Parallel evolution of complex adaptations through introgression: the study case of pelvic fin brooding in Sulawesi medaka
| Project/Area Number |
19K16203
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 45020:Evolutionary biology-related
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| Research Institution | Japan Agency for Marine-Earth Science and Technology (2020-2021)
University of the Ryukyus (2019) |
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Principal Investigator |
Montenegro Javier 国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(超先鋭技術開発プログラム), 特任研究員 (70835347)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Complex traits / QTLs / Oryzias / Multiple loci / Pelvic-fin brooding / Evolution / Admixture / New Species / Sex Chromosome / QTL / Medaka / Complex trait / Introgression / Genome / genome / Adrianichthys / Sulawesi / Ancestral polymorphism / Convergent evolution / ddRAD-seq / Whole-genome / hybridization |
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| Outline of Research at the Start |
Complex adaptations are not expected to evolve rapidly. However, pelvic-fin brooding a reproductive mode of medaka has evolved in two distant lineages in Sulawesi. I hypothesize that this event of parallel evolution reflects historical hybridization between lineages.
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| Outline of Final Research Achievements |
Our analyzes revealed that females of pelvic-fin brooders have longer pelvic fins and a deeper abdominal concavity, and that they can carry eggs for longer than non-brooding species. QTL analyses revealed that pelvic-fin brooding is controlled by multiple and unliked loci. Genome wide comparative analyses detected no signatures of admixture between the two lineages of pelvic-fin brooders. A previously unknown species of pelvic-fin brooder Oryzias was discover from central Sulawesi. Phylogenetic analyses reveled that the new species belongs to the same clades as other pelvic fin brooders in genus Oryzias. A pure strain of O. eversi was rear. All F1 individuals were ddRAD sequenced. A single SNP was found to be perfectly associated, heterozygote
for all males and homozygotes for all females.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、メダカの腹鰭飼育をモデル系として、複雑な適応のゲノム構造を調べ、複雑な形質が種の系統を越えて水平移動しうるかどうかを評価した。近い将来、本研究で明らかになった遺伝子座をもとに、骨盤鰭繁殖に関連する個々の遺伝子をノックアウトしたり、非繁殖種を用いてトランスジェニック個体を作製することが可能になると考えられる。このような遺伝子工学的アプローチは、複雑な形質の進化に関する仮説を実験的に検証するユニークな機会を提供するものである。このように、本研究の成果は、確かな経験的証拠と進化仮説を立て検証するためのモデルシステムを提供することで、複雑な適応の進化の分野に影響を与えることが期待される。
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Report
(4 results)
Research Products
(2 results)
