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Gel vaccine designed to co-stimulate both humoral and cellular immunity ideal for elderly vaccination

Research Project

Project/Area Number 19K10097
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57020:Oral pathobiological science-related
Research InstitutionNihon University

Principal Investigator

CUENO Marni  日本大学, 歯学部, 専修研究員 (20569967)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsワクチン / gingival vaccine / influenza A H3N2 / influenza B/Yamagata / gel vaccine / influenza / influenza A / influenza B / pneumonia / vaccine / elderly
Outline of Research at the Start

Research Activity 1: Virulence factor entry through the gingival crevice can affect the body systemically by altering immune-related and ageing-related biochemical networks.
Research Activity 2: Virulence factor entry through the gingival crevice can affect the brain and nerve cells in vivo.
Research Activity 3: Identifying target amino acid residues in the hemagglutinin protein that could affect structural evolution and viral infection among seasonal and pandemic influenza strains.
Research Activity 4: Vaccination design and antigen production strategies.

Outline of Final Research Achievements

We established the CNS demyelination biochemical network in order to determine potential protein markers associated with any possible harmful effects associated with GC vaccination since vaccination with high levels of antigen may impact nerves causing CNS demyelination. Lastly, we elucidated the optimized antigen concentration to induce an immune response. Throughout this study, we were able to found the following: (1) xanthan molecules encapsulating an antigen does not interfere with epitope exposure; (2) gel-encapsulation enhances antigen structural stability; (3)CNS demyelination is mostly affected at the WNT/beta-catenin pathways; and (4) gel-encapsulated antigens elicit antibody response at 100 microgram per mL.

Academic Significance and Societal Importance of the Research Achievements

加齢に伴い口腔内歯肉溝(GC)の拡大と脆弱がみられることから、歯肉溝内の歯周病原性細菌が歯肉粘膜に入り込み易くなり、侵入した細菌が全身に影響をもたらす可能性が高い。これまでの研究で、GCは細菌の侵入経路であることを模倣し、ゲルカプセル化しGCに補給した単一の抗原が同経路で全身性免疫応答を引き起こす可能性を明らかにした。しかし、混合の抗原を用いて同様の実験を実施した場合に同等の全身性免疫応答を誘発するか否かは不明である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Structural Insights on the SARS-CoV-2 Variants of Concern Spike Glycoprotein: A Computational Study With Possible Clinical Implications2021

    • Author(s)
      Marni E. Cueno, Kenichi Imai
    • Journal Title

      Frontiers in Genetics

      Volume: 12 Pages: 773726-773726

    • DOI

      10.3389/fgene.2021.773726

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural Comparison of the SARS CoV 2 Spike Protein Relative to Other Human-Infecting Coronaviruses2021

    • Author(s)
      Cueno ME, Imai K
    • Journal Title

      Front Medicine

      Volume: 7 Pages: 594439-594439

    • DOI

      10.3389/fmed.2020.594439

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights on the structural variations of the furin-like cleavage site found among the December, 2019-July, 2020 SARS-CoV-2 spike glycoprotein: A computational study linking viral evolution and infection.2021

    • Author(s)
      Cueno ME, Ueno M, Iguchi R, Harada T, Miki Y, Yasumaru K, Kiso N, Wada K, Baba K, Imai K.
    • Journal Title

      Front Medicine

      Volume: 8 Pages: 613412-613412

    • DOI

      10.3389/fmed.2021.613412

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural insights into the potential changes in receptor binding site found in the influenza B Yamagata hemagglutinin: A putative correlation between receptor binding site structural variability and seasonal infection2020

    • Author(s)
      Marni E.Cueno, Kanako Iguchi, Kanta Suemitsu, Marina Hirano, Kosei Hanzawa, Takemasa Isoda, Miu Ueno, Rinako Iguchi, Aoi Otani, Kenichi Imai
    • Journal Title

      Journal of Molecular Graphics and Modelling

      Volume: 97 Pages: 107580-107580

    • DOI

      10.1016/j.jmgm.2020.107580

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Structural differentiation of the spike glycoprotein form SARS-CoV-2 COVID-19 variants of concern compared to other human-infecting coronaviruses: A computational study with clinical implications.2021

    • Author(s)
      Marni E. Cueno, Kenichi Imai
    • Organizer
      25th Congress of the Asia Pacific Society of Respirology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural variations in the influenza B hemagglutinin receptor binding site coincide with viral evolution and infection: A computational study with antiviral applications2019

    • Author(s)
      Marni E. Cueno, Kenichi Imai
    • Organizer
      27th Intelligent Systems for Molecular Biology and 18th European Conference on Computational Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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