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Characterizaion of interspecific hybridization barrier of fruit trees in the Rosaceae and its application towards depeloping new hybrid crops

Research Project

Project/Area Number 19K15834
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionKyoto Prefectural University

Principal Investigator

MORIMOTO TAKUYA  京都府立大学, 生命環境科学研究科, 講師 (90837634)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords遠縁交雑 / 属間雑種 / 種間障壁 / リンゴ亜連 / バラ科果樹 / ゲノム解析
Outline of Research at the Start

植物には,異なる種との交雑を意図的に避ける生殖機構が存在しており,異種間の交雑は通常成立しない.一方で,稀にこの種間障壁が打破されると新しい特性を持った新種が誕生する.申請者はバラ科リンゴ亜連に属する果樹は非常に多様な種間障壁パターンを示し,交雑障壁機構の全貌解明に向けた重要なモデルとなることを見出した.本研究の目的は,リンゴ亜連果樹の交雑障壁機構を解明し,それを打破する技術開発を行うことである.本研究成果は異種ゲノム融合技術の開発に直結するものであり,異種間での自由な遺伝子のやり取りを可能にするという点で,従来の育種法の概念を覆す技術革新となる.

Outline of Final Research Achievements

Characterization of underling mechanism on interspecific hybridization barrier would be a fundamental step towards the development of new hybrid crops. In this study, we investigated genetic bases of intergeneric hybridization barrier between apple (Malus) and pear (Pyrus), two important fruit trees in the family Rosaceae. Application of genome-wide analysis enabled us to identify a single genomic locus on the middle part of chromosome 5 that control the intergeneric barrier. Comparative genome analysis targeting on this region identified a large insertion sequence specific to Japanese pear (Pyrus pyrifolia) genome. Among genes located on the identified genomic region, we detected promising candidate genes, which shows species-specific expression in pollen as well as allele-specific expression.

Academic Significance and Societal Importance of the Research Achievements

近年の気候変動によって作物を取り巻く生育環境は刻々と変化しており,新しい生産体系の構築に向けて,遺伝的多様性を増加させることは育種プログラムの最重要課題である.有効な手法の一つとなるのが種間交雑であるが,多くの場合,種間障壁によって雑種形成が阻害される.本研究では,主要果樹であるリンゴとナシの属間交雑の成否を制御するゲノム領域を同定し,果樹の新規雑種の作出にむけた基盤を提供した.果樹は近縁種間で多様な種間障壁を形成しており,その機構解明は農学的な意義に加えて,種の分化・形成といった進化学のモデルにもなりうることを示した.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2021 2019 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Characterization of transcriptomic response in ovules derived from inter-subgeneric hybridization in Prunus (Rosaceae) species2021

    • Author(s)
      Morimoto Takuya、Kitamura Yuto、Numaguchi Koji、Itai Akihiro
    • Journal Title

      Plant Reproduction

      Volume: 34 Issue: 3 Pages: 255-266

    • DOI

      10.1007/s00497-021-00423-2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of post-mating interspecific cross-compatibility in Prunus (Rosaceae)2019

    • Author(s)
      Morimoto Takuya、Kitamura Yuto、Numaguchi Koji、Akagi Takashi、Tao Ryutaro
    • Journal Title

      Scientia Horticulturae

      Volume: 246 Pages: 693-699

    • DOI

      10.1016/j.scienta.2018.11.045

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic mapping of a locus controlling the intergeneric hybridization barrier between apple and pear2019

    • Author(s)
      Morimoto Takuya、Inaoka Maia、Banno Kiyoshi、Itai Akihiro
    • Journal Title

      Tree Genetics & Genomes

      Volume: 16 Issue: 1 Pages: 1-11

    • DOI

      10.1007/s11295-019-1397-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Genetic mapping of a locus controlling the intergeneric hybridization barrier between apple and pear2021

    • Author(s)
      Takuya Morimoto, Akihiro Itai
    • Organizer
      American Society for Horticulture Science Annual Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リンゴとナシの属間交雑を制御するゲノム領域の解析2021

    • Author(s)
      関口遼,森本拓也,板井章浩
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Characterization of intergeneric cross-compatibility between apple and pear2021

    • Author(s)
      Ryo Sekiguchi, Takuya Morimoto, Akihiro Itai
    • Organizer
      3rd DLSAU International Multidisciplinary Research Conference
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] バラ科リンゴ亜連果樹における異種間交雑親和性2021

    • Author(s)
      松田悠之介,森本拓也,関口遼,板井章浩
    • Organizer
      園芸学会
    • Related Report
      2020 Research-status Report
  • [Presentation] リンゴ属とナシ属の交雑(不)和合性を支配するゲノム領域の同定2019

    • Author(s)
      森本拓也、稲岡麻衣亜、伴野潔、板井章浩
    • Organizer
      園芸学会秋季大会
    • Related Report
      2019 Research-status Report
  • [Remarks] 京都府立大学 果樹園芸学研究室【森本研究室】

    • URL

      https://eureka.kpu.ac.jp/~morimoto/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 京都府立大学 果樹園芸学研究室【森本研究室】

    • URL

      https://eureka.kpu.ac.jp/~morimoto/publications.html

    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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