Analysis of neurodegenerative mechanisms based on the development of beta amyloid oligomer-selective proteases

• Project/Area Number: 19K15694
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Tsukakoshi Kaori 東京農工大学, 工学(系)研究科(研究院), 助教 (20708474)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: アミロイドβ / アルツハイマー病 / プロテアーゼ / プリオンタンパク質 / オリゴマー

Outline of Research at the Start

アルツハイマー病の発症にはアミロイドβの凝集体の一種・アミロイドβオリゴマーが関与するとされている。アミロイドβオリゴマーは神経細胞内外の様々な部位に局在すると報告されているが、どの部位のアミロイドβオリゴマーが神経変性の原因であるかは不明である。そこで本研究ではアミロイドβオリゴマーを選択的に分解するプロテアーゼを開発し、これを用いて部位ごとにアミロイドβオリゴマーが与える神経変性機構を解析する。

Outline of Final Research Achievements

In this research project, a protease with amyloid-β (Aβ) oligomer selectivity, a drug target for Alzheimer's disease, was developed. Neprilysin was selected as the protease to degrade Aβ oligomer. As a result, recombinant production of Aβ oligomer-binding peptide-fused neprilysin with retained enzyme activity was successfully achieved. It was also found that the multivalency effect is important for the binding of peptides to Aβ oligomers. Future analysis of cells and tissues in which local Aβ oligomers are lowered by proteolysis is expected to reveal the action that triggers cell death.

Academic Significance and Societal Importance of the Research Achievements

Aβオリゴマーを標的とした二つの抗体医薬が上市され、認知機能低下を抑制する薬効が報告されている。しかし、今なおAβオリゴマーがどのように神経細胞を障害するのか、そのメカニズムは明らかでない。本研究課題の成果を基盤としそのメカニズムを解明することができれば、新しい創薬ターゲットの発見や高価な抗体医薬に変わる新薬の開発につながると期待できるため、その学術的・社会的意義は大きい。

Research Products

• [Journal Article] Identification of novel amyloidosis in dogs: α-S1-casein acquires amyloidogenicity in mammary tumor by overexpression and N-terminal truncation (2023)
  • Author(s): Murakami Tomoaki、Kaku Toshisuke、Tsukakoshi Kaori、Iwaide Susumu、Itoh Yoshiyuki、Hisada Miki、Nomura Kohji、Kubo Rikako、Ikebukuro Kazunori、Sassa-O’Brien Yukiko、Kametani Fuyuki
  • Journal Title: Veterinary Pathology Volume: 60 Issue: 2 Pages: 203-213
  • DOI: 10.1177/03009858221148511
  • Peer Reviewed / Open Access
• [Journal Article] Development of a Lateral Flow Assay for Bevacizumab Using an Anti-Idiotype DNA Aptamer as a Capture Molecule (2022)
  • Author(s): TODOROKI Kenichiro、ISOBE Ryota、YAMADA Tomohiro、SAITO Taro、SHIMIZU Yutaka、TSUKAKOSHI Kaori、SUGIYAMA Eiji、MIZUNO Hajime、HAYASHI Hideki、IKEBUKURO Kazunori
  • Journal Title: CHROMATOGRAPHY Volume: 43 Issue: 2 Pages: 73-77
  • DOI: 10.15583/jpchrom.2022.008
  • ISSN: 1342-8284, 1348-3315
  • Year and Date: 2022-06-20
  • Peer Reviewed / Open Access
• [Journal Article] Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides (2022)
  • Author(s): Anh Thi Tram Tu, Kazuaki Hoshi, Yue Ma, Taiji Oyama, Satoko Suzuki, Kaori Tsukakoshi, Kazuo Nagasawa, Kazunori Ikebukuro, Tomohiko Yamazaki
  • Journal Title: ACS Chem Biol Volume: 17 (7) Issue: 7 Pages: 1703-1713
  • DOI: 10.1021/acschembio.1c00904
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of Alkaline Phosphatase-Fused Mouse Prion Protein and Its Application in Toxic Aβ Oligomer Detection (2022)
  • Author(s): Kaori Tsukakoshi, Rikako Kubo, Kazunori Ikebukuro
  • Journal Title: Int J Mol Sci Volume: 23 (23) Issue: 23 Pages: 14588-14588
  • DOI: 10.3390/ijms232314588
  • Peer Reviewed / Open Access
• [Journal Article] The state of water molecules induces changes in the topologies and interactions of G-quadruplex DNA aptamers in hydrated ionic liquid (2022)
  • Author(s): Fujita Kyoko、Honda Takuya、Tsukakoshi Kaori、Ohno Hiroyuki、Ikebukuro Kazunori
  • Journal Title: Journal of Molecular Liquids Volume: 366 Pages: 120175-120175
  • DOI: 10.1016/j.molliq.2022.120175
  • Peer Reviewed / Open Access
• [Journal Article] CpG Methylation Altered the Stability and Structure of the i-Motifs Located in the CpG Islands (2022)
  • Author(s): Daiki Oshikawa, Shintaro Inaba, Yudai Kitagawa, Kaori Tsukakoshi, Kazunori Ikebukuro
  • Journal Title: Int J Mol Sci Volume: 23 (12) Issue: 12 Pages: 6467-6467
  • DOI: 10.3390/ijms23126467
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of DNAzymatic activity using iterative in silico maturation (2022)
  • Author(s): Renzo A Fenati, Zifei Chen, Yasuko Yamagishi, Kaori Tsukakoshi, Kazunori Ikebukuor, Anjay Manian, Salvy P Russo, Tomohiko Yamazaki, Amanda V Ellis
  • Journal Title: J Mater Chem B Volume: 10 (43) Issue: 43 Pages: 8960-8969
  • DOI: 10.1039/d2tb01638a
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection (2022)
  • Author(s): Saito Taro、Shimizu Yutaka、Tsukakoshi Kaori、Abe Koichi、Lee Jinhee、Ueno Kinuko、Asano Ryutaro、Jones Brian V.、Yamada Tomohiro、Nakano Tatsuki、Tong Jiaxing、Hishiki Asami、Hara Kodai、Hashimoto Hiroshi、Sode Koji、Toyo’oka Toshimasa、Todoroki Kenichiro、Ikebukuro Kazunori
  • Journal Title: Biosensors and Bioelectronics Volume: 203 Pages: 114027-114027
  • DOI: 10.1016/j.bios.2022.114027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cytotoxic Abeta Protofilaments Are Generated in the Process of Abeta Fibril Disaggregation. (2021)
  • Author(s): *Kaku, T., *Tsukakoshi, K. (*co-first author and corresponding author) & Ikebukuro, K.
  • Journal Title: Int J Mol Sci Volume: 22(23) Issue: 23 Pages: 12780-12780
  • DOI: 10.3390/ijms222312780
  • Peer Reviewed / Open Access
• [Journal Article] Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands (2021)
  • Author(s): Hasegawa Hijiri、Sasaki Ikkei、Tsukakoshi Kaori、Ma Yue、Nagasawa Kazuo、Numata Shusuke、Inoue Yuuki、Kim Yeji、Ikebukuro Kazunori
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 23 Pages: 13159-13159
  • DOI: 10.3390/ijms222313159
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparal-lel Guanine-Quadruplex Structural Scaffold. (2021)
  • Author(s): Safitri, F., Tu, A., Hoshi, K., Shobo, M., Zhao, D., Witarto, A., Sumarsono, S., Giri-Rachman, E., Tsukakoshi, K., Ikebukuro, K., and Yamazaki, T.
  • Journal Title: Sensors (Basel) Volume: 11(11) Issue: 11 Pages: 1617-1617
  • DOI: 10.3390/biom11111617
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol. (2021)
  • Author(s): *Tsukakoshi, K., *Yamagishi, Y. (*co-first author), Kanazashi, M., Nakama, K., Oshikawa, D., Savory, N., Matsugami, A., Hayashi, F., Lee, J., Saito, T., Sode, K., Khunathai, K., Kuno, H. & Ikebukuro, K.
  • Journal Title: Nucleic Acids Res Volume: 49 Issue: 11 Pages: 6069-6081
  • DOI: 10.1093/nar/gkab388
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins. (2021)
  • Author(s): Lee J, Teramoto K, Yokoyama T, Ueno K, Tsukakoshi K, Sode K, Ikebukuro K.
  • Journal Title: Data Brief Volume: 36 Pages: 107028-107028
  • DOI: 10.1016/j.dib.2021.107028
  • Peer Reviewed / Open Access
• [Journal Article] G-quadruplex: Flexible conformational changes by cations, pH, crowding and its applications to biosensing. (2021)
  • Author(s): Nishio M, Tsukakoshi K, Ikebukuro K
  • Journal Title: Biosens Bioelectron Volume: 178 Pages: 113030-113030
  • DOI: 10.1016/j.bios.2021.113030
  • Peer Reviewed / Open Access
• [Journal Article] A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria. (2020)
  • Author(s): Shono C, Ariyanti D, Abe K, Sakai Y, Sakamoto I, Tsukakoshi K, Sode K, Ikebukuro K.
  • Journal Title: Mar Biotechnol (NY) Volume: 23(1) Issue: 1 Pages: 31-38
  • DOI: 10.1007/s10126-020-09997-w
  • Peer Reviewed / Open Access
• [Journal Article] Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF (2020)
  • Author(s): Lee J, Tatsumi A, Tsukakoshi K, Wilson ED, Abe K, Sode K, Ikebukuro K.
  • Journal Title: Sensors (Basel) Volume: 20(14) Issue: 14 Pages: 3878-3878
  • DOI: 10.3390/s20143878
  • Peer Reviewed / Open Access
• [Journal Article] Generation of C5-desoxy analogs of tetrahydroisoquinoline alkaloids exhibiting potent DNA alkylating ability (2019)
  • Author(s): Tanifuji, Ryo; Tsukakoshi, Kaori; Ikebukuro, Kazunori; Oikawa, Hideaki; Oguri, Hiroki
  • Journal Title: Bioorganic & medicinal chemistry letters Volume: 29 (14) Issue: 14 Pages: 1807-1811
  • DOI: 10.1016/j.bmcl.2019.05.009
  • Peer Reviewed / Open Access
• [Journal Article] Ethanol Detection at the Parts per Billion Level with Single‐Stranded‐DNA‐Modified Graphene Field‐Effect Transistors (2019)
  • Author(s): Nozaki Ryo、Ikuta Takashi、Ueno Kinuko、Tsukakoshi Kaori、Ikebukuro Kazunori、Maehashi Kenzo
  • Journal Title: physica status solidi (b) Volume: 257 Issue: 2 Pages: 1900376-1900376
  • DOI: 10.1002/pssb.201900376
  • Peer Reviewed / Open Access
• [Journal Article] G-Quadruplex Structure Improves the Immunostimulatory Effects of CpG Oligonucleotides (2019)
  • Author(s): Hoshi, Kazuaki; Yamazaki, Tomohiko; Sugiyama, Yuuki; Tsukakoshi, Kaori; Tsugawa, Wakako; Sode, Koji; Ikebukuro, Kazunori
  • Journal Title: Nucleic acid therapeutics Volume: 29 (4) Issue: 4 Pages: 224-229
  • DOI: 10.1089/nat.2018.0761
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Rapid and convenient viral detection based on sequential enzymatic reactions induced by antibody-enzyme complex and aptamer binding (2022)
  • Author(s): Daimei Miura, Wakana Hayashi, Kensuke Hirano, Ikkei Sasaki, Kaori Tsukakoshi, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro, and Ryutaro Asano
  • Organizer: Active Enzyme Molecule 2022
  • Int'l Joint Research
• [Presentation] Exploration and application of DNA binding proteins as versatile tool for fabrication of biosensing elements (2022)
  • Author(s): Erika Komiya, Shouhei Takamatsu, Kaori Tsukakoshi, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro, and Ryutaro Asano
  • Organizer: Active Enzyme Molecule 2022
  • Int'l Joint Research
• [Presentation] Screening of aptamers specifically bind to various pollens (2022)
  • Author(s): Hatsune Tanaka, Yusuke Kadoguchi, Kaori Tsukakoshi, Kazunori Ikebukuro
  • Organizer: The 49th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Detection of CpG methylation based on structural change of G-quadruplex forming DNA oligonucleotide and its binding to heme protein (2022)
  • Author(s): Shintaro Inaba, Yudai Kitagawa, Kiwako Watanabe, Hijiri Hasegawa, Shusuke Numata, Kaori Tsukakoshi, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro
  • Organizer: The 49th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Proximity ligation assay using amyloid oligomer-binding aptamer with antibody indicates Aβ1-42 and Aβ3pE-42 oligomer formation in brain (2022)
  • Author(s): Kaori Tsukakoshi, Jo Kamada, Toshiya Ibaraki, Kazunori Ikebukuro
  • Organizer: The 49th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Anti-idiotype aptamer against Bevacizumab and its affinity change depending on pH (2022)
  • Author(s): Kazunori Ikebukuro, Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Ryutaro Asano, Tomohiro Yamada, Tatsuki Nakano, Kodai Hara, Hitoshi Hashimoto, Kenichiro Todoroki
  • Organizer: The 49th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] ループ改変によるαシヌクレインオリゴマー結合アプタマーの改良 (2022)
  • Author(s): 佐藤星, 塚越かおり, 池袋一典
  • Organizer: 第41回日本認知症学会学術集会
• [Presentation] アプタマー・抗体を用いたProximity ligation assayによる脳内Aβオリゴマーの検出 (2022)
  • Author(s): 塚越かおり, 茨木利哉, 鎌田晟, 池袋一典
  • Organizer: 第41回日本認知症学会学術集会
• [Presentation] タンパク質機能を制御するDNAアプタマーの開発：「アプタマー生物学」の創成へ (2022)
  • Author(s): 塚越 かおり, 小林 俊一, 守屋 里紗, 仲 和奏, 池袋 一典
  • Organizer: 第41回日本認知症学会学術集会
• [Presentation] Development of functional oligonucleotides forming G-quadruplex structure by using topological structure evaluation based on CD spectrum analysis combined with principal component analysis (2021)
  • Author(s): Kaori Tsukakoshi, Satoko Suzuki, Taiji Oyama, Akari Sato, Anh Thi Tram Tu, Yasuo Horiguchi, Koushi Nagamori, Tomohiko Yamazaki, Kazunori Ikebukuro
  • Organizer: 日本核酸医薬学会第6回年会
• [Presentation] グアニン四重鎖構造を用いたDNAアジュバントの開発 (2021)
  • Author(s): 山崎智彦, TU Thi Tram Anh, 星和明、Fika Ayu Safitri, 塚越かおり、池袋一典
  • Organizer: 日本核酸医薬学会第6回年会
• [Presentation] Evaluation of the aptamer binding to the target molecule in the mist using quartz crystal units for detection of virus in the air (2021)
  • Author(s): Ayaka Sone, Hatsune Tanaka, Kaori Tsukakoshi, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro
  • Organizer: 2021年電気化学秋季大会
• [Presentation] Evaluation of the structural effects of CpG methylation on the dopamine receptor gene(DRD2) (2021)
  • Author(s): Shinataro INABA, Yudai Kitagawa, Shiori Saito, Kaori Tsukakoshi, Wakako Tsugawa, Ryutaro Asano, Shunsuke Numata, Hijiri Hasegawa, Kazunori Ikebukuro
  • Organizer: 2021年電気化学秋季大会
• [Presentation] Analysis of inosine introduced aptamer against SARS-CoV-2 S1 protein RBD (2021)
  • Author(s): Wakana HAYASHI, Kaori TSUKAKOSHI, Kazunori IKEBUKURO
  • Organizer: 第15回バイオ関連化学シンポジウム
• [Presentation] Specific detection of a therapeutic antibody using an anti-idiotype DNA aptamer and drastic improvement of its affinity by pH optimization (2021)
  • Author(s): Yutaka Shimizu, Lisa Moriya, Taro Saito, Kaori Tsukakoshi, Koichi Abe, Jinhee Lee, Kinuko Ueno, Ryutaro Asano, Brian V. Jones, Tomohiro Yamada, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Koji Sode, Toshimasa Toyo’oka, Kenichiro Todoroki, and Kazunori Ikebukuro
  • Organizer: BIOSENSORS 2021
  • Int'l Joint Research
• [Presentation] Aptameric enzyme subunit enhances the peroxidase activity of myoglobin against luminol (2021)
  • Author(s): Kaori Tsukakoshi,Yasuko Yamagishi, Mana Kanazashi, Kenta Nakama, Daiki Oshikawa, Nasa Savory, Akimasa Matsugami, Fumiaki Hayashi, Jinhee Lee, Taiki Saito, Koji Sode, Kanjana Khunathai, Hitoshi Kuno, Kazunori Ikebukuro
  • Organizer: ISNAC2021
  • Int'l Joint Research
• [Presentation] Improvement of fluorescent aptamer focusing on its G-quadurplex and design of pH-sensitive Baby Spinach aptamer (2020)
  • Author(s): Kinuko UENO, Kaori TSUKAKOSHI, Alessandro PORCHETTA, Francesco RICCI, Kazunori IKEBUKURO
  • Organizer: 日本化学会第100春季年会
• [Presentation] Evaluation of structural features of α-S1-casein detected from amyloid deposits in canine mammary tumor (2020)
  • Author(s): Toshisuke Kaku, Rikako Kubo, Kaori Tsukakoshi, Tomoaki Murakami, Kazunori Ikebukuro
  • Organizer: 日本化学会第100春季年会
• [Presentation] 病態生理学的知見に基づいた毒性アミロイドβオリゴマー識別リガンドの開発 (2020)
  • Author(s): 塚越 かおり、久保 梨夏子、池袋 一典
  • Organizer: 第14回バイオ関連化学シンポジウム
• [Presentation] チャネル電流計測法を用いた脂質膜上におけるAβのポア構造形成過程の解析 (2020)
  • Author(s): 塚越 かおり、沼口 友理、清水 啓佑、川野 竜司、池袋 一典
  • Organizer: 第39回日本認知症学会学術集会
• [Presentation] 円二色性スペクトル解析に基づくαシヌクレインオリゴマー結合アプタマーの構造活性評価 (2019)
  • Author(s): 塚越 かおり, 鎌田 晟, 鈴木 仁子, 堀口 靖夫, 永森 浩司, 池袋 一典
  • Organizer: 日本核酸医薬学会第5回年会
• [Presentation] イヌの乳腺腫瘍随伴アミロイドーシスにおける前駆タンパク同定の試み(続報) (2019)
  • Author(s): 村上 智亮, 郭 俊佑, 久保 梨夏子, 塚越 かおり, 佐々 悠木子, 亀谷 富由樹
  • Organizer: 第七回日本アミロイドーシス学会学術集会
• [Presentation] ミオグロビン結合アプタマーによる, ミオグロビンのペルオキシダーゼ活性の増強 (2019)
  • Author(s): 中間健太, 山岸恭子, 金指真菜, 久野斉, 塚越かおり, 池袋一典
  • Organizer: 2019年電気化学秋季大会
• [Presentation] DNAアプタマーを用いたBevacizumabの検出法の開発 (2019)
  • Author(s): 清水裕, 齊藤太郎, 塚越かおり, 山田 朋宏, 轟木 堅一郎, 池袋 一典
  • Organizer: 2019年電気化学秋季大会
• [Presentation] プロモーター領域に存在する i-motif の安定性に CpG のメチル化が与える効果 (2019)
  • Author(s): 押川 大起, 塚越 かおり, 池袋 一典
  • Organizer: 第13回バイオ関連シンポジウム
• [Presentation] 蛍光発光アプタマーに対する三重鎖形成配列の付加による蛍光強度の増強 (2019)
  • Author(s): 上野 絹子, 塚越 かおり, Porchetta Alessandro, Ricci Francesco, 池袋 一典
  • Organizer: 第71回日本生物工学会大会
• [Presentation] Ligand-based functional improvement of G-quadruplex- forming DNA aptamers (2019)
  • Author(s): Kaori Tsukakoshi, Yuri Ikuta, Kaoru Konda, Ikkei Sasaki, Kazuo Nagasawa, Yoshio Kato, Chikashi Nakamura, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Improvement and design of pH-sensitive Baby Spinach aptamer by fusing triplex forming sequence (2019)
  • Author(s): Kinuko Ueno, Kaori Tsukakoshi, Alessandro Porchetta, Francesco Ricci, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Improvement in binding property of the anti-idiotype aptamer against bevacizumab based on structural information (2019)
  • Author(s): Yutaka Shimizu, Kaori Tsukakoshi, Ryutaro Asano, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Toshimasa Toyo’oka, Kenichiro Todoroki, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Effects of cation and G-quadruplex(G4) ligands on topology and binding ability of Cas9-binding aptamer (2019)
  • Author(s): Ikkei Sasaki, Maui Nishio, Kaori Tsukakoshi, Yue Ma, Kazuo Nagasawa, Ayana Yamagishi, Yoshio Kato, Chikashi Nakamura, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Enhancement of peroxidase activity of myoglobin by parallel G-quadruplex forming aptamer (2019)
  • Author(s): Kaori Tsukakoshi, Mana Kanazashi, Kenta Nakama, Akimasa Matsugami, Fumiaki Hayashi, Kanjana Khunathai, Hitoshi Kuno, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Topological structure evaluation of G-quadruplexes using high-throughput CD system (2019)
  • Author(s): Satoko Suzuki, Kaori Tsukakoshi, Taiji Oyama, Yasuo Horiguchi, Koushi Nagamori, and Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Evaluation of the effect of CpG methylation on i-motif structure located in the CpG islands (2019)
  • Author(s): Daiki Oshikawa, Kaori Tsukakoshi, Kazunori Ikebukuro
  • Organizer: ISNAC2019
  • Int'l Joint Research
• [Presentation] Aβ線維の脱凝集によるAβオリゴマー形成の検討 (2019)
  • Author(s): 郭 俊佑, 塚越 かおり, 池袋 一典
  • Organizer: 第３８回日本認知症学会学術集会
• [Presentation] Aβオリゴマーの検出に向けたアルカリホスファターゼ融合プリオンタンパク質の開発 (2019)
  • Author(s): 久保 梨夏子, 塚越 かおり, 李 鎭煕, 早出 広司, 池袋 一典
  • Organizer: 第３８回日本認知症学会学術集会
• [Presentation] アプタマーを用いたAβオリゴマーの組織染色法の検討 (2019)
  • Author(s): 塚越かおり, 鎌田晟, 細井千尋, 池袋一典
  • Organizer: 第３８回日本認知症学会学術集会
• [Patent(Industrial Property Rights)] 「核酸アプタマー、ヒト間葉系幹細胞吸着剤、ヒト間葉系幹細胞の分離方法、ヒト間葉系幹細胞の検出方法」 (2019)
  • Inventor(s): 池袋一典、三村かおり(結婚のため改姓)、西尾真初
  • Industrial Property Rights Holder: 池袋一典、三村かおり(結婚のため改姓)、西尾真初
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-146751
  • Filing Date: 2019