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Algal metabolic change caused by substances derived from adherent coexisting microorganisms and its application to hydrosphere environmental purification

Research Project

Project/Area Number 19K12386
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionNihon University

Principal Investigator

KAKITA Hirotaka  日本大学, 文理学部, 教授 (40356754)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords海藻 / 水圏環境浄化 / 海洋資源 / 植物
Outline of Research at the Start

微生物由来物質を添加した海水で培養することによる非成熟性オゴノリ科海藻単藻培養株のタンパク質発現変動を電気泳動(SDS-PAGE等)あるいはLC-MS/MS等を用いて網羅的に解析し、微生物由来物質添加により活性化される代謝経路と酵素タンパク質等に関する情報(酵素タンパク質等の検出情報)を獲得する。獲得した情報を利用し、高成長量、高栄養塩吸収能を持つ海藻の成長に最適な培養条件を選定する。

Outline of Final Research Achievements

The purpose of this study is to clarify whether the biological functions of seaweeds suitable for hydrosphere environment purification are determined by seaweed species or related to the seaweed growing environment. Six strains of red algal family Gracilariaceae seaweeds with different habitats around the Seto Inland Sea in northeastern Shikoku in Japan were compared in growth rate, maturity, and hemagglutinin production. It was clarified that the biological functions of the same species differ depending on the growing water area. The purpose of this study is also to find out whether IAA, which is a microbial substance, influences the fluctuation of proteins that are expected to be related to seaweed growth. Extracts obtained from seaweeds grown in different IAA concentrations were analyzed by LC-MS/MS. The results of LC-MS / MS analysis showed that the concentration of IAA in the seawater medium affected the amount of biosynthesis of some enzymes in seaweed.

Academic Significance and Societal Importance of the Research Achievements

本研究では高成長率、非成熟性、有用物質高生産量等の水圏環境浄化に適した海藻の性質は海藻種内でも異なり、海藻生育水域の影響を受ける場合があることを明らかにした。このことは同一海藻種でも生育水域により生物機能に相違があることを明らかにした点で価値がある。この結果は、所望の生物機能を有する海藻株の獲得には天然水域生育海藻の幾つかの性質(例えば成長率や成熟性)の調査が有効であることも示唆している。またIAAが海藻の一部の酵素量に影響を及ぼしていることが示唆された。このことはIAA等の微生物由来物質により海藻の生産量や栄養塩吸収特性を変動できる可能性を示唆している点で意義がある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (22 results)

All 2022 2021 2020 2019 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (20 results) (of which Int'l Joint Research: 8 results) Remarks (1 results)

  • [Journal Article] Suitable unialgal strains of Gracilariopsis chorda and Gracilaria vermiculophylla for hemagglutinin production2020

    • Author(s)
      Hirotaka Kakita, Naoki Yanaoka, Hideki Obika
    • Journal Title

      Journal of Applied Phycology

      Volume: -

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 褐藻類ノコギリモクの抗アレルギー活性2022

    • Author(s)
      垣田浩孝、有本清也、田中守
    • Organizer
      2022年度日本農芸化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Effects of temperature and nitrogen chemical form on growth and hemagglutinin of the two red algae Gracilaria blodgettii and Gracilariopsis chorda from Japan2021

    • Author(s)
      Hirotaka Kakita, Seiya Arimoto, Hideki Obika
    • Organizer
      The 7th International Society for Applied Phycology Congress (ISAP2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of indole-3-acetic acid on proteins, carbohydrates and growth of the red alga, Gracilariopsis chorda from Japan2021

    • Author(s)
      Hirotaka Kakita, Naoki Yanaoka, Hideki Obika
    • Organizer
      The 7th International Society for Applied Phycology Congress (ISAP2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid and sensitive analysis of uronic acids derived from edible seaweeds2021

    • Author(s)
      Hirotaka Kakita, Shouhei Seki, Hideki Obika
    • Organizer
      The 7th International Society for Applied Phycology Congress (ISAP2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of light intensity on the growth and β-carotene biosynthesis of the green alga, Ulva prolifera2021

    • Author(s)
      Hirotaka Kakita, Tomoki Taniyama, Akinori Dan
    • Organizer
      3rd Seaweed for Health Conference 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Anti-allergic ingredients in the alcohol extracts derived from five kinds of brown algae in Japan2021

    • Author(s)
      Hirotaka Kakita, Seiya Arimoto, Hideki Obika
    • Organizer
      3rd Seaweed for Health Conference 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effects of water temperature on growth and nitrogen uptake of a red alga, Gracilaria blodgettii in Japan2021

    • Author(s)
      Hirotaka Kakita, Naoki Yanaoka, Chiaki Takashi, Ayaka Misawa, Hideki Obika
    • Organizer
      The Water and Environment Technology Conference Online 2021 (WET2021-online)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サンゴ粘液中の海藻忌避物質の探索2021

    • Author(s)
      垣田浩孝、高橋蘭、井口亮、鈴木淳
    • Organizer
      第73回日本生物工学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 鹿児島産オゴノリ科オゴノリとオゴノリ科ツルシラモの赤血球凝集活性画分の抽出と特徴付け2021

    • Author(s)
      垣田浩孝、柳岡直樹、寺田竜太
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 栄養塩と生活史がオゴノリ科海藻の赤血球凝集活性に及ぼす影響2021

    • Author(s)
      垣田浩孝、小比賀秀樹
    • Organizer
      日本農芸化学大会2021大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 緑藻類スジアオノリのβ-カロテン含有量と水温によるその変動2021

    • Author(s)
      谷山友規、團昭紀、垣田浩孝
    • Organizer
      日本農芸化学大会2021大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ウロン酸組成、タンパク質含量、アルギン酸関連酵素活性のコンブ部位比較2021

    • Author(s)
      平賀捷悟、川又澄人、四ツ倉典滋、垣田浩孝
    • Organizer
      日本農芸化学大会2021大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Dissolved nitrogen uptake of two red algae, Gracilariopsis chorda and Gracilaria bloegettii2020

    • Author(s)
      Hirotaka Kakita, Tomoki Taniyama, Ryouta Otoizumi, Hideki Obika
    • Organizer
      Water and Environment Technology Conference 2020 (WET2020)
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] 紅藻類オゴノリ科海藻の栄養塩吸収量及び成長に及ぼす水温とインドール3酢酸の影響2020

    • Author(s)
      垣田浩孝、有本清也、音泉良太、小比賀秀樹
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 緑藻類海藻スジアオノリの成長と成熟に対する水温および光強度の影響2020

    • Author(s)
      谷山友規、神山英行、團昭紀、垣田浩孝
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] コンブ部位によるアルギン酸含有量とタンパク質含有量の変動2020

    • Author(s)
      平賀捷悟、山田将也、関憧平、四ツ倉典滋、垣田浩孝
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 5種類の褐藻類大型海藻抽出液のヒアルロニダーゼ阻害活性2020

    • Author(s)
      有本清也、高崎隆平、垣田浩孝
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] オゴノリ科海藻の赤血球凝集活性に及ぼす培養水温の影響2020

    • Author(s)
      垣田浩孝、有本清也、音泉良太、三澤彩香
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Tke evaluation of a red alga, Gracilariopsis chorda as a biofilter2019

    • Author(s)
      Hirotaka Kakita, Hideki Obika, Yoshio Okuyama, Yoshiaki Takahashi
    • Organizer
      Water and Environment Technology Conference 2019 (WET2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 紅藻類オゴノリ科大型海藻のタンパク質含有量へのインドール3酢酸の影響2019

    • Author(s)
      垣田浩孝、小比賀秀樹
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Remarks] 日本大学文理学部化学科ホームページ

    • URL

      http://dep.chs.nihon-u.ac.jp/chemistry/

    • Related Report
      2021 Annual Research Report 2020 Research-status Report 2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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