Lipid profiles of retinal pigment epithelial cells and the mechanism of Drusen formation.
| Project/Area Number |
19K09991
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56060:Ophthalmology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
Ikeda Hanako 京都大学, 医学研究科, 特定准教授 (20372162)
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| Co-Investigator(Kenkyū-buntansha) |
大石 明生 京都大学, 医学研究科, 特定病院助教 (50572955)
岩井 祥子 京都大学, 医学研究科, 特定研究員 (00768905)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 黄斑変性 / ドルーゼン / iPS / 網膜色素上皮 / 脂質 / 加齢黄斑変性 / iPS細胞 |
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| Outline of Research at the Start |
本研究では、大きなソフトドルーゼンを多数持つAMD及びADRD患者から樹立したiPS細
胞由来のRPE細胞を用い、
１）患者・健常人iPS由来のRPE細胞内の脂質プロファイリングを解明
２）長期・ストレス負荷培養で加齢を模した状況下の脂質プロファイリングの変化を解明
３）各RPE細胞のドルーゼン形成能と脂質プロファイリングの関連を解明
し、ドルーゼンの形成メカニズム解明ならびに治療法開発の礎を築くことを目的とする。
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| Outline of Final Research Achievements |
iPS cells were established from patients with macular degeneration and healthy subjects and induced to differentiate into retinal pigment epithelial cells. There were no significant differences in morphology or expression of retinal pigment epithelial cell-specific genes. Phagocytosis was slightly decreased in disease-derived retinal pigment epithelium. Comprehensive lipid analysis revealed that the intracellular lipid composition of retinal pigment epithelial cells derived from patients differed significantly from that of retinal pigment epithelial cells derived from healthy individuals. In addition, culture on membranes showed that patient-derived retinal pigment epithelial cells formed Drusen-like deposits.
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| Academic Significance and Societal Importance of the Research Achievements |
現在日本において加齢黄斑変性は中途失明の原因の一つであり、患者数は増加の一途をたどっている。本研究にて、黄斑変性患者由来の網膜色素上皮細胞では、健常者由来の網膜色素上皮細胞を比較して、細胞内脂質の組成が大きく異なる事が明らかになった。詳細な検討により、ドルーゼン形成のメカニズムが詳細に解明されれば、発症予防治療法の開発につながり、学術的、また社会的な意義は非常に大きいと考えられる。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Genotype and Long-term Clinical Course of Bietti Crystalline Dystrophy in Korean and Japanese Patients.2021
Author(s)
Murakami Y, Koyanagi Y, Fukushima M, Yoshimura M, Fujiwara K, Akiyama M, Momozawa Y, Ueno S, Terasaki H, Oishi A, Miyata M, Ikeda H, Tsujikawa A, Mizobuchi K, Hayashi T, Fujinami K, Tsunoda K, Park JY, Han J, Kim M, Lee CS, Kim SJ, Park TK, Joo K, Woo SJ, Ikeda Y, Sonoda KH.
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Journal Title
Ophthalmol Retina
Volume: -
Issue: 12
Pages: 1269-1279
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Association between topical β-blocker use and asthma attacks in glaucoma patients with asthma: a cohort study using a claims database2021
Author(s)
Kido A, Miyake M, Akagi T, Ikeda HO, Kameda T, Suda K, Hasegawa T, Hiragi S, Yoshida S, Tsujikawa A, Tamura H, Kawakami K
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Journal Title
Graefes Arch Clin Exp Ophthalmol
Volume: 260
Issue: 1
Pages: 271-280
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Safety and Effectiveness of a Novel Neuroprotectant, KUS121, in Patients With Non-Arteritic Central Retinal Artery Occlusion: An Open-Label, Non-Randomized, First-In-Humans, Phase 1/2 Trial2020
Author(s)
Ikeda HO, Muraoka Y, Hata M, Sumi E, Ikeda T, Nakagawa T, Abe H, Tada H, Morita S, Kakizuka A, Yoshimura N, Tsujikawa A
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Journal Title
Plos ONE
Volume: 15
Issue: 2
Pages: e0229068-e0229068
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Anterior Segment Optical Coherence Tomography Angiography Imaging of Conjunctiva and Intrasclera in Treated Primary Open-Angle Glaucoma2019
Author(s)
Akagi T, Uji A, Okamoto Y, Suda K, Kameda T, Nakanishi H, Ikeda HO, Miyake M, Nakano E, Motozawa N, Tsujikawa A
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Journal Title
Am J Ophthalmol
Volume: 208
Pages: 313-322
DOI
Related Report
Peer Reviewed
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[Journal Article] Concentric Choriocapillaris Flow Deficits in Retinitis Pigmentosa Detected Using Wide-Angle Swept-Source Optical Coherence Tomography Angiography.2019
Author(s)
Miyata M, Oishi A, Hasegawa T, Oishi M, Numa S, Otsuka Y, Uji A, Kadomoto S, Hata M, Ikeda HO, Tsujikawa A.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 60
Issue: 4
Pages: 1044-1049
DOI
Related Report
Peer Reviewed / Open Access
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