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Diagnosis of kidney graft dysfunction using metabolome analusis

Research Project

Project/Area Number 19K09700
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56030:Urology-related
Research InstitutionTokyo Medical University

Principal Investigator

Iwamoto Hitoshi  東京医科大学, 医学部, 教授 (00338831)

Co-Investigator(Kenkyū-buntansha) 杉本 昌弘  東京医科大学, 医学部, 兼任教授 (30458963)
今野 理  東京医科大学, 医学部, 講師 (50385027)
上野 琢哉  東京医科大学, 医学部, 兼任講師 (00296469)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsメタボローム / 腎移植 / 拒絶反応 / リキッドバイオプシー / 移植腎機能障害」 / 急性拒絶反応 / メタボローム解析 / 抗体関連型拒絶反応
Outline of Research at the Start

腎移植の成績は向上しているが10年生着率は86.0%であり経時的に生着率は低下する。その原因の一つとして拒絶反応が挙げられる。その内、抗体関連型拒絶反応が移植腎の長期予後を妨げる疾患として近年注目されている。そこで本研究では①代謝物の網羅的なメタボローム解析を用いて血清学的診断、病理診断と共に相互補完的に診断の難しい抗体関連型拒絶反応の診断法を含む拒絶反応を始めとする移植腎機能障害の診断法を開発する。②低侵襲に採取できる体液を用いたメタボローム解析による拒絶反応の診断マーカーを探索・評価する事を目的とする。

Outline of Final Research Achievements

The early and accurate diagnosis of rejection after renal transplantation is required. The wide variety of novel biomarker discoveries using low-invasively available biofluids, such as blood and urea, has been conducted. Metabolomics is one of omics technologies to enable simultaneous identification and quantification of hundreds of metabolites. Our study showed the aberrance of 3-Indoxyl sulfate and trimethylamine N-oxide-related pathways, indicating the reflection of metabolic changes associated with kidney function.

Academic Significance and Societal Importance of the Research Achievements

低侵襲に採取できる体液は患者に負担をかけずに、高頻度な検査を実施できるため、学術的な視点でも、本研究は非常にユニークなものである。世界的にもさかんにこの分野すなわちリキッドバイオプシーの研究は行われているが、移植領域は癌領域に比べまだ遅れているのが現状である。臨床応用されている拒絶反応を診断するバイオマーカーはまだない。本研究で移植腎機能障害群で 3-Indoxyl sulfate and trimethylamine N-oxide-related pathwaysの異常を検出できており、これらの代謝産物で拒絶反応を診断できる可能性がある。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Acute exacerbation of immunoglobulin A nephropathy complicated by alveolar hemorrhage after coronavirus disease 2019 vaccination: A case report.2023

    • Author(s)
      Uchida Takahiro、Sakai Takashi、Hoshino Takahiro、Kojima Aki、Konno Osamu、Yamada Muneharu、Iwamoto Hitoshi、Oda Takashi
    • Journal Title

      Medicine

      Volume: 102 Issue: 46 Pages: e36091-e36091

    • DOI

      10.1097/md.0000000000036091

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolomic Profiling of Plasma, Urine, and Saliva of Kidney Transplantation Recipients2022

    • Author(s)
      Iwamoto Hitoshi、Okihara Masaaki、Akashi Isao、Kihara Yu、Konno Osamu、Kawachi Shigeyuki、Sunamura Makoto、Sugimoto Masahiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 22 Pages: 13938-13938

    • DOI

      10.3390/ijms232213938

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] メタボローム解析による腎移植急性拒絶反応の分子マーカーの探索2020

    • Author(s)
      岩本整
    • Journal Title

      Organ Biology

      Volume: 27 Pages: 125-131

    • NAID

      130007888685

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 移植医療におけるliquid biopsyの応用2020

    • Author(s)
      岩本整
    • Journal Title

      Organ Biology

      Volume: 27 Pages: 31-35

    • NAID

      130007804896

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Diagnosis of acute graft rejection after kidney transplantation using metabolomic profiling of plasma, urine and saliva.2023

    • Author(s)
      Hitoshi Iwamoto, Osamu Konnno, Masaaki Okihara, Isao Akashi, Yu Kihara, Takuya Ueno, Masahiro Sugimoto
    • Organizer
      American transplant congress 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diagnosis of acute graft rejection after kidney transplantation using metabolomic profiling of plasma, urine and saliva.2023

    • Author(s)
      Hitoshi Iwamoto, Osamu Konnno, Masaaki Okihara, Isao Akashi, Yu Kihara, Takuya Ueno
    • Organizer
      The 18th congress of asian society of transplantation
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of a quadruple immunosuppressant regimen including early add-on everolimus after kidney transplantation2023

    • Author(s)
      Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno
    • Organizer
      European Society for Organ Transplantation (ESOT) 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diagnosis Of Acute Graft Rejection After Kidney Transplantation Using Metabolomic Profiling Of Biofluids2023

    • Author(s)
      Hitoshi Iwamoto
    • Organizer
      American transplant congress
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 腎移植領域におけるリキッドバイオプシーメタボローム解析による腎移植後急性拒絶反応の診断2022

    • Author(s)
      岩本 整
    • Organizer
      臨床腎移植学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Development of diagnosis method of acute kidney rejection after transplantation using metabolome analysis by liquid biopsy approach2020

    • Author(s)
      Hitoshi Iwamoto
    • Organizer
      American Transplant Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2025-01-30  

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