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Identification of the dwarf male substances in a moss

Research Project

Project/Area Number 19K15759
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38040:Bioorganic chemistry-related
Research InstitutionTokyo University of Agriculture and Technology (2021)
Keio University (2019-2020)

Principal Investigator

MIYAZAKI Sho  東京農工大学, 学内共同利用施設等, 助教 (30755955)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords矮化 / 天然物 / コケ / 天然物化学 / 植物科学 / 生合成 / 次世代シークエンサ
Outline of Research at the Start

コケ植物が生産する【矮雄誘導物質】の単離・構造決定と生合成経路の解明のため,生物検定法を用いた【矮雄誘導物質】の検出が最重要課題と位置付けている.続いて【矮雄誘導物質】の構造情報を質量分析装置やNMR等で取得するとともに,その物質が活性本体かどうかを証明したい.並行して次世代シークエンサを用いたトランスクリプトーム解析を実施し,生合成経路の解明に取り組みたいと考えている.

Outline of Final Research Achievements

A kind of male strains of moss plants perceived a signal from the female strain to reduce the individual size, and grow in a semi-parasitic state on the female strain. I thought that it was most important to construct a reproduction experimental system that challenged the search for the substance controls this phenomenon, and mainly examined the systems that had been cultivated so far, but could not find a stable experimental system. However, I found that one or more plant hormones could be involved in this phenomenon from non-sterile culture system.

Academic Significance and Societal Importance of the Research Achievements

本研究で着目した現象と物質は植物の新たな繁殖戦略を物質レベルで明らかにできるものであり,重要な知見を提供できるものと期待する。植物には一つの花に雌しべと雄しべが共存する両性花,一つの株に雄花と雌花をつける雌雄異花,そして動物のようにメスとオスが別れている雌雄異株の形態が存在する。受精の効率,環境への適応性を考えると,どの形態も一長一短である。このような中,雌雄異株の形態をとったにも関わらず,受精の効率をあげるため矮雄という戦略をとったコケ植物の制御機構を明らかにできれば,植物の繁殖戦略と性分化の新たな概念を提唱できると期待できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Histological and quantitative image analyses of rice gall formed by orange leafhopper Cicadulina bipunctata (Hemiptera: Cicadellidae)2021

    • Author(s)
      Miyazaki Sho、Endo Aoi、Matsui Soh、Tokuda Makoto、Saikawa Yoko
    • Journal Title

      Applied Entomology and Zoology

      Volume: 56 Issue: 3 Pages: 419-425

    • DOI

      10.1007/s13355-021-00736-8

    • NAID

      40022626814

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
  • [Journal Article] Genomic evidence for convergent evolution of gene clusters for momilactone biosynthesis in land plants2020

    • Author(s)
      Mao L, Kawaide H, Higuchi T, Chen M, Miyamoto K, Hirata Y, Kimura H, Miyazaki S, Teruya M, Fujiwara K, Tomita K, Yamane H, Hayashi K, Nojiri H, Jia L, Qiu J, Ye C, Timko MP, Fan L, and Okada K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 117 Issue: 22 Pages: 12472

    • DOI

      10.1073/pnas.1914373117

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Orange Leafhopper Cicadulina bipunctata Feeding Induces Gall Formation Nitrogen Dependently and Regulates Gibberellin Signaling2020

    • Author(s)
      Miyazaki Sho、Kasahara Keita、Matsui Soh、Tokuda Makoto、Saikawa Yoko
    • Journal Title

      Plants

      Volume: 9 Issue: 10 Pages: 1270-1270

    • DOI

      10.3390/plants9101270

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The apple gene responsible for columnar tree shape reduces the abundance of biologically active gibberellin2020

    • Author(s)
      Watanabe Daichi、Takahashi Ikuo、Jaroensanti‐Tanaka Naiyanate、Miyazaki Sho、Jiang Kai、Nakayasu Masaru、Wada Masato、Asami Tadao、Mizutani Masaharu、Okada Kazuma、Nakajima Masatoshi
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 4 Pages: 1026-1034

    • DOI

      10.1111/tpj.15084

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a cyanidin-3-O-β-galactoside in gall tissue induced by midges on Japanese beech (Fagus crenata Blume)2019

    • Author(s)
      Miyazaki Sho、Kurisu Hironosuke、Nakata Masaya、Saikawa Yoko
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 84 Issue: 4 Pages: 797-799

    • DOI

      10.1080/09168451.2019.1698941

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli2019

    • Author(s)
      Miyazaki Sho、Nakajima Masatoshi、Kawaide Hiroshi
    • Journal Title

      Methods Mol Biol

      Volume: 1924 Pages: 35-43

    • DOI

      10.1007/978-1-4939-9015-3_4

    • ISBN
      9781493990146, 9781493990153
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] ワラビー萎縮症誘発物質の同定に向けた生物試験法の検討2021

    • Author(s)
      宮崎 翔,遠藤 碧,松井 奏,笠原 慶太,徳田 誠,犀川 陽子
    • Organizer
      日本農薬学会第46回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ワラビー萎縮症誘発物質の同定に向けたイネ幼苗を用いた試験法の検討2020

    • Author(s)
      宮崎 翔,笠原 慶太,徳田 誠,犀川 陽子
    • Organizer
      日本農薬学会第45回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 虫えい形成における寄主植物の形態変化を誘導する物質の探索研究2019

    • Author(s)
      宮崎 翔, 栗栖泰之介, 笠原慶太, 徳田 誠, 中田雅也, 犀川陽子
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 虫えい内生菌の生産する植物成長制御物質の探索2019

    • Author(s)
      松井 奏, 並木航太, 惠枝, 高岡春乃, 後藤加奈, 栗栖泰之介, 宮崎 翔, 中田雅也, 犀川陽子
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Identification of ancestral gibberellin in Physcomitrella patens2019

    • Author(s)
      Sho Miyazaki
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2019-04-18   Modified: 2023-01-30  

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