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Investigating the mechanisms of attraction, settlement, and boring to the host by pest polychaetes in shellfish aquaculture and their origins

Research Project

Project/Area Number 19K15899
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionIshinomaki Senshu University (2022)
Iwate Medical University (2019-2021)

Principal Investigator

Abe Hirokazu  石巻専修大学, 理工学部, 准教授 (10784192)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords海産環形動物 / 貝類養殖 / スピオ科 / 穿孔性多毛類 / 分子系統 / 発現遺伝子 / ミトゲノム / 18S rRNA / 28S rRNA / 16S rRNA / 遺伝子配置 / 嗅覚受容体 / 酸分泌 / RNA-seq
Outline of Research at the Start

貝類養殖の害虫である穿孔性多毛類は,幼生が宿主となる貝類に選択的に着底し,貝殻に穿孔して内部に侵入するという興味深い共生生態を示すため,共生生活の確立(宿主への幼生の誘引)や宿主への生理的適応(貝殻への穿孔)という観点から進化生物学的に興味深い研究材料である。本研究では,網羅的発現遺伝子解析により,宿主からの誘引物質を認識するための幼生の嗅覚受容体の特定と,穿孔(酸分泌)の分子メカニズムの解明を行う。同時に,分子系統解析により穿孔性の獲得に至る系統進化のプロセスを推定することで,誘引物質に対する嗅覚受容体や酸分泌メカニズムの起源とその進化プロセスを探る。

Outline of Final Research Achievements

This study aims to clarify the mechanisms of "larval attraction and settlement to the host" and " boring" in pest spionid polychaetes in shellfish aquaculture, and to understand the "evolutionary process" in the development of such mechanisms. In this study, the following investigations were conducted: 1) estimation of phylogenetic relationships among 15 genera and 62 species of free-living and symbiotic/boring spionid polychaetes based on molecular phylogenetic analysis; 2) description of two new species of spionid polychaetes showing a novel symbiotic system, and the description and estimation of phylogenetic position of one new species of the enigmatic genus Atherospio; 3) description of the morphology of the planktonic larvae of 41 species in 14 genera of the family Spionidae; 4) interspecific comparison of gene expression profiles in adults and larvae of four free-living and boring species belonging to three genera in the family Spionidae.

Academic Significance and Societal Importance of the Research Achievements

厳密に宿主を認識して着底する共生性の海産無脊椎動物では,浮遊幼生の嗅覚受容体が宿主認識に重要な役割を果たしている可能性がある。本研究では,「幼生期の誘引物質に対する嗅覚受容体の変化が着底基質選択性や宿主の転換を駆動する」という仮説のもと,スピオ科多毛類の生息基質や宿主の変化プロセスに合わせた嗅覚受容体の分子的変化を追うことで,基質選択性や宿主の転換が起こる仕組みを解明することを目指したものである。このアイデアは,海産無脊椎動物における着底基質選択や宿主転換を統一的に説明できるモデルとして広く波及効果が期待でき,海洋生物の種分化や多様化のメカニズムの理解につながる可能性を秘めているといえる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (28 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (9 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 3 results) Remarks (3 results)

  • [Int'l Joint Research] Normandie University(フランス)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Stellenbosch University(南アフリカ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Hofstra University/University of Maine/Dancing Coyote Environmental(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Universite de Caen(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Academia Sinica, Taiwan(その他の国・地域)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Stellenbosch University(南アフリカ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Hofstra University/University of Maine(米国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Universite de Caen(フランス)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Academia Sinica, Taiwan(その他の国・地域)

    • Related Report
      2019 Research-status Report
  • [Journal Article] A novel symbiotic relationship between ascidians and a new tunic-boring polychaete (Annelida: Spionidae: Polydora)2022

    • Author(s)
      ABE HIROKAZU、HOSHINO OSAMU、YAMADA KAZUYUKI、OGINO TETSUYA、KAWAIDA SHUN、SATO-OKOSHI WAKA
    • Journal Title

      Zootaxa

      Volume: 5159 Issue: 1 Pages: 1-22

    • DOI

      10.11646/zootaxa.5159.1.1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogenetic position of the enigmatic genus Atherospio and description of Atherospio aestuarii sp. nov. (Annelida: Spionidae) from Japan2022

    • Author(s)
      Abe Hirokazu、Kan Kotaro
    • Journal Title

      PeerJ

      Volume: 10 Pages: e13909-e13909

    • DOI

      10.7717/peerj.13909

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Polydorid species (Annelida: Spionidae) associated with commercially important oyster shells and their shell infestation along the coast of Normandy, in the English Channel, France2022

    • Author(s)
      Sato-Okoshi Waka、Okoshi Kenji、Abe Hirokazu、Dauvin Jean-Claude
    • Journal Title

      Aquaculture International

      Volume: 31 Issue: 1 Pages: 195-230

    • DOI

      10.1007/s10499-022-00971-y

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan2021

    • Author(s)
      Abe Hirokazu、Sato‐Okoshi Waka
    • Journal Title

      ZooKeys

      Volume: 1015 Pages: 1-86

    • DOI

      10.3897/zookeys.1015.54387

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel symbiotic relationship between a spionid polychaete and Lingula (Brachiopoda: Lingulata: Lingulidae), with description of Polydora lingulicola sp. nov. (Annelida: Spionidae)2020

    • Author(s)
      Abe H, Sato-Okoshi W
    • Journal Title

      Zoosymposia

      Volume: 19 Issue: 1 Pages: 103-120

    • DOI

      10.11646/zoosymposia.19.1.13

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clarifying the cryptogenic species Polydora neocaeca (Annelida: Spionidae): a shell-boring invasive pest of molluscs from locations worldwide2020

    • Author(s)
      Malan, A., Williams, J.D., Abe, H., Sato-Okoshi, W., Matthee, C.A., Simon, C.A.
    • Journal Title

      Marine Biodiversity

      Volume: In press

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 国内におけるAtherospio属未記載種(環形動物門:スピオ科)の報告とその系統的位置2022

    • Author(s)
      阿部博和・菅孔太朗
    • Organizer
      2022年日本プランクトン学会・日本ベントス学会合同大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環形動物の環:幼生研究から広がる分類・生態・進化学2021

    • Author(s)
      阿部博和
    • Organizer
      2021年日本ベントス学会・日本プランクトン学会 合同大会 日本ベントス学会
    • Related Report
      2021 Research-status Report
  • [Presentation] スピオ科多毛類(環形動物門)4種のミトコンドリアゲノム解析:系統的位置の推定と遺伝子配置の再配列2020

    • Author(s)
      阿部博和, 伊藤 萌, 中嶋信美, 小林元樹
    • Organizer
      日本動物学会 第91回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Polydorid species (Annelida, Spionidae) associated with calcareous substrates from Normandy, France2019

    • Author(s)
      Sato-Okoshi, W, Okoshi, K, Dauvin, J.-C, Abe, H
    • Organizer
      13th International Polychaete Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel symbiotic relationship between ascidians and an undescribed tunic-boring polychaete (Annelida: Spionidae: Polydora)2019

    • Author(s)
      Abe, H, Yamada, K, Hoshino, O, Ogino, T, Kawaida, S, Sato-Okoshi, W
    • Organizer
      13th International Polychaete Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多様化・進化研究の材料としてのスピオ科多毛類2019

    • Author(s)
      阿部博和
    • Organizer
      2019年日本ベントス学会・日本プランクトン学会合同大会 自由集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 日本産Spio属(環形動物門:スピオ科)の分類学的再検討2019

    • Author(s)
      阿部博和
    • Organizer
      2019年日本ベントス学会・日本プランクトン学会合同大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Identification guide to the planktonic larvae of marine annelids in Japan2019

    • Author(s)
      Abe, H, Kondoh, T, Sato-Okoshi, W
    • Organizer
      The Fourth Asian Marine Biology Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Survey of the family Spionidae (Annelida) in North Taiwan2019

    • Author(s)
      Sato-Okoshi, W, Abe, H, Kobayashi, G, Hsieh, H.-L, Chen, C.-P
    • Organizer
      The Fourth Asian Marine Biology Symposium
    • Related Report
      2019 Research-status Report
  • [Presentation] 石川県七尾湾海岸のベントス相2019

    • Author(s)
      海上智央・加藤雅文・青木美鈴・多留聖典・鈴木孝男・荒井美穂子・阿部博和・伊藤萌・金谷弦
    • Organizer
      2019年日本ベントス学会・日本プランクトン学会合同大会
    • Related Report
      2019 Research-status Report
  • [Remarks] 【研究】世界2例目となる被嚢穿孔性のゴカイ類を発見

    • URL

      https://www.senshu-u.ac.jp/ishinomaki/news/nid00016671.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 【新種記載】明らかに珍種!「ホヤノポリドラ」

    • URL

      https://isu-bi-blog.blogspot.com/2022/10/blog-post.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 【新種記載】度重なる奇遇。「イリエノギスピオ」

    • URL

      https://isu-bi-blog.blogspot.com/2022/11/blog-post_2.html

    • Related Report
      2022 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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