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Elucidation of the function and biosynthetic mechanism of the cell wall polysaccharide Nigeran of Aspergillus luchuensis

Research Project

Project/Area Number 19K15737
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Uechi Keiko  琉球大学, 農学部, 助教 (70733426)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsニゲラン / α-1,3-グルカン / 真菌細胞壁 / Aspergillus属 / 窒素源飢餓 / Aspergillus luchuensis / 細胞壁多糖 / 窒素飢餓 / 細胞壁 / Aspergillus
Outline of Research at the Start

糸状菌 Aspergillus属の一部は,窒素飢餓条件下でニゲランという細胞壁多糖を活発に生産する。しかし,その役割や生合成メカニズムについての知見はほとんどなかった。本研究では,申請者が実施した窒素飢餓条における糸状菌の遺伝子発現解析から見出したニゲラン合成酵素の機能解析や当該酵素遺伝子欠損株の細胞壁多糖の組成について検討し,糸状菌におけるニゲラン合成の意義やメカニズムの解明を目指す。

Outline of Final Research Achievements

The fungal cell wall is composed mainly of polysaccharides. Under nitrogen-free conditions, some Aspergillus spp. produce significant levels of nigeran, a fungal cell wall polysaccharide composed of alternating a-1,3/1,4-glucosidic linkages. The mechanisms regulating the biosynthesis and function of nigeran are unknown. Here, we performed RNA sequencing of Aspergillus luchuensis cultured under nitrogen-free or low-nitrogen conditions. A putative a-1,3-glucan synthase gene, whose transcriptional level was upregulated under nitrogen-free conditions, was demonstrated to encode nigeran synthase. Furthermore, two genes encoding an a-glucanotransferase and a hypothetical protein were shown to be involved in controlling the nigeran content and molecular weight.

Academic Significance and Societal Importance of the Research Achievements

本研究では,糸状菌のニゲラン合成酵素遺伝子をはじめて同定した。本酵素はα-1,3-グルカン合成酵素との相同性が高く,酵素化学的な多糖合成メカニズムはほぼ同様だと推定される。α-1,3-グルカンは真菌が宿主への病原性を発揮するための重要な構成成分であることから医学的な関心も高い。ニゲランはα-1,3-グルカンよりも容易に抽出できることから,ニゲラン合成研究で得た知見は,α-1,3-グルカン合成についても応用できることが期待される。また,ニゲランは新規のバイオマスベースプラスチック素材としての利用も期待できる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (7 results)

  • [Journal Article] Preparation and Crystal Structure Analysis of High-Strength Film Derived from Nigeran Ester Derivatives2022

    • Author(s)
      Togo Azusa、Uechi Keiko、Mizutani Osamu、Kimura Satoshi、Iwata Tadahisa
    • Journal Title

      Journal of Fiber Science and Technology

      Volume: 78 Issue: 8 Pages: 126-132

    • DOI

      10.2115/fiberst.2022-0017

    • ISSN
      2189-7654
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural Analysis and Construction of a Thermostable Antifungal Chitinase2022

    • Author(s)
      Kozome Dan、Uechi Keiko、Taira Toki、Fukada Harumi、Kubota Tomomi、Ishikawa Kazuhiko
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 88 Issue: 12

    • DOI

      10.1128/aem.00652-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in Aspergillus luchuensis2021

    • Author(s)
      Uechi Keiko、Yaguchi Hajime、Tokashiki Jikian、Taira Toki、Mizutani Osamu
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 87 Issue: 21

    • DOI

      10.1128/aem.01144-21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis and characterization of α-1,3-alt-α-1,4-glucan (nigeran) ester derivatives2021

    • Author(s)
      Togo Azusa、Uechi Keiko、Mizutani Osamu、Kimura Satoshi、Iwata Tadahisa
    • Journal Title

      Polymer

      Volume: 214 Pages: 123343-123343

    • DOI

      10.1016/j.polymer.2020.123343

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 黒麹菌細胞壁構成多糖ニゲランの高生産化に関する研究2022

    • Author(s)
      水谷治,與古田佳世,阿部多恵,上地敬子,外山博英
    • Organizer
      第74回大会日本生物工学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 黒麹菌細胞壁多糖ニゲランの合成酵素遺伝子発現に関わる転写因子の探索2022

    • Author(s)
      上地敬子,平良東紀,水谷治
    • Organizer
      第74回大会日本生物工学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 黄麹菌ΔAG-GAG株におけるニゲラン高生産株の造成2022

    • Author(s)
      阿部多恵,與古田佳世,阿部啓悦,外山博英,上地敬子,水谷治
    • Organizer
      第21回糸状菌分子生物学コンファレンス
    • Related Report
      2022 Annual Research Report
  • [Presentation] Paenibacillus sp. A13由来マイコデキストラナーゼの酵素化学的諸性質の比較と触媒残基の特定2021

    • Author(s)
      平田風子、山内夢乃、稲福隆之、上地敬子、平良東紀
    • Organizer
      日本生物工学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 黒麹菌 Aspergillus luchuensis のニゲラン合成酵素遺伝子群の同定と機能解析2021

    • Author(s)
      上地敬子、渡嘉敷直杏、平良東紀、水谷治
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Paenibacillus sp. A13由来マイコデキストラナーゼの酵素化学的諸性質の比較2020

    • Author(s)
      平田風子、山内夢乃、稲福隆之、上地敬子、平良東紀
    • Organizer
      日本農芸化学会西日本支部大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 黒麹菌 Aspergillus luchuensis が生産する 細胞壁多糖ニゲランの合成関連遺伝子の解析2019

    • Author(s)
      上地敬子
    • Organizer
      糸状菌コンファレンス
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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