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Multi-omics authentication of global and adulterated Japanese non-centrifugal brown sugars

Research Project

Project/Area Number 19K15784
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38050:Food sciences-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

ASIKIN YONATHAN  琉球大学, 農学部, 准教授 (20799689)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsFood flavor quality / Mineral composition / MS-e-nose profiling / Multi-omics profiling / Non-centrifugal sugar / Brown sugar / Product adulteration / Food authentication
Outline of Research at the Start

Non-centrifugal brown sugar is widely produced in many countries under different forms and flavor characteristics. Hence, the global trade era has urge domestic sugar industries to take measure on fraud and adulteration threats. This study aims to elucidate nutrient and flavor markers of brown sugars across the globe through multi-omics analytical technique, and thus to advance the building efforts of international product identity for brown sugar against adulteration. Moreover, authentication of brown sugar flavor can be unraveled through improved MS-based-e-nose omics profiling technology.

Outline of Final Research Achievements

Unrefined-solidified sugars which are derived from various plant sources, including sugarcane, palm, and coconut, had diverse key quality traits. Non-centrifugal cane sugar (NCS) had higher polyphenol contents and antioxidant activities than in palm- and coconut-sugars. Traditionally made NCS contained greater Maillard reaction products (MRPs) and less acetic acid than in industrially manufactured NCS. Production origin also greatly affected mineral and volatile organic compound (VOC) compositions of NCS. MS-e-nose analysis showed comparable VOC profiles between NCSs of Japan and ASEAN origins. Moreover, Okinawan specialty NCS (kokuto) can be adulterated with raw sugar, forming a processed NCS (kako-kokuto). The color of adulterated NCS became lighter as raw sugar proportion increased, and it contained less VOCs, MRPs, and organic acids. The adulteration also lowered sourness and roasted aroma intensities, and thus distinctly altered its flavor quality and retronasal olfaction traits.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、原料材料や原料産地の異なる黒糖における栄養学的、食品機能性、香気特性等の特徴を示す重要な情報を提供するものである。これらデータは、食品・飲料の製造において黒糖を原材料とする場合、官能特性や嗜好性を着目した商品開発や改良するための基礎的知見として活用できる。さらに、この研究では、黒糖への粗糖混合比率を判別するためのマルチオミックス分析手法の開発も行っており、これら手法を用いることで、多角的に沖縄県産黒糖の品質価値を国際的に示すことにもつながると考えている。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2023 2022 2021 2019 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Int'l Joint Research] Naresuan University(タイ)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Composition of Minerals and Volatile Organic Components of Non-Centrifugal Cane Sugars from Japan and ASEAN Countries2023

    • Author(s)
      Ayustaningwarno Fitriyono、Asikin Yonathan、Amano Ryo、Vu Nam Tuan、Hajar-Azhari Siti、Anjani Gemala、Takara Kensaku、Wada Koji
    • Journal Title

      Foods

      Volume: 12 Issue: 7 Pages: 1406-1406

    • DOI

      10.3390/foods12071406

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physicochemical, antioxidant, volatile component, and mass spectrometry-based electronic nose analyses differentiated unrefined non-centrifugal cane, palm, and coconut sugars2021

    • Author(s)
      Monthana Weerawatanakorn, Yonathan Asikin, Kanyaphat Meerod, Hajime Tamaki, Koji Wada
    • Journal Title

      Journal of Food Measurement and Characterization

      Volume: 15 (2) Issue: 2 Pages: 1563-1577

    • DOI

      10.1007/s11694-020-00749-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Flavor components and retronasal olfaction profiles of simulated adulterated brown sugar2023

    • Author(s)
      Yonathan Asikin, Yuki Nakaza, Eito Tsuchida, Hirotaka Kaneda, Kensaku Takara, Koji Wada
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 黒糖の香気特性とレトロネーザルアロマプロファイルの解析2022

    • Author(s)
      仲座佑紀, 前田剛希, 土田永渡, 金田弘拳, 新垣江利子, 和田浩二, Yonathan Asikin
    • Organizer
      日本食品保蔵科学会第71回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Composition of volatile aroma compounds and minerals of non-centrifugal sugars from Japan and ASEAN countries2022

    • Author(s)
      Yonathan Asikin, Ryo Amano, Eito Tsuchida, Kensaku Takara, Koji Takabatake, Koji Wada
    • Organizer
      日本食品科学工学会第69回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] GC-MS based electronic nose profiling of regional brown sugars2021

    • Author(s)
      Yonathan Asikin
    • Organizer
      The 6th International Conference on Food, Agriculture, and Natural Resources 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Improvement of non-centrifugal brown sugar quality through food science study2021

    • Author(s)
      Yonathan Asikin
    • Organizer
      The 1st International Conference on Applied Science and Technology for Community Service
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 黒糖製造工程の仕上げ加熱の違いによる物理化学的特性および香気成分の分析2021

    • Author(s)
      仲座佑紀, Yonathan Asikin, 大江萌那, 高良健作, 前田剛希, 土田永渡, 和田浩二
    • Organizer
      日本食品保蔵科学会第70回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Sugar, organic acid, and MS-e-nose properties of non-centrifugal cane sugars from Japan and ASEAN countries2021

    • Author(s)
      Asikin Yonathan, Wada Koji, Tsuchida Eito, Maeda Goki, Nam Vu Tuan, Ayustaningwarno Fitriyono, Hajar-Azhari Siti
    • Organizer
      The 2021 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Physico-chemical quality discrimination and volatile flavor profiling of regional non-centrifugal sugar made from sugarcane, palm, and coconut2019

    • Author(s)
      Yonathan Asikin, Miyako Kusano, Monthana Weerawatanakorn, Kanyaphat Meerod, Hajime Tamaki, Koji Wada
    • Organizer
      13th Asian Congress of Nutrition, Bali, Indonesia, アジア栄養学会連合 (Federation of Asian Nutrition Societies: FANS) (Ann Nutr Metab 2019;75:1-424: DOI: 10.1159/000501751)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2024-01-30  

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