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Bioimaging and computational approach to elucidate genetic diseases based on phenotypic analysis

Research Project

Project/Area Number 19K12218
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 62010:Life, health and medical informatics-related
Research InstitutionEhime University

Principal Investigator

Saitou Takashi  愛媛大学, 医学系研究科, 講師 (60588705)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsバイオイメージング / 蛍光イメージング / 光学顕微鏡 / 画像解析 / メダカ / 遺伝統計学 / 統計解析
Outline of Research at the Start

疾患に関連する遺伝的多型の解析には,遺伝・環境因子と表現型との量的関係性を明らかにする必要があります.そのためにはモデル生物を利用した方法論の発展が重要です.本研究では,生きたまま個体全身に渡って器官特異的イメージングが可能なモデル動物であるメダカを利用し,系統間の表現型比較解析を可能とする画像解析技術との融合を図ることで,疾患に関わる遺伝背景の解明に向けた新規のアプローチを展開します.

Outline of Final Research Achievements

This study aimed to construct measurement and analysis techniques that enable phenotypic comparative analysis between medaka strains, which can image the whole body of individuals, in order to elucidate the genetic background related to diseases. First, we developed a wide-field, high-resolution two-photon excitation light sheet microscope for observing cells throughout the body of juvenile medaka fish. Using this microscope, we proceeded with the measurement and analysis of mutant strains with bone abnormalities, and devised a method for quantifying bone morphology using a local feature descriptor, and developed a method for comparing medaka mutant strains at the individual level.

Academic Significance and Societal Importance of the Research Achievements

遺伝学的な未解決問題をより基礎的な立場から検証するためにモデル生物を利用した計測・解析の方法論の発展は極めて重要である.メダカの利点は近交系の樹立が可能であることで遺伝学研究において脊椎動物としてユニークなモデルとなる.本研究では近年急速に発展している蛍光計測技術および画像解析技術の応用により定量的な個体解析から遺伝学へ結びつける研究を目的とした。今後これまで捉えられなかった表現型の検出から遺伝背景の理解が可能となると期待される.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (20 results)

All 2022 2021 2020 2019 Other

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination2021

    • Author(s)
      Takanezawa Sota、Saitou Takashi、Imamura Takeshi
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2979-2979

    • DOI

      10.1038/s41467-021-23249-y

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 次世代バイオイメージング実現のための技術開発2021

    • Author(s)
      今村健志,川上良介,齋藤卓
    • Journal Title

      OPTRONICS

      Volume: 480 Pages: 93-98

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 生きたままの生物を長期間観察できる2光子励起光シート顕微鏡の開発2021

    • Author(s)
      齋藤卓,高根沢聡太,今村健志
    • Journal Title

      実験医学

      Volume: 39 Pages: 3055-3058

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 生きたままの生物を長期間観察できる広視野・高分解能2光子励起ライトシート顕微鏡2021

    • Author(s)
      齋藤卓,高根沢聡太,今村健志
    • Journal Title

      光技術コンタクト

      Volume: 59 Pages: 38-44

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Donor-acceptor-acceptor-type near-infrared fluorophores that contain dithienophosphole oxide and boryl groups: Effect of the boryl group on the nonradiative deca2021

    • Author(s)
      Yoshiaki Sugihara, Naoto Inai, Thomas Baumgartner, Ryosuke Kawakami, Takashi Saitou, Takeshi Imamura, Masayasu Taki, Takeshi Yanai, Shigehiro Yamaguchi
    • Journal Title

      Chemical Science

      Volume: - Issue: 18 Pages: 6333-6341

    • DOI

      10.1039/d1sc00827g

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel regulatory mechanism against BMP/Smad signaling by Smurf2 in bone2020

    • Author(s)
      Junichi Kushioka, Takashi Kaito, Rintaro Okada, Hiroyuki Ishiguro, Zeynep Bal, Joe Kodama, Ryota Chijimatsu, Melanie Pye, Masahiro Narimatsu, Jeffrey Wrana, Yasumichi Inoue, Hiroko Ninomiya, Shin Yamamoto, Takashi Saitou, Hideki Yoshikawa, Takeshi Imamura
    • Journal Title

      Bone Research

      Volume: 8 Issue: 1 Pages: 41-41

    • DOI

      10.1038/s41413-020-00115-z

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment?colorectal cancer network2019

    • Author(s)
      Ieda Takeshi、Tazawa Hiroshi、Okabayashi Hiroki、Yano Shuya、Shigeyasu Kunitoshi、Kuroda Shinji、Ohara Toshiaki、Noma Kazuhiro、Kishimoto Hiroyuki、Nishizaki Masahiko、Kagawa Shunsuke、Shirakawa Yasuhiro、Saitou Takashi、Imamura Takeshi、Fujiwara Toshiyoshi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 16378-16378

    • DOI

      10.1038/s41598-019-52816-z

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 3D in vivo imaging of the keratin filament network in the mouse stratum granulosum reveals profilaggrin-dependent regulation of keratin bundling2019

    • Author(s)
      Usui Keiko、Kadono Nanako、Furuichi Yuki、Shiraga Keiichiro、Saitou Takashi、Kawasaki Hiroshi、Toyooka Kiminori、Tamura Hiroomi、Kubo Akiharu、Amagai Masayuki、Matsui Takeshi
    • Journal Title

      Journal of Dermatological Science

      Volume: 94 Issue: 3 Pages: 346-349

    • DOI

      10.1016/j.jdermsci.2019.04.006

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 広視野2光子励起ライトシート顕微鏡のライブイメージングへの応用2022

    • Author(s)
      齋藤卓,今村健志
    • Organizer
      レーザー学会学術講演会第42回年次大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 広視野型2光子励起ライトシート顕微鏡の開発2021

    • Author(s)
      齋藤卓,今村健志
    • Organizer
      第30回日本バイオイメージング学学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 2光子励起ライトシート顕微鏡の開発とin vivo細胞解析2021

    • Author(s)
      齋藤卓
    • Organizer
      第6回細胞ダイバース公開シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Wide Field Light-Sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination2021

    • Author(s)
      Takashi Saitou
    • Organizer
      Virtual TGFβ meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多光子励起自家蛍光スペクトルイメージングと機械学習を利用した肝線維化病態のデジタル診断2019

    • Author(s)
      齋藤卓,今村健志
    • Organizer
      第42回分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Whole body analysis of Medaka embryos with wide-field 2-photon light-sheet microscopy2019

    • Author(s)
      Takashi Saitou, Sota Takanezawa, Takeshi Imamura
    • Organizer
      Resonance Bio International Symposium 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Wide-field 2-photon light-sheet microscopy and its application to whole body imaging of medaka embryos2019

    • Author(s)
      Takashi Saitou, Takeshi Imamura
    • Organizer
      The 57th Annual Meeting of The Biophysical Society of Japan
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Wide-field 2-photon light-sheet microscopy and its application to cancer research using Medaka2019

    • Author(s)
      Takashi Saitou, Sota Takanezawa, Takeshi Imamura
    • Organizer
      The 78th Annual Meeting of the Japanese Cancer Association
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Whole body analysis of Medaka embryos using wide-field 2-photon light-sheet microscopy2019

    • Author(s)
      Takashi Saitou, Sota Takanezawa, Takeshi Imamura
    • Organizer
      The 25th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 広視野2光子ライトシート顕微鏡の開発とメダカ個体丸ごとイメージング2019

    • Author(s)
      齋藤卓,高根沢聡太,今村健志
    • Organizer
      第44回レーザ顕微鏡研究会
    • Related Report
      2019 Research-status Report
  • [Remarks] 生きたままの生物を長期間観察できる新しい顕微鏡技術の開発

    • URL

      https://www.ehime-u.ac.jp/data_relese/data_relese-160294/

    • Related Report
      2021 Annual Research Report
  • [Patent(Industrial Property Rights)] ライトシート顕微鏡用長距離伝搬ビーム形成レンズユニット及び長距離伝搬ビーム形成方法2019

    • Inventor(s)
      齋藤卓,高根沢聡太,今村健志
    • Industrial Property Rights Holder
      齋藤卓,高根沢聡太,今村健志
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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