujie M, Tomokuni A, Tanemura M, Morimoto O, Murakami M, Kim Y, Nakahira S, Hama N, Sugimoto K, Hashimoto K, Doki Y, Eguchi H.
-
Journal Title
Trials
Volume: 22
Issue: 1
Pages: 568-568
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] A case of intraductal papillary mucinous neoplasm developing in the native pancreas after pancreas transplantation treated by total pancreatectomy.2021
Author(s)
Sato H, Tomimaru Y, Akita H, Kobayashi S, Ito T, Sasaki K, Iwagami Y, Yamada D, Noda T, Gotoh K, Takahashi H, Hama N, Asaoka T, Tanemura M, Doki Y, Eguchi H.
-
Journal Title
Clin J Gastroenterol.
Volume: 14
Issue: 6
Pages: 1766-1771
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Serum exosomal miR‐638 is a prognostic marker of HCC via downregulation of VE‐cadherin and ZO‐1 of endothelial cells2021
Author(s)
Yokota Y, Noda T, Okumura Y, Kobayashi S, Iwagami Y, Yamada D, Tomimaru Y, Akita H, Gotoh K, Takeda Y, Tanemura M, Murakami T, Umeshita K, Doki Y, Eguchi H.
-
Journal Title
Cancer Science
Volume: 112
Issue: 3
Pages: 1275-1288
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Clinical significance of preoperative CA19-9 and lymph node metastasis in intrahepatic cholangiocarcinoma.2020
Author(s)
Asaoka T, Kobayashi S, Hanaki T, Iwagami Y, Tomimaru Y, Akita H, Noda T, Gotoh K, Takeda Y, Tanemura M, Doki Y, Eguchi H.
-
Journal Title
Surg Today.
Volume: 50
Issue: 10
Pages: 1176-1186
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Optimal timing of laparoscopic cholecystectomy after gallbladder drainage for acute cholecystitis: A multi-institutional retrospective study.2020
Author(s)
Tomimaru Y, Fukuchi N, Yokoyama S, Mori T, Tanemura M, Sakai K, Takeda Y, Tsujie M, Yamada T, Miyamoto A, Hashimoto Y, Hatano H, Shimizu J, Sugimoto K, Kashiwazaki M, Kobayashi S, Doki Y, Eguchi H.
-
Journal Title
J Hepatobiliary Pancreat Sci.
Volume: 27
Issue: 8
Pages: 451-460
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Impact of CD36 on Chemoresistance in Pancreatic Ductal Adenocarcinoma.2020
Author(s)
Kubo M, Gotoh K, Eguchi H, Kobayashi S, Iwagami Y, Tomimaru Y, Akita H, Asaoka T, Noda T, Takeda Y, Tanemura M, Mori M, Doki Y.
-
Journal Title
Ann Surg Oncol.
Volume: 27
Pages: 610-619
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Heterogeneity of Treg/Th17 According to Cancer Progression and Modification in Biliary Tract Cancers via Self-Producing Cytokines.2020
Author(s)
Kinoshita M, Kobayashi S, Gotoh K, Kubo M, Hayashi K, Iwagami Y, Yamada D, Akita H, Noda T, Asaoka T, Takeda Y, Tanemura M, Eguchi H, Urakawa S, Goto K, Maekawa K, Wada H, Mori M, Doki Y.
-
Journal Title
Dig Dis Sci.
Volume: 65
Issue: 10
Pages: 2937-2948
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] The postoperative peak number of leukocytes after hepatectomy is a significant prognostic factor for cholangiocarcinoma.2019
Author(s)
Shinke G, Yamada D, Eguchi H, Iwagami Y, Akita H, Asaoka T, Noda T, Gotoh K, Kobayashi S, Takeda Y, Tanemura M, Doki Y, Mori M.
-
Journal Title
Mol Clin Oncol.
Volume: 10
Pages: 531-540
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] A case of hepatic pseudolymphoma in a patient with primary biliary cirrhosis.2019
Author(s)
Inoue M, Tanemura M, Yuba T, Miyamoto T, Yamaguchi M, Irei T, Seo S, Misumi T, Shimizu W, Suzuki T, Onoe T, Sudo T, Shimizu Y, Hinoi T, Tashiro H.
-
Journal Title
Clin Case Rep.
Volume: 7
Issue: 10
Pages: 1863-1869
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Exosomal miR-155 Derived from Hepatocellular Carcinoma Cells Under Hypoxia Promotes Angiogenesis in Endothelial Cells2019
Author(s)
Matsuura Y, Wada H, Eguchi H, Gotoh K, Kobayashi S, Kinoshita M, Kubo M, Hayashi K, Iwagami Y, Yamada D, Asaoka T, Noda T, Kawamoto K, Takeda Y, Tanemura M, Umeshita K, Doki Y, Mori M.
-
Journal Title
Dig Dis Sci
Volume: 64
Issue: 3
Pages: 792-802
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Clinical impact of viable circulating tumor cells (v-CTC) detection and PD-L1 expression on v-CTC in the patients with resectable pancreatic cancer2019
Author(s)
Masahiro Tanemura, Kenta Furukawa, Manabu Mikamori, Takurou Saito, Masahisa Otsuka, Yozo Suzuki, Kentaro Kishi, Hironao Yasuoka, Masahiko Tsujimoto, Yasuo Urata, Hiroki Akamatsu
Organizer
Annual Meeting 2019 American Association for Cancer Research
Related Report
Int'l Joint Research
-
-