Establishment of bacteriological examination method for diagnosing healing endodontic periodontal lesions

• Project/Area Number: 19K10124
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kobayashi Hiroaki 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (50396967)
• Co-Investigator(Kenkyū-buntansha): 川島 伸之 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (60272605) ; 竹内 康雄 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (60396968) ; 片桐 さやか 東京医科歯科大学, 歯学部附属病院, 准教授(キャリアアップ) (60510352) ; 須藤 毅顕 東京医科歯科大学, 統合教育機構, 特任助教 (10821168) ; 青木 章 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30302889)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯内歯周病変 / 歯周ポケット細菌 / 歯内歯周複合病変 / 細菌 / エンドペリオ

Outline of Research at the Start

エンドペリオ病変は、歯周ポケットと根尖病変が交通した疾患である。臨床的には、エンド病変から引き起こされたエンドペリオ病変は治癒しやすく、歯周炎から進展した病変は治癒しにくいといわれている。そこで、歯内病変から波及したエンドペリオ病変では、病変部にエンド由来の菌が多く、歯周病変由来の病変では、病変部にペリオの菌が多いという仮説を立て、少症例で解析したところ、術前に病変部歯周ポケットに歯周病由来の菌が多いエンドペリオ病変は「治癒しづらい」という傾向が得られた。本研究ではサンプル数を増やし、詳細に細菌叢を解析する。本研究の目的は治癒する歯内歯周病変を検査により判別できるようにすることである。

Outline of Final Research Achievements

Endoperio lesions are diseases in which periodontal pockets and apical lesions are in communication. Clinically, endoperio lesions caused by end lesions are easy to heal, and lesions that develop from periodontitis are difficult to heal. However, it is difficult to determine endoperio lesions caused by end lesions preoperatively. In this study, preoperative periodontal pocket bacteria were examined. As a result, endoperio lesions, which have a large amount of periodontal disease-derived bacteria in the periodontal pocket of the lesion before surgery, tend to be "difficult to heal." Because there are many endo-derived bacteria in the endoperio lesion that has spread from the endo lesion, it was suggested that bacterial tests could predict healing endoperio lesions.

Academic Significance and Societal Importance of the Research Achievements

根管治療で治癒するエンドペリオ病変を術前検査により判別できるようになれば、治療前にその病態にあった適切な治療方法が選択でき、いままでは治らないものと思われていた「エンドペリオ病変」の中で治癒しやすいものを判断できるようになり、安易な抜歯や、不要な歯周治療を防ぐことが可能となる。

Research Products

• [Journal Article] Corrigendum: Comparison of Periodontal Bacteria of Edo and Modern Periods Using Novel Diagnostic Approach for Periodontitis With Micro-CT (2022)
  • Author(s): Shiba Takahiko、Komatsu Keiji、Sudo Takeaki、Sawafuji Rikai、Saso Aiko、Ueda Shintaroh、Watanabe Takayasu、Nemoto Takashi、Kano Chihiro、Nagai Takahiko、Ohsugi Yujin、Katagiri Sayaka、Takeuchi Yasuo、Kobayashi Hiroaki、Iwata Takanori
  • Journal Title: Frontiers in Cellular and Infection Microbiology Volume: 12
  • DOI: 10.3389/fcimb.2022.871340
  • Peer Reviewed / Open Access
• [Journal Article] Prospective Longitudinal Changes in the Periodontal Inflamed Surface Area Following Active Periodontal Treatment for Chronic Periodontitis (2021)
  • Author(s): Nomura Y, Morozumi T*, Saito A, Yoshimura A, Kakuta E, Suzuki F, Nishimura F, Takai H, Kobayashi H, Noguchi K, Takahashi K, Tabeta K, Umeda M, Minabe M, Fukuda M, Sugano N, Hanada N, Yoshinari N, Sekino S, Takashiba S, Sato S, Nakamura T, Sugaya T, Nakayama Y, Ogata Y, Numabe Y, Nakagawa T
  • Journal Title: Journal of Clinical Medicine Volume: 10 Issue: 6 Pages: 1165-1165
  • DOI: 10.3390/jcm10061165
  • Peer Reviewed / Open Access
• [Journal Article] Potential Assessment of Dehydration during High-Intensity Training Using a Capacitance Sensor for Oral Mucosal Moisture: Evaluation of Elite Athletes in a Field-Based Survey (2021)
  • Author(s): Tanabe Gen、Hasunuma Tetsuya、Inai Yuto、Takeuchi Yasuo、Kobayashi Hiroaki、Hayashi Kairi、Shimizu Shintaro、Kamiya Nana S、Churei Hiroshi、Sumita Yuka I、Suzuki Katsuhiko、Moriya Naoki、Ueno Toshiaki
  • Journal Title: Chemosensors Volume: 9 Issue: 8 Pages: 196-196
  • DOI: 10.3390/chemosensors9080196
  • Peer Reviewed / Open Access
• [Journal Article] Estimation of the Periodontal Inflamed Surface Area by Simple Oral Examination (2021)
  • Author(s): Nomura Y, Morozumi T*, Numabe Y, Ogata Y, Nakayama Y, Sugaya T, Nakamura T, Sato S, Takashiba S, Sekino S, Yoshinari N, Hanada N, Sugano N, Fukuda M, Minabe M, Umeda M, Tabeta K, Takahashi K, Noguchi K, Kobayashi H, Takai H, Nishimura F, Suzuki F, Kakuta E, Yoshimura A, Saito A, Nakagawa T
  • Journal Title: Journal of Clinical Medicine Volume: 10 Issue: 4 Pages: 723-723
  • DOI: 10.3390/jcm10040723
  • Peer Reviewed / Open Access
• [Journal Article] Effective root planing for biologically acceptable root surfaces: an experimental study (2020)
  • Author(s): Kano Chihiro、Kobayashi Hiroaki、Nozaki Kosuke、Tsumanuma Yuka、Sudo Takeaki、Khemwong Thatawee、Mikami Ryo、Izumi Yuichi
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 62 Issue: 1 Pages: 1-15
  • DOI: 10.2329/perio.62.1
  • NAID: 130007821376
  • ISSN: 0385-0110, 1880-408X
  • Year and Date: 2020-03-31
  • Peer Reviewed / Open Access
• [Journal Article] A comparative questionnaire study of patient complaint levels between magnetostrictive ultrasonic scaler (Cavitron ? ) and piezoelectric ultrasonic scalers (2020)
  • Author(s): Ikeda Yuichi、Kawada Ayako、Tanaka Daiki、Ikeda Eri、Kobayashi Hiroaki、Iwata Takanori
  • Journal Title: International Journal of Dental Hygiene Volume: 00 Issue: 3 Pages: 1-6
  • DOI: 10.1111/idh.12478
  • Peer Reviewed / Open Access
• [Journal Article] Optimal Examination Sites for Periodontal Disease Evaluation: Applying the Item Response Theory Graded Response Model (2020)
  • Author(s): Nomura Y, Morozumi T*, Fukuda M, Hanada N, Kakuta E, Kobayashi H, Minabe M, Nakamura T, Nakayama Y, Nishimura F, Noguchi K, Numabe Y, Ogata Y, Saito A, Sato S, Sekino S, Sugano N, Sugaya T, Suzuki F, Takahashi K, Takai H, Takashiba S, Umeda M, Yoshie H, Yoshimura A, Yoshinari N, Nakagawa T
  • Journal Title: Journal of Clinical Medicine Volume: 9 Issue: 11 Pages: 3754-3754
  • DOI: 10.3390/jcm9113754
  • Peer Reviewed / Open Access
• [Journal Article] Unculturable and culturable periodontal-related bacteria are associated with periodontal inflammation during pregnancy and with preterm low birth weight delivery (2020)
  • Author(s): Ye Changchang、Xia Zhongyi、Tang Jing、Khemwong Thatawee、Kapila Yvonne、Kuraji Ryutaro、Huang Ping、Wu Yafei、Kobayashi Hiroaki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 15807-15807
  • DOI: 10.1038/s41598-020-72807-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Periodontopathic Bacteria in Placenta, Saliva and Subgingival Plaque of Threatened Preterm Labor and Preterm Low Birth Weight Cases: A Longitudinal Study in Japanese Pregnant Women (2020)
  • Author(s): Changchang Ye 1 , Sayaka Katagiri 2 , Naoyuki Miyasaka 3 , Hiroaki Kobayashi 4 , Thatawee Khemwong 2 , Toshiyuki Nagasawa 5 , Yuichi Izumi 2
  • Journal Title: Clin Oral Investig Volume: 24 Issue: 12 Pages: 4261-4270
  • DOI: 10.1007/s00784-020-03287-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical comparison of an electric‐powered ionic toothbrush and a manual toothbrush in plaque reduction: A randomized clinical trial (2020)
  • Author(s): Ikawa Takahiro、Mizutani Koji、Sudo Takeaki、Kano Chihiro、Ikeda Yuichi、Akizuki Tatsuya、Kobayashi Hiroaki、Izumi Yuichi、Iwata Takanori
  • Journal Title: International Journal of Dental Hygiene Volume: 19 Issue: 1 Pages: 93-98
  • DOI: 10.1111/idh.12475
  • Peer Reviewed / Open Access
• [Journal Article] The relationship between the anti-Porphyromonas gingivalis immunoglobulin G subclass antibody and small for gestational age delivery: a longitudinal study in pregnant Japanese women (2020)
  • Author(s): Changchang Ye, Hiroaki Kobayashi, Sayaka Katagiri, Naoyuki Miyasaka, Yasuo Takeuchi, Ryutaro Kuraji, Yuichi Izumi
  • Journal Title: Int Dent J Volume: 70 Pages: 296-302
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Salivary and serum interleukin-17A and interleukin-18 levels in patients with type 2 diabetes mellitus with and without periodontitis. (2019)
  • Author(s): Techatanawat S, Surarit R, Chairatvit K, Khovidhunkit W, Roytrakul S, Thanakun S, Kobayashi H, Khovidhunkit SP, Izumi Y.
  • Journal Title: PLoS ONE Volume: 15(2) Issue: 2 Pages: 0228921-0228921
  • DOI: 10.1371/journal.pone.0228921
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population (2019)
  • Author(s): Khemwong Thatawee, Kobayashi Hiroaki, Ikeda Yuichi, Matsuura Takanori, Sudo Takeaki, Kano Chihiro, Mikami Ryo, Izumi Yuichi.
  • Journal Title: Plos ONE Volume: 14(6) Issue: 6 Pages: e0218266-e0218266
  • DOI: 10.1371/journal.pone.0218266
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 治癒する歯周歯内病変を診断するための細菌学的検索 (2020)
  • Author(s): 小林 宏明, 川島 伸之, 須藤 毅顕, 加納 千博, 三神 亮, 片桐 さやか, 竹内 康雄, 岩田 隆紀
  • Organizer: 日本歯周病学会会誌