Real-time three-dimensional temperature measurement of photothermal microbubbles by using quantum sensors
Project/Area Number |
19K15422
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 28050:Nano/micro-systems-related
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Research Institution | National Institutes for Quantum Science and Technology (2020-2021) Osaka City University (2019) |
Principal Investigator |
Nishimura Yushi 国立研究開発法人量子科学技術研究開発機構, 量子生命科学研究所, 博士研究員 (00824434)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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Keywords | 蛍光ナノダイヤモンド / ODMR / 温度 / 光検出磁気共鳴 / 温度計測 / ナノダイヤ / 対流 |
Outline of Research at the Start |
本研究は,光熱流体力学(フォトサーマルフルイディクス)における光熱バブル発生時に生じる熱対流の温度場を3次元的に可視化する計測手法の確立を行い,光熱効果による光濃縮メカニズムの解明を目的とする.温度によって蛍光強度が変化する蛍光ナノダイヤモンドを流体中に導入し,バブル周辺の流れ場の正確な温度分布イメージングを行う.これをマランゴニ効果として熱流体解析に取り入れ,バブル発生と流体制御の関係性に関する指導原理を与える.本研究は,食品や医療分野における微量物質検出技術への応用が期待されるフォトサーマルフルイディクスを確信的に進展させることが可能な挑戦的かつ独創的な研究課題である.
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Outline of Final Research Achievements |
We have developed a wide-field ODMR microscope that enables simultaneous temperature measurement at multiple points, and analyzed its characteristics in detail. The ODMR spectra obtained using confocal detection and widefield detection were compared with the same fluorescent nanodiamonds, we found that the ODMR is deeper with confocal detection than with widefield detection. The effects of pixel saturation and positional drift on wide-field ODMR detection were investigated to study measurement artifacts and sensitivity degradation. From this system characterization, we adjusted the measurement parameters and performed ODMR measurements of multiple fluorescent nanodiamonds in living HeLa cells.
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Academic Significance and Societal Importance of the Research Achievements |
本研究は,微小領域の高速・高精度な温度マッピングを可能にしようとするものである.共焦点検出とワイドフィールド検出はこれまで別々に扱われてきたが,1台の顕微鏡に組み込むことで初めて各々の特性を詳細に比較することができた.また,細胞内温度計測という観点においてはワイドフィールド検出の有用性を示すことができた.本研究成果は,カメラによる生きた細胞のリアルタイム大面積スピンベース測定の開発に役立つと期待される.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry2021
Author(s)
Yushi Nishimura, Keisuke Oshimi, Yumi Umehara, Yuka Kumon, Kazu Miyaji, Hiroshi Yukawa, Yutaka Shikano, Tsutomu Matsubara, Masazumi Fujiwara, Yoshinobu Baba, Yoshio Teki
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Journal Title
Scientific Reports
Volume: 11
Issue: 1
Pages: 4248-4248
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Real-time nanodiamond thermometry probing in vivo thermogenic responses2020
Author(s)
Masazumi Fujiwara, Simo Sun, Alexander Dohms, Yushi Nishimura, Ken Suto, Yuka Takezawa, Keisuke Oshimi, Li Zhao, Nikola Sadzak, Yumi Umehara, Yoshio Teki, Naoki Komatsu, Oliver Benson, Yutaka Shikano, Eriko Kage-Nakadai
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Journal Title
Science Advances
Volume: 6
Issue: 37
Pages: 9636-9636
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature2020
Author(s)
Hiroshi Yukawa, Masazumi Fujiwara, Kaori Kobayashi, Yuka Kumon, Kazu Miyaji, Yushi Nishimura, Keisuke Oshimi, Yumi Umehara, Yoshio Teki, Takayuki Iwasaki, Mutsuko Hatano, Hideki Hashimoto and Yoshinobu Baba
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Journal Title
Nanoscale Advances
Volume: 2
Issue: 5
Pages: 1859-1868
DOI
Related Report
Peer Reviewed / Open Access
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