What is the suitable chemical treatment for the next generation artificial titanium bone.

• Project/Area Number: 19K10348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Osaka Medical and Pharmaceutical University
• Principal Investigator: UENO TAKAAKI 大阪医科薬科大学, 医学部, 教授 (60252996)
• Co-Investigator(Kenkyū-buntansha): 山口 誠二 中部大学, 生命健康科学部, 准教授 (50726198) ; 眞野 隆充 (真野 隆充) 大阪医科薬科大学, 医学部, 准教授 (80325125)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2021: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: チタン / 顎再建 / フルカスタムデバイス / 積層造形 / 表面処理 / 混酸・加熱 / 骨再建 / 人工骨 / 表面性上 / 混酸・加熱処理 / 引き剥がし試験 / 組織学的観察 / 骨形成 / 血管新生 / フルカスタム / 擬似体液 / 積層造形チタン人工骨 / 骨再生

Outline of Research at the Start

積層造形技術は純チタン粒子からチタン製人工骨を自由な形に作り出すことを可能にする。この特性を医療機器作製へ応用することが期待されている。積層造形チタン人工骨が臨床応用されれば広範な骨欠損など硬組織再建において、腓骨・肩甲骨などの大きな骨ドナー採取が不要となり再建手術の侵襲軽減に大きく貢献できる。この申請課題では、申請者らがすでに実用化に向けて開発を進めているこの積層造形チタン人工骨表面に、混酸・加熱（＝H2SO4・HCL）処理を施す。この混酸・加熱処理の条件を変えて、チタン表面の微細構造ピット形状（＝多孔性）を変化させ、骨形成のための最適な混酸・加熱処理の最適化条件を解明する。

Outline of Final Research Achievements

We evaluated additive manufacturing titanium artificial bone(AMTAB) implanted into rat cranial bone defect. AMTAB will be introduced to cranio-maxillo-facial bone reconstruction without bending and adapting the fix plate.We also found the mixed-acid and heated treatment increase the potential of bone formation in vitro.However, this potential has not been clarified in vivo. In the present study, we implanted mixed-acid and heated AMTAB into the rat cranial bone defect and evaluated bone formation radiographically and histologically. Resulting, the mixed-acid and heated AMTA showed remarkable bone formation. The mixed-acid and heated treatment on the additive manufacturing titanium surface seemed to be effective in the animal body.

Academic Significance and Societal Importance of the Research Achievements

顎顔面領域の腫瘍や外傷で生じた骨欠損は患者の機能・審美障害を引き起こすため骨再建術は不可欠な治療法である。しかし、骨再建手術には移植骨として肩甲骨や腓骨から広範囲に組織を採取し欠損部に適合した形態に整える必要がある。この移植骨採取に伴う採取部位の二次的外科侵襲や採取骨量の限界、採取した骨を欠損部形態に適合させるための多大な労力などの解決が今後の再建医療の課題とされている。われわれはこの課題を解決するために、新たな医療マテリアルとして 3Dプリンター技術を応用したチタン粒子からなる積層造形技術を用い骨欠損部の形態に適合するカスタムメイド自由造形チタン人工骨の開発を行っている。

Research Products

• [Journal Article] Characterization of Salivary Microbiota in Patients with Atherosclerotic Cardiovascular Disease: A Case-Control Study (2022)
  • Author(s): Nahoko Kato-Kogoe, Shoichi Sakaguchi, Kuniyasu Kamiya, Michi Omori, Yan-Hong Gu, Yuri Ito, Shota Nakamura, Takashi Nakano, Junko Tamaki, Takaaki Ueno, Masaaki Hoshiga.
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 29 Issue: 3 Pages: 403-421
  • DOI: 10.5551/jat.60608
  • NAID: 130008165675
  • ISSN: 1340-3478, 1880-3873
  • Year and Date: 2022-03-01
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of salivary microbiota in elderly patients with type 2 diabetes mellitus: a matched case-control study. (2022)
  • Author(s): Michi Omori, Nahoko Kato-Kogoe, Shoichi Sakaguchi, Kuniyasu Kamiya, Nozomu Fukui, Yan-Hong Gu, Shota Nakamura, Takashi Nakano, Masaaki Hoshiga, Akihisa Imagawa, Chee Hoe Kit, Junko Tamaki, Takaaki Ueno.
  • Journal Title: Clin Oral Investig. Volume: 26 Issue: 1 Pages: 493-504
  • DOI: 10.1007/s00784-021-04027-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Investigation of Implant Stability Quotient Values of Dental Implants Placed in Vascularized Bone Grafts. (2022)
  • Author(s): Keisuke Matsumoto, Takamitsu Mano, Kazuya Inoue, Kayoko Yamamoto, Naoko Imagawa, Ayaka Takahashi and Takaaki Ueno.
  • Journal Title: Journal of Hard Tissue Biology Volume: 31 Pages: 55-58
  • NAID: 130008149075
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Histological Evaluation of Porous Additive-Manufacturing Titanium Artificial Bone in Rat Calvarial Bone Defects. (2021)
  • Author(s): Naoko Imagawa，Kazuya Inoue，Keisuke Matsumoto，Michi Omori，Kayoko Yamamoto, Yoichiro Nakajima，Nahoko Kato-Kogoe，Hiroyuki Nakano，Phuc Thi Minh Le，Seiji Yamaguchi，Takaaki Ueno．
  • Journal Title: Materials． Volume: 5360 Pages: 14-18
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The effect of simple heat treatment on apatite formation on grit-blasted/acid-etched dental Ti implants already in clinical use. (2021)
  • Author(s): Ayano Ogura, Seiji Yamaguchi, Phuc Thi Minh Le, Kayoko Yamamoto, Michi Omori, Kazuya Inoue, Nahoko Kato-Kogoe, Yoichiro Nakajima, Hiroyuki Nakano, Takaaki Ueno, Tomohiro Yamada and Yoshihide Mori.
  • Journal Title: Journal of Biomedical Materials Research: Part B - Applied Biomaterials. Volume: 110 Issue: 2 Pages: 392-402
  • DOI: 10.1002/jbm.b.34915
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] オスフェリオンDENTALの臨床使用. (2020)
  • Author(s): 井上和也, 中島世市郎, 中野旬之, 植野高章.
  • Journal Title: 日本歯科理工学会誌. Volume: 39 Pages: 29-32
  • Peer Reviewed
• [Journal Article] Relationship Between Oral Function and Occlusal Bite Force in the Elderly. (2020)
  • Author(s): Naoko Imagawa, Nahoko Kato-Kogoe, Kei Suzuki, Michi Omori, Kazuya Inoue, Hiroyuki Nakano, Kuniyasu Kamiya, Satoyo Ikehara, Masaaki Hoshiga, Junko Tamaki, Ryo Kawata, Takaaki Ueno.
  • Journal Title: Journal of Hard Tissue Biology. Volume: 29 Pages: 165-168
  • NAID: 130007877471
  • Peer Reviewed
• [Journal Article] Examination of Factor to Influence Dental Implant Stability Quotient Change. Journal of Hard Tissue Biology. 29(2): 131-134. 2020. (2020)
  • Author(s): Keisuke Matsumoto, Kazuya Inoue, Naoko Imagawa, Yoichiro Nakajima, Hiroyuki Nakano, Takaaki Ueno.
  • Journal Title: Journal of Hard Tissue Biology. Volume: 29 Pages: 131-134
  • Peer Reviewed
• [Journal Article] Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface. (2020)
  • Author(s): Naoko Imagawa, Kazuya Inoue, Keisuke Matsumoto, Ayako Ochi, Michi Omori, Kayoko Yamamoto, Yoichiro Nakajima, Nahoko Kato-Kogoe, Hiroyuki Nakano, Tomiharu Matsushita, Seiji Yamaguchi, Phuc Thi Minh Le, Shinpei Maruyama, Takaaki Ueno.
  • Journal Title: Materials. 2020; 13(22): e5104. Volume: 13
  • Peer Reviewed
• [Journal Article] Microsurgical mandibular reconstruction using a resin surgical guide combined with a metal reconstructive plate. (2019)
  • Author(s): Takashi Nuri, Koichi Ueda, Hiroyuki Iwanaga, Yuki Otsuki, Yoichiro Nakajima, Takaaki Ueno, Ryo Kawata.
  • Journal Title: Microsurgery Volume: 39(8) Issue: 8 Pages: 696-703
  • DOI: 10.1002/micr.30465
  • Peer Reviewed / Open Access
• [Journal Article] Examination of new parameters for sex determination of mandible using Japanese computer tomography data. (2019)
  • Author(s): Kei Suzuki, Hiroyuki Nakano, Kazuya Inoue, Yoichiro Nakajima, Sho Mizobuchi, Michi Omori, Nahoko Kato-Kogoe, Katsuaki Mishima, Takaaki Ueno. :
  • Journal Title: Dentomaxillofacial Radiology Volume: 2020 Pages: 41-47
  • Peer Reviewed / Open Access
• [Presentation] Evaluation of the stability of dental implants embedded in reconstructed bone using the Implant Stability Quotient. (2021)
  • Author(s): Kazuya Inoue, Hiroyuki Nakano, Kei Suzuki, Michi Omori, Naoko Imagawa, Kayoko Yamamoto, Nahoko Kato-Kogoe, Yoichiro Nakajima, Takaaki Ueno.
  • Organizer: The 25th Congress of the European Association for Cranio Maxillo Facial Surgery.
  • Int'l Joint Research
• [Presentation] Evaluation of bonding capability between the cortical bone and full custom-made titanium plate with mixed-acid and heat treatment surface modification. (2021)
  • Author(s): Naoko Imagawa, Kazuya Inoue, Kayoko Yamamoto, Kei Suzuki, Michi Omori, Yoichiro Nakajima, Hiroyuki Nakano, Nahoko Kato-Kogoe, Takaaki Ueno.
  • Organizer: The 25th Congress of the European Association for Cranio Maxillo Facial Surgery.
  • Int'l Joint Research
• [Presentation] Evaluation of titanium mesh sheet prepared by selective laser melting technique for alveolar ridge augmentation: clinical case reports. (2021)
  • Author(s): Kayoko Yamamoto, Nahoko Kato-Kogoe, Naoko Imagawa, Kazuya Inoue, Yoichiro Nakajima, Hiroyuki Nakano, Ian Matthew, Takaaki Ueno.
  • Organizer: The 25th Congress of the European Association for Cranio Maxillo Facial Surgery.
  • Int'l Joint Research
• [Presentation] 保険適応の広範囲支持装置 顎再建後の歯科インプラント (2021)
  • Author(s): 植野高章
  • Organizer: 第42回日本口腔インプラント学会中部支部学術大会 専門医教育講座
  • Invited
• [Presentation] 骨欠損部に適合する次世代フルカスタムデバイスの歯科インプラント骨移植への応用 (2021)
  • Author(s): 植野高章
  • Organizer: 第45回 国際口蓋裂学会
  • Invited
• [Presentation] 顎骨精密再建フルカスタムチタンプレートの臨床応用』産官学連携事業「健康寿命たかつきモデル」から見えた咬合再建の重要性 (2021)
  • Author(s): 植野高章
  • Organizer: 第31回日本口腔内科学会/第34回日本口腔診断学会
  • Invited
• [Presentation] 混酸・加熱処理を行った積層造形チタン人工骨の骨欠損部における組織学的観察． (2020)
  • Author(s): 1.今川尚子，井上和也，越智文子，松本佳輔，大森実知，小越菜保子，中野旬之，植野高章．
  • Organizer: 第74回NPO法人日本口腔科学会学術集会．
• [Presentation] SLMチタンメッシュプレートを用いた骨造成術の検討. (2020)
  • Author(s): 井上和也, 越智文子, 松本佳輔, 今川尚子, 大森実知, 小越菜保子, 中野旬之, 植野高章.
  • Organizer: 第74回NPO法人日本口腔科学会学術集会.
• [Presentation] Apatite formation of blast and acid-etched commercial dental Ti implant induced by heat treatment: effected of kinds of blast particles on apatite formation (2020)
  • Author(s): Ayano Ogura. Seiji Yamaguchi, Kayoko Yamamoto, Kazuya Inoue, Yoichiro Nakajima, Hiroyuki Nakano, Takaaki Ueno, Yoshihide Mori.
  • Organizer: 11th World Biomaterials Congress.
  • Int'l Joint Research
• [Presentation] チタン粉末より造形するフルカスタムデバイスの顎顔面インプラント治療への応用 (2020)
  • Author(s): 植野高章
  • Organizer: 第 24 回公益社団法人日本顎顔面インプラント学会総会・学術大会
  • Invited
• [Presentation] チタンと皮質骨の結合力評価試験装置の開発 (2019)
  • Author(s): 越智文子, 井上和也, 今川尚子, 松本佳輔, 小越菜保子, 中野旬之, 植野高章.
  • Organizer: 第28回硬組織再生生物学会学術大会・総会.
• [Presentation] 骨欠損部における多孔体積層造形チタン人工骨の組織学的観察 (2019)
  • Author(s): 今川尚子, 井上和也, 越智文子, 松本佳輔, 小越菜保子, 中野旬之, 植野高章.
  • Organizer: 第28回硬組織再生生物学会学術大会・総会.
• [Presentation] シェーグレン症候群患者へインプラント治療を行った1例. (2019)
  • Author(s): 田口尚吾, 植野高章, 砂野彰宏.
  • Organizer: 第49回公益社団法人日本口腔インプラント学会.
• [Presentation] 歯科インプラント安定指数(ISQ値)変化に影響を与える要素の検討. (2019)
  • Author(s): 松本佳輔, 井上和也, 中島世市郎, 山本佳代子, 今川尚子, 植野高章.
  • Organizer: 第49回公益社団法人日本口腔インプラント学会.
• [Presentation] Enhanced apatite formation of grit-blasted/acid-etched dental Ti implant by heat treatment. (2019)
  • Author(s): 小倉綾乃, 山口誠二, 今川尚子, 松本佳輔, 越智文子, 井上和也, 中島世市郎, 中野旬之, 植野高章, 森悦秀.
  • Organizer: PACRIM13 (The 13th Pacific Rim Conference of Ceramic Societies)
• [Book] 日本歯科理工学会誌. (2020)
  • Author(s): 井上和也, 中島世市郎, 中野旬之, 植野高章.
  • Total Pages: 139
  • Publisher: 一般社団法人 日本歯科理工学会