| Project/Area Number |
19K16098
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shibayama Youtaro 国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (60815819)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | RNA localization / long noncoding RNA / Gene expression / Subcellular localization / subcellular localization / H3K27ac / lncRNA / CAGE sequencing / smFISH / noncoding RNA / chromatin / cell fractionation / RNA sequencing / RNA localisation / RNA displacement / cell fixation / in-situ digestion / biotinylation of oligos / in-situ hybridization / in-situ ligation / streptavidin pull-down / RNA-seq / transcription / gene regulation |
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| Outline of Research at the Start |
Most of the human genome do not code for any protein yet give rise to RNA products. The purpose of this study is to develop a new technology that will help us understand what most of these RNAs are doing in the human cell. This is an endeavour of fundamental biology with implications in diseases.
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| Outline of Final Research Achievements |
Our bodies function properly when our genes function as intended by nature. Proper functioning of genes is controlled at many levels. One of these is ensuring that RNA, molecules which carry information contained in genes, travel to proper locations inside our cells. I investigated where RNA from every single gene occurs in the human cell and how that changes when the cell is stressed from various stimulations. Where most RNAs keep their proper localization in stressed conditions, I identified a group of RNAs which do not. Remarkably, different kinds of stress induce different members of RNA to change localization, and in some cases, induce the same members of RNA to change localization in different patterns. Upon many classes of RNA that exist in our genome, a group of RNA called long non-coding RNA were the most dominant among those RNA that change localization upon stress. With a chromatin-bound RNA, I have shown that change in localization has functional relevance for the cell.
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| Academic Significance and Societal Importance of the Research Achievements |
One way by which genes function properly is by ensuring RNA travels to intended locations within the cell, a mechanism with important implications in health and disease. My results suggest a possible underlying reason for some diseases and may also provide a point of intervention.
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