• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Dynamic Analysis of the Transition from Human Immature Oocytes to Parthenogenetic Embryos and the Association with Individual Aging and Chromosomal Distribution Abnormalities

Research Project

Project/Area Number 19K09820
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56040:Obstetrics and gynecology-related
Research InstitutionAkita University

Principal Investigator

Shirasawa Hiromitsu  秋田大学, 医学部附属病院, 講師 (60598019)

Co-Investigator(Kenkyū-buntansha) 熊澤 由紀代  秋田大学, 医学部附属病院, 准教授 (70400504)
佐藤 亘  秋田大学, 医学部附属病院, 助教 (10726441)
高橋 和政  秋田大学, 医学部附属病院, 技術系スタッフ (60791910)
安西 実武貴  秋田大学, 医学部, 技術系補佐員 (70770444)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords体外成熟培養 / 単為発生 / ヒト配偶子 / 着床前胚発育 / タイムラプスモニタリング / 動的挙動 / 第1極体 / 卵子凍結 / 妊孕性温存 / 前核 / タイムラプス / in vitro maturation / ヒト胚 / kinetics / PGT-A / 胚盤胞 / 未成熟卵子 / ヒト卵子 / 異数性 / 染色体分離異常 / 次世代シーケンス
Outline of Research at the Start

本研究はヒト未成熟卵子から単為発生ヒト胚盤胞に至るまでの、体外成熟培養、卵子成熟、単為発生過程、細胞分裂過程という複数のステップにおいて、ヒト個体加齢性変化の影響をタイムラプスによる動的挙動をもとに探るものである。ヒト卵子を用いた研究は各ステップにおける部分的な検討にとどまっており、分子生物学的な細胞内機能、動的挙動、そして最終的な胚盤胞異数性形成に至る関連性を未成熟卵子から連続して検討した報告は存在しない。未成熟卵子を用いる生殖補助医療の必要性は増加しており、卵子老化に関する各ステップの知見を得ることで、生殖医療の臨床にフィードバック可能な卵選別や質評価につながる成果を挙げる事が可能となる。

Outline of Final Research Achievements

For the first time, it has been clarified that in mature oocytes obtained after in vitro maturation culture of human immature oocytes, there are features associated with pre-implantation embryonic development in the duration from the appearance to the disappearance of the pronucleus in the subsequent parthenogenetic stimulation process. This is different from the timing of the appearance and disappearance of the pronucleus after ICSI in general infertility treatment, and it also suggested a connection with the timing of the first polar body expression during the in vitro maturation process, and the time required from the first polar body expression to parthenogenetic stimulation.

There have been no reports to date that continuously analyze the dynamic behavior from in vitro maturation to pre-implantation embryonic development in parthenogenesis in human gametes and embryos. The results obtained are beneficial for examining age-related changes in human embryos.

Academic Significance and Societal Importance of the Research Achievements

ヒト個体加齢による卵子老化において、染色体分配異常の増加は不妊治療における成績低下の大きな理由である。一方で妊孕性温存における卵子凍結は近年需要が増す中、個体加齢に加えたin vitro agingの影響に関しては検討が不十分であった。
第1極体放出という核成熟と、細胞質成熟の時間的乖離が、単為発生後の前核の挙動にどう影響するかを検討し、胚発育との関連を評価した初めての研究であり、今後の卵子老化研究の一助となる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] P-346?First comparison of the egg freezing and storage cost between medical adaptation and social adaptation of Japan fertility hospitals and clinics based on institution's websites2022

    • Author(s)
      Shirasawa H、Kumazawa Y、Sato W、Togashi K、Ono N、Fujishima A、Terada Y
    • Journal Title

      Human Reproduction

      Volume: 37 Issue: Supplement_1 Pages: 355-355

    • DOI

      10.1093/humrep/deac107.329

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 合併増大号 今月の臨床 生殖医療の基礎知識アップデート-患者説明に役立つ最新エビデンス・最新データ 一般不妊治療 特定の不妊原因に関する検査・治療 子宮内膜ポリープに対する治療2021

    • Author(s)
      白澤 弘光、熊澤 由紀代、寺田 幸弘
    • Journal Title

      臨床婦人科産科

      Volume: 75 Issue: 1 Pages: 94-99

    • DOI

      10.11477/mf.1409210231

    • ISSN
      0386-9865, 1882-1294
    • Year and Date
      2021-01-10
    • Related Report
      2021 Research-status Report
  • [Journal Article] A STUDY ON THE NECESSITY OF TIME LAPSE MONITORING FOR IN VITRO MATURATION IN COMBINED PROCEDURE AT THE TIMING OF OVARIAN TISSUE CRYOPRESERVATION2021

    • Author(s)
      Shirasawa Hiromitsu、Kumazawa Yukiyo、Sato Wataru、Takahashi Kazumasa、Togashi Kazue、Terada Yukihiro
    • Journal Title

      Fertility and Sterility

      Volume: 116 Issue: 3 Pages: e162-e162

    • DOI

      10.1016/j.fertnstert.2021.07.438

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Consistency between chromosomal status analysis of biopsied human blastocyst trophectoderm cells and whole blastocyst cells2021

    • Author(s)
      Takahashi Harunori、Takahashi Kazumasa、Goto Mayumi、Hirakawa Takeo、Hasegawa Hisataka、Shitara Akihiro、Iwasawa Takuya、Togashi Kazue、Makino Kenichi、Shirasawa Hiromitsu、Miura Hiroshi、Sato Wataru、Kumazawa Yukiyo、Terada Yukihiro
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 20 Issue: 4 Pages: 444-450

    • DOI

      10.1002/rmb2.12400

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell-free DNA in spent culture medium effectively reflects the chromosomal status of embryos following culturing beyond implantation compared to trophectoderm biopsy.2021

    • Author(s)
      Shitara A, Takahashi K, Goto M, Takahashi H, Iwasawa T, Onadera Y, Makino K, Miura H, Shirasawa H, Sato W, Kumazawa Y, Terada Y
    • Journal Title

      PLoS ONE

      Volume: 16 Issue: 2 Pages: e0246438-e0246438

    • DOI

      10.1371/journal.pone.0246438

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators2021

    • Author(s)
      Fujishima A, Takahashi K, Goto M, Hirakawa T, Iwasawa T, Togashi K, Maeda E, Shirasawa H, Miura H, Sato W, Kumazawa Y, Terada Y
    • Journal Title

      PLoS ONE

      Volume: 16 Issue: 1 Pages: e0246337-e0246337

    • DOI

      10.1371/journal.pone.0246337

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kinetics of meiotic maturation in oocytes from unstimulated ovaries and duration of pronucleus presence and preimplantation development2020

    • Author(s)
      Shirasawa Hiromitsu、Kumazawa Yukiyo、Takahashi Kazumasa、Goto Mayumi、Sato Wataru、Ono Natsuki、Togashi Kazue、Makino Kenichi、Waga Masato、Sato Naoki、Terada Yukihiro
    • Journal Title

      F&S Science

      Volume: 1 Issue: 2 Pages: 124-131

    • DOI

      10.1016/j.xfss.2020.09.004

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 第1極体放出からICSIまで20時間:卵巣組織凍結時の体外成熟培養(OTO-IVM)に おけるタイムラプス観察意義2022

    • Author(s)
      白澤弘光、熊澤由紀代、高橋和政、佐藤亘、尾野夏紀、後藤真由美、藤島綾香、寺田幸弘
    • Organizer
      第63回日本卵子学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 施設ホームページから得た医学的・社会的卵子凍結の実施有無 および凍結コストに関する比較検討2022

    • Author(s)
      白澤弘光 熊澤由紀代 佐藤亘 富樫嘉津恵 尾野夏紀 藤島綾香 寺田幸弘
    • Organizer
      第12回日本がん・生殖医療学会学術講演会
    • Related Report
      2021 Research-status Report
  • [Presentation] A STUDY ON THE NECESSITY OF TIME LAPSE MONITORING FOR IN VITRO MATURATION IN COMBINED PROCEDURE AT THE TIMING OF OVARIAN TISSUE CRYOPRESERVATION2021

    • Author(s)
      Hiromitsu Shirasawa , Yukiyo Kumazawa , Wataru Sato , Kazumasa Takahashi, Ph , D , Kazue Togashi , Yukihiro Terada
    • Organizer
      ASRM 2021 Scientific congress & expo
    • Related Report
      2021 Research-status Report
  • [Presentation] 本邦におけるAYA世代を中心とした、がん生殖医療の実際とこれから2020

    • Author(s)
      白澤弘光
    • Organizer
      第61回日本卵子学会学術集会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 体外成熟培養から着床前胚発育過程におけるタイムラプスイメージング評価の意義2020

    • Author(s)
      白澤弘光
    • Organizer
      第65回日本生殖医学会学術講演会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Novel approach for collecting human immature oocytes for reproductive research by vaginal ovaries transportation 1 hour after major vessel clipping in laparoscopic surgery2020

    • Author(s)
      Hiromitsu Shirasawa, Yukiyo Kumazawa, Wataru Sato, Yukihiro Terada
    • Organizer
      第72回日本産科婦人科学会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] The timing of the release of the first polar body predicts the cleavage rate after parthenogenetic activation for human oocytes obtained by in vitro maturation2019

    • Author(s)
      Hiromitsu Shirasawa, Yukiyo Kumazawa, Wataru Sato, Kazumasa Shirasawa, Yukihiro Terada
    • Organizer
      American Society for Reproductive Medicine (ASRM) 2019 Scientific Congress and Expo
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi