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Geometric organization of collagen fibers in human dermis and deformation mechanism of the fibers underlying pliability of the skin

Research Project

Project/Area Number 19K10007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56070:Plastic and reconstructive surgery-related
Research InstitutionKyoto University

Principal Investigator

Saito Susumu  京都大学, 医学研究科, 准教授 (00450239)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsイメージング / コラーゲン / 弾性線維 / 皮膚 / ヒト / 三次元 / 結合組織 / コラーゲン線維 / 幾何学的構造 / 多光子顕微鏡 / 組織透明化 / 定量的解析 / 二軸伸展 / 高次構造 / 変形
Outline of Research at the Start

皮膚の柔軟性のおかげで、ヒトは自由に関節を動かすことができる。皮膚は表皮と真皮で構成され、真皮では無数のコラーゲン線維や弾性線維が複雑にからみあっている。線維性臓器の柔軟性は個々の線維の変形と線維が集合して作る大きな構造の変形の両方から生まれる。これまで個々の線維の変形を観察した研究はなく、コラーゲンと弾性線維がどのように相互作用するかは不明である。またコラーゲン線維網の構造に規則性があるのかどうかの結論はでていない。本研究ではこれらの課題を解決するため新しい顕微鏡観察法を開発する。

Outline of Final Research Achievements

The dermis is composed of a jelly-like matrix and fibres. The fiber components are mainly collagen fibers and elastic fibers. Collagen fibers give strength to the skin, and elastic fibers give the skin elasticity. So far, the principal investigator has visualized the three-dimensional structure of collagen fibers and elastic fibers by treating the dermis with a method of stretching the dermis in all directions and a method of making the tissue transparent and observing it with multiphoton microscopy. In this research project, we succeeded in visualizing the deformation of human dermis using the method developed. We applied stretching stress in one direction and all directions to the human dermis. Fiber orientation was analyzed for all the layers using a series of images. We succeeded in visualizing the change in 3D fiber orientation of the dermis.

Academic Significance and Societal Importance of the Research Achievements

体がスムーズに動くためには、皮膚がしなやかに伸び縮みしなければならない。この伸び縮みは真皮のコラーゲン線維や弾性線維が関与している。この健常な柔軟性は疾患や外傷で失われる。例えば、エーラス・ダンロス症候群では皮膚に異常な弾性が生じる。外傷後や熱傷後に瘢痕ケロイドが生じれば、皮膚は伸びなくなり、外傷に対して脆弱となる。人工真皮を用いて再生した皮膚は弾性が乏しく、健常な皮膚が再生できたとは言えない。つまり、真皮の伸展機構を明らかにすることは、疾病学、創傷治癒学、再生医学において極めて重要である。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2023 2021 2019

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Three-dimensional analysis of load-dependent changes in the orientation of dermal collagen fibers in human skin: A pilot study2023

    • Author(s)
      MC Munisso, S Saito, I Tsuge, N Morimoto
    • Journal Title

      Journal of the Mechanical Behavior of Biomedical Materials

      Volume: 138 Pages: 105585-105585

    • DOI

      10.1016/j.jmbbm.2022.105585

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soft tissue abnormalities in Wassel Type VI radial polydactyly: a detailed anatomical study2021

    • Author(s)
      S. Saito, I Tsuge, H Yamanaka, N Morimoto.
    • Journal Title

      Journal of Hand Surgery (Eurpean Volume)

      Volume: 46 Issue: 4 Pages: 352-359

    • DOI

      10.1177/1753193421990212

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interphalangeal joint deviation in floating-type radial polydactyly with malalignment of the flexor pollicis longus tendon: a case report2021

    • Author(s)
      S. Saito, N Morimoto.
    • Journal Title

      Journal of Hand Surgery (Eurpean Volume)

      Volume: - Issue: 5 Pages: 556-558

    • DOI

      10.1177/1753193420982209

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Combined multiphoton imaging and biaxial tissue extension for quantitative analysis of geometric fiber organization in human reticular dermis2019

    • Author(s)
      Maho Ueda, Susumu Saito, Teruasa Murata, Tomoko Hirano, Ryoma Bise, Kenji Kabashima, Shigehiko Suzuki
    • Journal Title

      Scientific Reports

      Volume: 23:9(1) Issue: 1 Pages: 10644-10644

    • DOI

      10.1038/s41598-019-47213-5

    • NAID

      120006708224

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 弾性線維の弾性を可視化する多光子イメージング手法の確立2021

    • Author(s)
      齊藤晋, マリアキアラムニッソ, 津下到, 森本尚樹
    • Organizer
      第30回日本形成外科学会基礎学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] 三次元/ダイナミックイメージングの基本と手外科研究への応用2021

    • Author(s)
      齊藤晋
    • Organizer
      第7回手の造形手術研究会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 生体イメージング新時代:光超音波による微小血管の三次元構造と生体内挙動の可視化2021

    • Author(s)
      齊藤晋, 津下到, 山中浩気, 森本尚樹
    • Organizer
      第64回日本手外科学会学術集会 ランチョンセミナー9『光超音波イメージング技術を用いた 微細な脈管構造描出と臨床応用』
    • Related Report
      2021 Research-status Report
  • [Presentation] Dynamic Multi-Photon Imaging Shows Differences in Collagen Fiber Deformability Between Human skin and Keloids2019

    • Author(s)
      Susumu Saito, Maho Ueda, Itaru Tsuge, Naoki, Morimoto
    • Organizer
      The 2nd Congress of The Asian Pacific Society for Scar Medicine with The 14th Japan Scar Workshop
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 二軸伸展法を用いた緻密交織線維性結合組織の構造解析2019

    • Author(s)
      齊藤 晋
    • Organizer
      第4回エラスチン・関連分子研究会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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