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Analysis of sulfur-dependent energy metabolism in yeast

Research Project

Project/Area Number 19K15803
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Nishimura Akira  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (30781728)

Project Period (FY) 2019-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords活性イオウ / エネルギー代謝 / イオウ呼吸 / 酵母 / 発酵
Outline of Research at the Start

発酵生産は生育に必要なエネルギーを目的の有用物質の代謝に活用するため、目的物質の生産性が増加するに伴い、生育遅延がしばしば起こる。従って、目的物質の生産性の向上は、細胞の生育と生産性のバランスを上手く維持しながら、これまで進められてきた。しかし、細胞が有するエネルギーの総量が決まっているため、従来の代謝工学を活用した方法には限界がきている。最近、応募者らは、酵母を含む真核生物において、新規なエネルギー代謝である「イオウ呼吸」を発見した。本研究では、酵母におけるイオウ呼吸を巧みに利用することで、生育と生産性を共に増強させるエネルギー代謝機構に基づき、発酵生産を飛躍的に改良する手段を提唱する。

Outline of Final Research Achievements

we attempted to understand physiological functions of reactive sulfur species (RSS) using yeast Saccharomyces cerevisiae, a useful model for eukaryotic biology. First, we found that yeast has a pyridoxal phosphate (PLP)-dependent biosynthesis of RSS by cysteinyl-tRNA synthetase (CARS), similar to bacteria and mammal. Next, our study indicated that a yeast mutant of PLP-binding site, which possessed the intact protein synthesis, dramatically reduces chronological aging, compared with wild-type, suggesting that CARS-produced RSS may play an important role in regulating longevity and sustaining the life span.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、発酵科学へ応用のみならず、エネルギー代謝異常が関わる各種疾患(加齢、ミトコンドリア病、生活習慣病、がん等)の発症機構の理解と予防・治療法の開発に大きく寄与することが期待される。また、活性イオウ分子の産生系をターゲットにした抗真菌薬の開発も抗菌・真菌薬開発も期待される。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2021 2020 2019 Other

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Downregulation of the broad-specificity amino acid permease Agp1 mediated by the ubiquitin ligase Rsp5 and the arrestin-like protein Bul1 in yeast2021

    • Author(s)
      Tanahashi Ryoya、Matsushita Tomonori、Nishimura Akira、Takagi Hiroshi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: - Issue: 5 Pages: 1266-1274

    • DOI

      10.1093/bbb/zbab028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of arginine on proline utilization in Saccharomyces cerevisiae2020

    • Author(s)
      Akira Nishimura, Tsubasa Tanikawa, Hiroshi Takagi
    • Journal Title

      Yeast

      Volume: 37 Issue: 9-10 Pages: 531-540

    • DOI

      10.1002/yea.3504

    • NAID

      120007146507

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The yeast α-arrestin Art3 is a key regulator for arginine-induced endocytosis of the high-affinity proline transporter Put42020

    • Author(s)
      Akira Nishimura, Ryoya Tanahashi, Hiroshi Takagi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 531 Issue: 3 Pages: 416-421

    • DOI

      10.1016/j.bbrc.2020.07.117

    • NAID

      120007147755

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of a novel variant of the yeast γ-glutamyl kinase Pro1 on its enzymatic activity and sake brewing2020

    • Author(s)
      Murakami Naoyuki、Kotaka Atsushi、Isogai Shota、Ashida Keiko、Nishimura Akira、Matsumura Kengo、Hata Yoji、Ishida Hiroki、Takagi Hiroshi
    • Journal Title

      Journal of Industrial Microbiology and Biotechnology

      Volume: 47 Issue: 9-10 Pages: 715-723

    • DOI

      10.1007/s10295-020-02297-1

    • NAID

      120007147752

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The C2 domain of the ubiquitin ligase Rsp5 is required for ubiquitination of the endocytic protein Rvs167 upon change of nitrogen source2020

    • Author(s)
      Tanahashi Ryoya、Afiah Tira Siti Nur、Nishimura Akira、Watanabe Daisuke、Takagi Hiroshi
    • Journal Title

      FEMS Yeast Research

      Volume: 20 Issue: 7

    • DOI

      10.1093/femsyr/foaa058

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of Protein Function Through Oxidation and Reduction of Persulfidated States2020

    • Author(s)
      E Doka, T Ida, M Dagnell, Y Abiko, N C Luong, N Balog, T Takata, B Espinosa, A Nishimura, Q Cheng, Y Funato, H Miki, J M Fukuto, J R Prigge, E E Schmidt, E S J Arner, Y Kumagai, T Akaike, P Nagy
    • Journal Title

      Sci Adv

      Volume: 6 Issue: 1 Pages: 8358-8358

    • DOI

      10.1126/sciadv.aax8358

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells2019

    • Author(s)
      Nishimura Akira、Nasuno Ryo、Yoshikawa Yuki、Jung Minkyung、Ida Tomoaki、Matsunaga Tetsuro、Morita Masanobu、Takagi Hiroshi、Motohashi Hozumi、Akaike Takaaki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 294 Issue: 37 Pages: 13781-13788

    • DOI

      10.1074/jbc.ra119.009203

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アルギニンによる酵母のプロリン資化抑制機構2021

    • Author(s)
      西村 明、高木 博史
    • Organizer
      日本農芸化学学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 酵母Saccharomyces cerevisiaeにおけるプロリン資化抑制機構の理解2020

    • Author(s)
      西村 明、棚橋 亮弥、高木 博史
    • Organizer
      日本生化学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 酵母Saccharomyces cerevisiaeにおけるアルギニンによるプロリン資化抑制機構の解析2020

    • Author(s)
      西村 明、谷川 翼、棚橋 亮弥、高木 博史
    • Organizer
      日本醸造学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Biosynthesis and physiological functions of reactive cysteine persulfides2019

    • Author(s)
      Akira Nishimura, Tomoaki Ida, Masanobu Morita, Tetsuro Matsunaga, Hiroshi Takagi, Hozumi Motohashi, Takaaki Akaike
    • Organizer
      SFRR Asia
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 活性パースルフィドの種横断的な生理機能の解明2019

    • Author(s)
      西村 明、井田 智章、守田 匡伸、松永 哲郎、高木 博史、本橋 ほづみ、赤池 孝章
    • Organizer
      日本生化学会
    • Related Report
      2019 Research-status Report
  • [Remarks] リサーチマップ(西村明)

    • URL

      https://researchmap.jp/akira_nishimura

    • Related Report
      2020 Annual Research Report
  • [Remarks] NAIST 高木研のホームページ

    • URL

      https://bsw3.naist.jp/takagi/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2022-01-27  

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