Elucidation of the mechanism of lung metastasis activation by lymph node dissection for oral cancer and development of the preventive methods
| Project/Area Number |
19K10303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森 士朗 東北大学, 大学病院, 講師 (80230069)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | リンパ節郭清 / リンパ節腫脹マウス / リンパ節転移モデルマウス / 肺微小転移 / 休眠細胞活性化 / 所属リンパ節郭清 |
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| Outline of Research at the Start |
本研究では、我々の研究グループが独自に樹立したリンパ節腫脹マウスを用いた肺微小転移休眠細胞活性化モデルマウスを用いて、 転移リンパ節(LN)郭清による肺転活性化の機序を血中循環腫瘍細胞および転移先臓器での前転移ニッチ形成部位の遺伝子発現の変動から明らかにし、それぞれの変動遺伝子群の上流遺伝子発現の制御を可能にする手法を見出し、肺転移予防法の開発を目指す。
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| Outline of Final Research Achievements |
Using a mouse model of lung metastasis, MXH10/Mo/lpr, in which micro-metastasis of the lung is activated by ablation of the lymph node (LN), a large amount of proteomic data were obtained by serum mass spectrometry analysis of molecules involved in the activation of lung metastasis with serum took before and after the activation. However, identification of the molecules involved has not been achieved. On the other hand, in the course of our study, it was found that doxorubicin-encapsulated liposomes (DOX-LP) injected into metastatic LNs and irradiated with ultrasound could inhibit the activation of the above-mentioned pulmonary micrometastases. We are currently investigating new strategies to elucidate the mechanism by which lung micrometastases are activated by resection of LNs in tumor-bearing mice and inactivated by intra-LN injection of DOX-LP and ultrasound irradiation.
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| Academic Significance and Societal Importance of the Research Achievements |
肺転移は頭頸部癌を含む多くの癌において生命予後を規定する極めて重要な因子であり、肺転移の予防法の開発は癌患者の治療成績の向上に極めて重要である。本研究では、担癌マウスのリンパ節(LN)の切除により肺転移が活性化する病因解明を血清質量分析法を用いて試みたが肺転移の活性化に関与する分子の同定には至らなかった。しかし、ドキソルビシン封入リポソーム(DOX-LP)を転移LNに注入し超音波照射することにより上記肺微小転移の活性化を抑制する新たな肺転移の病態解析モデルを樹立した。この研究モデルにより肺転移病巣の活性化抑制のための治療法の開発に新たな方向性を示すことができた。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Wound healing effect of autologous fibrin glue and polyglycolic acid sheets in a rat back skin defect model2021
Author(s)
Atsumu Kouketsu, Yoshinaka Shimizu, Shinnosuke Nogami, Minami Yamada-Fujiwara, Hirokazu Nagai, Kensuke Yamauchi, Hitoshi Miyashita, Haruka Saito, Kenji Odashima, Yuta Yanagisawa, Tetsu Takahashi
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Journal Title
Transfusion and Apheresis Science
Volume: 60
Issue: 4
Pages: 103144-103144
DOI
Related Report
Peer Reviewed
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[Journal Article] Changes in oral health-related quality of life after oral rehabilitation with dental implants in patients following mandibular tumor resection2019
Author(s)
Sato N, Koyama S, Mito T, Izumita K, Ishiko R, Yamauchi K, Miyashita H, Ogawa T, Kosaka M, Takahashi T, Sasaki K.
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Journal Title
Journal of Oral Science
Volume: 61
Issue: 3
Pages: 406-411
DOI
NAID
ISSN
1343-4934, 1880-4926
Related Report
Peer Reviewed / Open Access
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[Presentation] 3D models and simple approach using the mountable alignment guide for mandibular reconstruction with free fibular flap2020
Author(s)
Miyashita H, Sato N, Kitamura J, Takagi N, Kurosawa K, Kochi T, Ogawa T, Okoshi A, Izumida K, Takeda Y, Kataoka Y, Nogami S, Yamauchi K, Nagai H, Kato H, Koyama S, Takahashi T
Organizer
第38回日本口腔腫瘍学会
Related Report
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