• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new combined cancer immunotherapy for malignant soft tissue tumor

Research Project

Project/Area Number 19K09551
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56020:Orthopedics-related
Research InstitutionOkayama University

Principal Investigator

Nakata Eiji  岡山大学, 医歯薬学域, 講師 (10649304)

Co-Investigator(Kenkyū-buntansha) 宝田 剛志  岡山大学, 医歯薬学域, 教授 (30377428)
尾崎 敏文  岡山大学, 医歯薬学域, 教授 (40294459)
山田 大祐  岡山大学, 医歯薬学域, 助教 (50733680)
伊藤 達男  川崎医科大学, 医学部, 教授 (80789123)
上甲 良二  岡山大学, 大学病院, 医員 (80847856)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords骨肉腫 / 転移 / 浸潤 / PRX1 / 悪性骨・軟部腫瘍 / 肺転移 / 増殖 / PRRX1 / 増殖抑制 / シグナル伝達 / 悪性軟部腫瘍 / 複合がん免疫療法
Outline of Research at the Start

本研究の目的は、軟部肉腫に対する新しい複合がん免疫療法を確立すべく、PRX1阻害とニボルマブの抗がん作用をin vivo、in vitroで解明することである。PRX1阻害による抗がん剤作用を明らかにすることは、新規の抗がん剤療法の開発につながると考えている。さらに、PRX1阻害とニボルマブの併用は、肉腫に対する新規の複合がん免疫療法の開発につながる可能性がある。

Outline of Final Research Achievements

When PRRX1 was knocked down in an osteosarcoma cell line, which is a hyperlung metastatic strain, and the proliferative capacity was measured by the WST-8 assay, it was confirmed in vitro that the proliferation was strongly suppressed. It was also confirmed that when these cells were transplanted into mice, proliferation was suppressed as compared with the control group. In addition, when the osteosarcoma cells in which PRRX1 was knocked down were examined by wound healing assay and migration assay, the number of invading cells was reduced compared to the control group. Furthermore, osteosarcoma cells in which PRRX1 was knocked down were transplanted under the skin of mice, and 6 weeks later, the lungs of the mice were taken out and the number and size of lung metastases were compared with those of the control group. Then, it was found that the osteosarcoma cell group in which PRRX1 was knocked down had a smaller number of lung metastases.

Academic Significance and Societal Importance of the Research Achievements

骨肉腫は四肢に多く発生し、肺転移を起こす予後不良な悪性腫瘍である。有効な化学療法はほとんど開発されておらず、進行例に対する新規抗がん剤の開発が期待されている。我々の結果からPRRX1が骨肉腫の腫瘍細胞の増殖だけでなく、移動能・浸潤能に強く影響していることが明らかとなった。したがって、PRRX1は骨肉腫において、増殖および肺転移を促進している可能性を考えた。骨肉腫細胞株に対するPRX1阻害に関する研究はこれまでに報告がなく、我々の研究は、骨肉腫に対する新規治療の開発につながる、非常に重要な研究と考えている。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (5 results)

All 2021 2020

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] PRRX1 promotes malignant properties in human osteosarcoma2021

    • Author(s)
      Joko Ryoji、Yamada Daisuke、Nakamura Masahiro、Yoshida Aki、Takihira Shota、Takao Tomoka、Lu Ming、Sato Kohei、Ito Tatsuo、Kunisada Toshiyuki、Nakata Eiji、Ozaki Toshifumi、Takarada Takeshi
    • Journal Title

      Translational Oncology

      Volume: 14 Issue: 1 Pages: 100960-100960

    • DOI

      10.1016/j.tranon.2020.100960

    • NAID

      120007004820

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 悪性末梢神経鞘腫瘍におけるPRRX1の悪性化因子としての役割2021

    • Author(s)
      たき平 将太
    • Organizer
      日本整形外科学会基礎学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 肉腫における免疫療法2021

    • Author(s)
      たき平 将太
    • Organizer
      日本整形外科学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 骨肉腫におけるPRRX1の発現は悪性化に関与する2020

    • Author(s)
      上甲良二
    • Organizer
      第134回 中部日本整形外科災害外科学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] PRRX1 expression is involved in the malignant transformation of osteosarcoma2020

    • Author(s)
      上甲良二
    • Organizer
      Orthopaedic Research Society 2020 Annual Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi