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Elucidation of a new regulatory mechanism for the regulation of female sexual behavior: Focusing on novel estrogen-responsive peptides

Research Project

Project/Area Number 19K15966
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42010:Animal production science-related
Research InstitutionNippon Veterinary and Life Science University (2020-2022)
Nippon Medical School (2019)

Principal Investigator

Watanabe Youki  日本獣医生命科学大学, 応用生命科学部, 助教 (50781788)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsロードシス / エストロジェン / 発情行動 / Npbwr1 / エストロゲン受容体 / 視床下部
Outline of Research at the Start

家畜受胎率の低下要因の一つに、微弱発情などの発情行動異常が挙げられる。発情行動発現の脳内機構の詳細は未だ不明な点が多い。本研究は雌ラットを用い、哺乳類メスに共通する発情行動の発現を担う神経内分泌機構を明らかにすることを目的とする。本研究の達成は、学術的な意義が高いだけでなく、本研究により明らかとなった因子に焦点をあてた育種選抜の基準開発に資するなど畜産分野に貢献を果たす基礎的知見を提供できる。

Outline of Final Research Achievements

In this study, I aimed to unravel the neural mechanism governing estrous behavior in female rats. To achieve this, I focused on identifying potential factors responsible for regulating estrous behavior by conducting an analysis of genes associated with estrogen receptor α (ERα) in the ventromedial hypothalamus, a crucial nucleus involved in the control of estrous behavior. Among the various candidates, the gene encoding neuropeptide B (NPB) emerged as the most candidate. Utilizing the GONAD technique, we successfully obtained neuropeptide B receptor 1 (Npbwr1) knockout candidate rats (F0) from five pregnant rats, then seven rats exhibiting the desired genetic modification.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ニューロペプチドB(NPB)の脳室内投与は、発情行動制御に関連のあるホルモン、黄体形成ホルモンやプロラクチン分泌の分泌を亢進することが明らかとなった。この結果は、生殖機能の中枢制御機構の全容解明への一助となりうる。また、本実験では、発情行動発現を制御する因子の同定にまでは至らなかったが、本研究で作出したNpbwr1遺伝子欠損ラットは、今後、NPB-NPBWR1シグナリングが発情行動に及ぼす影響を明らかにするために用いるだけでなく、摂食機能や生殖機能などの生理機能におけるNPB/NPW-Npbwr1の役割を詳細に解明するために有用なツールとなることが期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2023 2022 2021 2020 2019

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (4 results) Book (1 results)

  • [Journal Article] Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons2023

    • Author(s)
      Minabe Shiori、Iwata Kinuyo、Watanabe Youki、Ishii Hirotaka、Ozawa Hitoshi
    • Journal Title

      Endocrine Connections

      Volume: 12 Issue: 1

    • DOI

      10.1530/ec-22-0307

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphological Analysis of the Hindbrain Glucose Sensor-Hypothalamic Neural Pathway Activated by Hindbrain Glucoprivation2021

    • Author(s)
      Sato Marimo、Minabe Shiori、Sakono Takahiro、Magata Fumie、Nakamura Sho、Watanabe Youki、Inoue Naoko、Uenoyama Yoshihisa、Tsukamura Hiroko、Matsuda Fuko
    • Journal Title

      Endocrinology

      Volume: 162 Issue: 9 Pages: 1-18

    • DOI

      10.1210/endocr/bqab125

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mating-induced increase in <i>Kiss1</i> mRNA expression in the anteroventral periventricular nucleus prior to an increase in LH and testosterone release in male rats2020

    • Author(s)
      Watanabe Y., Ikegami K., Nakamura Sho., Uenoyama Y., Ozawa H., Maeda K., Tsukamura H., Inoue N.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 66 Issue: 6 Pages: 579-586

    • DOI

      10.1262/jrd.2020-067

    • NAID

      130007958759

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Testosterone supplementation rescues spermatogenesis and in vitro fertilizing ability of sperm in Kiss1 knockout mice.2020

    • Author(s)
      T. Goto, M. Hirabayashi, Y. Watanabe, M. Sanbo, K. Tomita, N. Inoue, H. Tsukamura, Y. Uenoyama.
    • Journal Title

      Endocrinology

      Volume: - Issue: 9 Pages: 1-11

    • DOI

      10.1210/endocr/bqaa092

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] GnRH(1-5), a metabolite of gonadotropin-releasing hormone, enhances luteinizing hormone release <i>via</i> activation of kisspeptin neurons in female rats2020

    • Author(s)
      Ieda N, Assadullah, Minabe S, Ikegami K, Watanabe Y, Sugimoto Y, Sugimoto A, Kawai N, Ishii H, Inoue N, Uenoyama Y, Tsukamura H
    • Journal Title

      Endocrine Journal

      Volume: 67 Issue: 4 Pages: 409-418

    • DOI

      10.1507/endocrj.EJ19-0444

    • NAID

      130007837043

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Conditional Kisspeptin Neuron-Specific Kiss1 Knockout With Newly Generated Kiss1-floxed and Kiss1-Cre Mice Replicates a Hypogonadal Phenotype of Global Kiss1 Knockout Mice2020

    • Author(s)
      Kana Ikegami, Teppei Goto, Sho Nakamura, Youki Watanabe, Arisa Sugimoto, Sutisa Majarune, Kei Horihata, Mayuko Nagae, Junko Tomikawa, Takuya Imamura, Makoto Sanbo, Masumi Hirabayashi, Naoko Inoue, Kei-Ichiro Maeda, Hiroko Tsukamura, Yoshihisa Uenoyama
    • Journal Title

      J Reprod Dev.

      Volume: in press Issue: 4 Pages: 359-367

    • DOI

      10.1262/jrd.2020-026

    • NAID

      130007888736

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Conditional Kisspeptin Neuron-Specific Kiss1 Knockout With Newly Generated Kiss1-floxed and Kiss1-Cre Mice Replicates a Hypogonadal Phenotype of Global Kiss1 Knockout Mice2020

    • Author(s)
      Kana Ikegami, Teppei Goto, Sho Nakamura, Youki Watanabe, Arisa Sugimoto, Sutisa Majarune, Kei Horihata, Mayuko Nagae, Junko Tomikawa, Takuya Imamura, Makoto Sanbo, Masumi Hirabayashi, Naoko Inoue, Kei-Ichiro Maeda, Hiroko Tsukamura, Yoshihisa Uenoyama
    • Journal Title

      Journal of reproduction and development

      Volume: -

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neonatal Estrogen Causes Irreversible Male Infertility via Specific Suppressive Action on Hypothalamic Kiss1 Neurons2019

    • Author(s)
      Minabe Shiori、Sato Marimo、Inoue Naoko、Watanabe Youki、Magata Fumie、Matsuda Fuko、Uenoyama Yoshihisa、Ozawa Hitoshi、Tsukamura Hiroko
    • Journal Title

      Endocrinology

      Volume: 160 Issue: 5 Pages: 1223-1233

    • DOI

      10.1210/en.2018-00732

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 泌乳期ラット視床下部におけるニューロペプチドWの発現2022

    • Author(s)
      原優奈、小野寺諒馬、中尾 暢宏、渡辺雄貴
    • Organizer
      日本繁殖生物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Neuropeptide Bの脳室内投与による黄体形成ホルモンの分泌増加作用2021

    • Author(s)
      渡辺雄貴、岩田衣世、小澤一史
    • Organizer
      日本繁殖生物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] ニューロペプチドBがメスラットのプロラクチン分泌に及ぼす影響2019

    • Author(s)
      渡辺雄貴、美辺詩織、岩田衣世、小澤一史
    • Organizer
      日本繁殖生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] メスラットのプロラクチン分泌に及ぼす エストロゲン非依存的なニューロペプチドBの効果2019

    • Author(s)
      渡辺雄貴、美辺詩織、岩田衣世、小澤一史
    • Organizer
      第46回日本神経内分泌学会
    • Related Report
      2019 Research-status Report
  • [Book] 原書13版 デュークス獣医生理学2020

    • Author(s)
      総監修者 鈴木浩悦
    • Total Pages
      906
    • Publisher
      学窓社
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2025-01-30  

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