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Research on the changes of flavonoid content by cooking procedure and the antioxidant biomarkers in human after ingestion of flavonoid for utilizing food functionality.

Research Project

Project/Area Number 19K11645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 59040:Nutrition science and health science-related
Research InstitutionUniversity of Shizuoka

Principal Investigator

Ichikawa Yoko  静岡県立大学, 食品栄養科学部, 教授 (50269495)

Co-Investigator(Kenkyū-buntansha) 大槻 尚子  静岡県立大学, 食品栄養科学部, 助教 (20825004)
下位 香代子  静岡県立大学, 食品栄養科学部, 客員教授 (10162728)
合田 敏尚  静岡県立大学, 食品栄養科学部, 特任教授 (70195923)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsフラボノイド / 調理変化 / ヒト試験 / 生体内炎症指標 / 機能性活用 / 高フラボノイド食 / 食事 / 生体内炎症マーカー / 食事摂取 / 調理方法
Outline of Research at the Start

これまでに引き続き、フラボノイドの供給源となる食材について、各調理方法による調理後のフラボノイド含有量、総ポリフェノール量および抗酸化活性を測定、データを収集し、これを食品加工、食事の設計に活用できるよう整理する。また、食事(開発済みのフラボノイド低減食+フラボノイド含有量の多い食品1品)として摂取した際のフラボノイドの体内動態を摂取後9時間まで追い、傾向を明らかにする。さらに、高フラボノイド食の短期継続摂取による血中炎症マーカーへの影響を、酸化ストレス状態にあると想定される肥満の被験者への介入試験で明らかにする。

Outline of Final Research Achievements

For proffering flavonoid intake methods that can be used in food service business and home replacement meal, we measured changes in flavonoid content, antioxidant ability of foodstuffs by five different cooking methods using a steam convection oven, which is commonly used in commercial kitchens, and compared the results with those of general cooking methods. Flavonoid content significantly decreased in "boiled" cooking, while it significantly increased in "baked" and "steamed" tofu.
We also examined the effect of short-term continuous consumption of flavonoid-rich meals on inflammatory indices in a human study. In the obese group with BMI >25 kg/m2, blood flavonoid levels significantly increased and significantly decreased gene expression of inflammatory cytokines such as IL-1β in whole blood cells after 7 days of continuous intake. The results showed a significant decrease in gene expression of inflammatory cytokines such as IL-1β in whole blood cells.

Academic Significance and Societal Importance of the Research Achievements

食産業で導入率の高いスチームコンベクションオーブン調理による食材中のフラボノド含有量等の変化についてはほとんど報告がない。今後食品数を増やして検証し、データを蓄積することにより、中食、外食におけるヘルシーメニューへの応用等、食環境整備の上でも意義がある。
また、高フラボノイド含有量の食事の継続摂取では、肥満者において抗炎症作用が確認された。慢性的な炎症状態にある人に対しては、高フラボノイド食摂取が有効であること示された。さらに対象を増やして検証する必要があるが、本研究の結果は、生活習慣病予防のための食品機能性の食事への応用、食事の摂り方を提示するための有益な知見となりえる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2019

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Change of Flavonoid Contents Before and After Cooking in Model Menu of Daily Meals2022

    • Author(s)
      MANNEN Ryo, HANE Asako, SANO Ayami, OTSUKI Naoko, GODA Toshinao, SHIMOI Kayoko, ICHIKAWA Yoko
    • Journal Title

      Journal of Cookery Science of Japan

      Volume: 55 Issue: 2 Pages: 84-96

    • DOI

      10.11402/cookeryscience.55.84

    • ISSN
      1341-1535, 2186-5787
    • Year and Date
      2022-04-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本および諸外国におけるポリフェノール,フラボノイド摂取量の推定2021

    • Author(s)
      市川陽子, 萬年遼
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 59 Issue: 8 Pages: 408-415

    • DOI

      10.1271/kagakutoseibutsu.59.408

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2021-08-01
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of flavonoid-rich meals and low-flavonoid meals based on the dietary reference intakes for Japanese using basic foodstuffs on the gene expression of inflammatory cytokines in the whole blood cells from adult men of normal or light overweight2021

    • Author(s)
      Ryo Mannen, Michiko T. Yasuda, Ayami Sano, Toshinao Goda, Kayoko Shimoi, Yoko Ichikawa
    • Journal Title

      Functional Foods in Health and Disease

      Volume: 11(2) Issue: 2 Pages: 56-72

    • DOI

      10.31989/ffhd.v11i2.781

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in plasma concentration of flavonoids after ingestion of a flavonoid-rich meal prepared with basic foodstuffs2019

    • Author(s)
      Ryo Mannen, Michiko T. Yasuda, Ayami Sano, Toshinao Goda, Kayoko Shimoi, Yoko Ichikawa
    • Journal Title

      Functional Foods In Health And Disease

      Volume: 9(9) Issue: 9 Pages: 446-465

    • DOI

      10.31989/ffhd.v9i9.643

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 食材中のフラボノイド含有量、総ポリフェノール量、抗酸化活性に調理方法が及ぼす影響2022

    • Author(s)
      吉村夢那、萬年遼、大槻尚子、下位香代子、市川陽子
    • Organizer
      第75回日本栄養・食糧学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 玉ねぎ摂取時のケルセチンの体内動態に対する調理方法の検討2019

    • Author(s)
      鈴木杏奈、萬年遼、大原裕也、大槻尚子、下位香代子、市川陽子
    • Organizer
      第73回日本栄養・食糧学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 食材中の機能性成分に調理の種類および調理後の時間経過が及ぼす影響2019

    • Author(s)
      川崎杏香、萬年遼、大槻尚子、下位香代子、市川陽子
    • Organizer
      第73回日本栄養・食糧学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Effects of different cooking methods and storage time after cooking functional ingredients in foodstuffs2019

    • Author(s)
      Ryo Mannen, Momoka Kawasaki, Naoko Otsuki, Kayoko Shimoi, Yoko Ichikawa
    • Organizer
      The 9th International Conference on Polyphenols and Health
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 日本人の食事におけるフラボノイド摂取量と調理・保存によるフラボノイド・総ポリフェノール含有量、抗酸化活性の変動の検討2019

    • Author(s)
      市川陽子
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2019-04-18   Modified: 2023-01-30  

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