Towards the elucidation of factors affecting the reproductive modes of the planarian Dugesia ryukyuensis.
Project/Area Number |
19K16175
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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Research Institution | Keio University (2021-2022) Hirosaki University (2019-2020) |
Principal Investigator |
SEKII Kiyono 慶應義塾大学, 商学部(日吉), 助教 (50786358)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | プラナリア / 生殖様式 / 共生細菌 |
Outline of Research at the Start |
扁形動物プラナリアには、無性生殖と有性生殖を使い分けるものがいる。この生殖様式の転換を可能にしているのが、プラナリアが全身に有する分化多能性幹細胞であり、ここから生殖細胞を含めたすべての組織を形成できる。しかし無性個体は分裂後、脳も含めてすべて再生するのに、生殖細胞・器官だけは作らず、無性状態を維持し続ける。このプラナリアが無性状態のままでいる仕組みに、最近、共生細菌が関与している可能性が示された。本研究では、この宿主であるプラナリアの無性状態に関与する細菌の存在・機能を明らかにする。
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Outline of Final Research Achievements |
Some flatworms can reproduce both asexually and sexually, and switch their reproductive modes based on environmental changes, life cycle stages, or both. However, the mechanisms by which they switch between asexual and sexual reproductive modes are not well understood. In the planarian Dugesia ryukyuensis, an experimental system has been established to convert asexually reproducing worms into sexual worms (sexualization), by feeding them sexually mature planarians. This feeding assay system enables us to study the factor that suppresses germ cell differentiation and keeps planarians in an asexual state. In the current study, I investigated the factors involved in the mechanism that maintains the asexual state, by using the feeding assay system, fluorescence in situ hybridization, and genomic analysis.
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Academic Significance and Societal Importance of the Research Achievements |
ボルバキアのように、宿主のオスとメスの比を自分の都合良く操作する共生細菌の存在はすでに知られているが、無性・有性という宿主の生殖様式を変えられる共生細菌の存在はこれまでまだ報告がない。受精卵を経て次世代をのこす有性生殖にくらべ、分裂・再生によって数を増やすプラナリアの無性生殖は共生細菌の増殖にとっても有利となり、そのように自分の利益のために宿主の生殖様式を操作する共生細菌は興味深い報告となる。
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Sex-Inducing Activities of the Land Planarian Bipalium nobile Extract Fractions, Obtained Using Bioassay-Guided Fractionation, in the Freshwater Planarian Dugesia ryukyuensis2021
Author(s)
Makoto Kitamura, Hiroyuki Tanaka, Yurie Horiguchi, Sayaka Manta, Ikuma Saito, Hisashi Iwaya, Hikaru Okamoto, Nanna Nagao, Yumi Yanagihara, Yu Taguchi, Rei Tezuka, Takanobu Maezawa, Kiyono Sekii, Kazuya Kobayashi
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Journal Title
Zoological science
Volume: 38
Issue: 6
Pages: 544-557
DOI
NAID
Related Report
Peer Reviewed
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