| Project/Area Number |
19K15763
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
Tan Hui 北海道大学, 保健科学研究院, 客員研究員 (10821671)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Sea Mussel / Oxidative stress / Keap-Nrf2-ARE pathway / Keap1-Nrf2-ARE pathway / Oxidative Stress / Functional food / Hokkaido / Nrf2 / Functional foods |
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| Outline of Research at the Start |
Hokkaido contains a large area of Japan, and its weather and geographical conditions vary between regions, which offer us diverse possibilities for screening novel Nrf2 activators and developing anti-oxidant functional foods.
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| Outline of Final Research Achievements |
Epidemiological studies have demonstrated the dietary antioxidants can help reduce the effects of oxidative stress, thereby exerting immune regulation. Activation of Nrf2 is a crucial cellular defence mechanism against oxidative stress. We found ethanol extract of sea mussel provided significant protection against AAPH-induced cytotoxicity in HepG2 cells. Also, we found that EtOH extract of sea mussel showed dose dependent effect on ARE activation. The mechanism underlying its cytoprotective effect caused by significantly indued the multitude of Nrf2 genes, including OH-1, NQO1, GCLC, GCLM, GPx, GR and SOD1. Further studies reveal that sea mussel EtOH extract induced the expression of NQO1 and GSH activity. We further isolated the active fractions from ethonal extract by bioactivity-guided fractionation assay. As a result, Fr. 5 showed strongest activity and Fr 6 showed moderate activity on activation Keap1-Nrf2-ARE pathway.
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| Academic Significance and Societal Importance of the Research Achievements |
We investigated the protective effect of sea mussel extract on AAPH-induced oxidative stress via activation of Keap-Nrf2-ARE signalling and our finding suggests that sea mussel extract and its subfractions could be a valuable food-derived material capable of improving liver related diseases.
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