| Project/Area Number |
19K15846
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
GAN PAMELA 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (80634740)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Project Status |
Discontinued (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | Genome rearrangement / cluster / virulence / fungal genome / anthracnose / nudix / effector / Keywords / fungi / genome / evolution / Colletotrichum / pathogen / Pathogen / Evolution / Fungi / Effector |
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| Outline of Research at the Start |
Understanding how virulence evolves in pathogens can contribute to the development of better methods of disease control. We discovered a virulent strain-specific gene cluster in Colletotrichum fungi that has a variable distribution across multiple species. We will generate chromosome level assemblies for different species with this virulence cluster to gain insights into the evolution and transmission of these genes. We will also functionally characterize a gene within the cluster, which has been shown in preliminary experiments to suppress basal plant defense responses.
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| Outline of Annual Research Achievements |
Understanding how virulence emerges in pathogens can contribute to the development of better methods of disease control. We discovered a virulent strain-specific cluster of genes in Colletotrichum fungi which cause strawberry anthracnose that has an uneven phylogenetic distribution across multiple species. In FY2020, the following was achieved:
1. Submitted paper describing potential virulence gene clusters in sub-telomeric regions and repeat-rich chromosomes. The work and associated genome and transcriptome data has been publicly released on NCBI, github and Biorxiv and is currently under review in the journal Genome Research.
2. One of the proteins present in the potential virulence gene cluster, CfNudix, was characterized further. Phylogenetic analyses of all C. fructicola nudixes including CfNudix was carried out to determine the most closely related sequences to identify potential substrates by homology to previously characterized nudixes. The substrate specificity of purified CfNudix was tested on known nudix substrates.
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