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Exploration of new antioxidant thiol biomolecules with valuable function by a new chemical analytical method of sulfur molecules

Research Project

Project/Area Number 19K15727
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Yusuke Kawano  筑波大学, 生命環境系, 助教 (40558029)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生体硫黄分子 / 硫黄代謝 / 硫黄 / 酸化還元
Outline of Research at the Start

本研究では、生体の酸化還元システムに深く関わり、全生物に必須の元素である「硫黄」に着目し、従来は困難とされる「“新規”の生体硫黄化合物、特に抗酸化性能を備えるチオール化合物(R-SH)の発見・同定とその特性解析」に挑む。その探索対象としては、光合成由来の「還元力(エネルギー)」を生産・供給する微生物の「藻類」が有望と考えた。当課題の推進には、一般的には困難な「硫黄代謝物種の一斉解析」を可能にしている「サルファーインデックス解析」をコア技術としてその優位性を活かす。以上、「硫黄生物学」の切り口から、「代謝機能」や「酸化還元システム」に対して、より拡張的で包括的な形の学術的理解の刷新を図る。

Outline of Final Research Achievements

In this study, I focused on "sulfur", which is deeply involved in the oxidation system of living organisms, and is then essential for all creatures. I here worked on the study, exploration, identification, and characterization of a "new" biological sulfur compound (especially with antioxidant effect), which has been conventionally difficult. The target organism was algae (Euglena), a microorganism that produces and supplies "reductant (energy)" derived from photosynthesis as a "producer" of the global life system. The promotion of this study was based on the "Sulfur Index Analysis", which enables the most difficult "simultaneous analysis of sulfur metabolites". As a result, it did not reach isolation, but narrowed down the candidates for new sulfur compounds of algae.

Academic Significance and Societal Importance of the Research Achievements

当研究成果で得られる新規知見により、重要性が高まる「硫黄生物学」の切り口から、「代謝機能」や「酸化還元システム」に対して、より拡張的で包括的な形の学術的理解の刷新を図れる。同時に、国民社会・経済産業的に有益な「新規の抗酸化機能性分子素材」としての潜在価値の評価が可能となる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization2021

    • Author(s)
      Kawano Yusuke、Suzuki Kengo、Ohtsu Iwao
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 85 Issue: 9 Pages: 1932-1936

    • DOI

      10.1093/bbb/zbab129

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defect of RNA pyrophosphohydrolase RppH enhances fermentative production of L-cysteine in <i>Escherichia coli</i>2020

    • Author(s)
      Morigasaki Susumu、Umeyama Akinori、Kawano Yusuke、Aizawa Yasushi、Ohtsu Iwao
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 6 Pages: 307-314

    • DOI

      10.2323/jgam.2019.12.004

    • NAID

      130007991933

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of high-throughput quantitative analytical method for l-cysteine-containing dipeptides by LC?MS/MS toward its fermentative production2019

    • Author(s)
      Kawano Yusuke、Shiroyama Maeka、Kanazawa Koji、Suzuki Yasushi A.、Ohtsu Iwao
    • Journal Title

      AMB Express

      Volume: 9(1) Issue: 1 Pages: 91-91

    • DOI

      10.1186/s13568-019-0817-2

    • NAID

      120007127832

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] バイオテクノロジー分野における硫黄化合物の網羅的解析技術がもたらす可能性2019

    • Author(s)
      新多智明、阿閉耕平、鈴木健吾、河野祐介、大津厳生
    • Journal Title

      日本食品工学会誌

      Volume: 20(2) Pages: 4-7

    • Related Report
      2019 Research-status Report
  • [Presentation] コーヒーの香り成分2-フランメタンチオール測定系の開発2022

    • Author(s)
      河野祐介、大津厳生
    • Organizer
      2021年度 遺伝研研究会「微生物バイオプロダクションの深化と展望」
    • Related Report
      2021 Annual Research Report
  • [Presentation] 様々な硫黄源が植物の生育と硫黄代謝に及ぼす影響2021

    • Author(s)
      丸山明子、河野祐介、大津厳生、中嶋孝嗣、大津直子、川口諒太、塩塚直輝、山口千仁、Khamsalath Soudthedlath、一瀬智美
    • Organizer
      日本土壌肥料学会2020年度岡山大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 硫黄代謝物の減少が宇宙滞在における肝障害に寄与している可能性2021

    • Author(s)
      黒澤亮、杉本良太、今井裕恵、阿閉耕平、山田康嗣、河野祐介、大津厳生、鈴木健吾
    • Organizer
      第10回 超異分野学会 本大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Metabolomic analysis of biological sulfur compounds using LC-MS/MS system and its potential2020

    • Author(s)
      Yusuke Kawano、Kengo Suzuki、Iwao Ohtsu
    • Organizer
      Hyper Interdisciplinary Conference in Malaysia 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 微生物の働きや酸化度の新たな評価方法: サルファーインデックス2019

    • Author(s)
      大津厳生、河野祐介、鈴木健吾
    • Organizer
      日本薬学会第139年会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 微生物が産生する特異な硫黄代謝物に基づくサルファーインダストリーの創出2019

    • Author(s)
      河野祐介、鈴木健吾、大津厳生
    • Organizer
      第13回メタボロームシンポジウム
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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