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Population genomics of medaka: analysis of selection pressures in the wild

Research Project

Project/Area Number 19K16232
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Fujimoto Shingo  琉球大学, 医学(系)研究科(研究院), 特命研究員 (60795102)

Project Period (FY) 2019-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生活史適応 / 季節性 / 緯度間変異 / 自然淘汰 / メダカ / 集団遺伝解析 / ゲノムスキャン / 量的形質 / 緯度 / Oryzias latipes / 繁殖 / 行動生態 / 個体群動態 / 進化 / 集団遺伝
Outline of Research at the Start

成長や繁殖といった生活史特性は同一種内でも集団間で遺伝的に異なる。それぞれの環境では異なる種類の自然淘汰が働いて適応進化が生じているだろう。生活史の集団間変異が知られるメダカを対象に、生活史の適応進化に関わる遺伝子を探索する。複数の野生集団から全ゲノムの塩基配列を決定して変異カタログを作成する。そして、集団遺伝学的な指標を用いて遺伝子座ごとにどのような種類の淘汰メカニズムが働いたか分析する。集団遺伝指標で得られた遺伝子領域を、生活史に関する量的形質遺伝子座(QTL)の解析で得られた候補遺伝子との対応を明らかにして、集団間変異をもたらす淘汰の実体を明らかにする。

Outline of Final Research Achievements

In the wild medaka population, the reproductive seasonality changes along the latitude, and the population at high-latitude tend to be a shorter time of the reproductive season. The demographic changes may be achieved by adaptive evolution in life histories, such as growth and reproduction. We reported the reproductive pattern of the wild medaka population distributed in the Aomori where is near the northern limit of their distribution. In order to explore the genetic basis for inter-population variation in the life history of medaka populations, we analyzed the quantitative trait loci in F2 individuals crossed from wild individuals in Okinawa and Aomori. Whole-genome re-sequence were also performed on 6 populations at different latitudes. To detect footprints of natural selection, Fst and Tajima's D were calculated by performing genome scans at 50000 bp intervals.

Academic Significance and Societal Importance of the Research Achievements

野生動物における生活史の適応進化は主に表現型変異に基づいて研究されてきた。近年、ゲノムワイドな遺伝的変異の検出が容易になったことで分子メカニズムも検討できるようになりつつある。メダカ科魚類は熱帯に分布する近縁種を含めたゲノム解読を日本の研究チームが精力的に進めているため、日本のメダカは生活史の適応進化とその遺伝基盤を検証する有効な系である。しかしながら、野外での適応進化を議論するのに必要な野生集団の核ゲノムの対立遺伝子頻度に関する知見が限られることに課題があった。本研究で得られた野生集団の遺伝子変異カタログは生活史に限らず、野外での魚類の適応進化を研究する上で有効な基礎的知見になるだろう。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (15 results)

All 2021 2020 2019 Other

All Int'l Joint Research (5 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (5 results) Remarks (1 results)

  • [Int'l Joint Research] LIPI/Sam Ratulangi University/Museum Zoologicum Bogoriense(インドネシア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Hanoi National University of Education(ベトナム)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Living Aquatic Resources Research Center(ラオス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] MONREC(ミャンマー)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Kerala University(インド)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Seasonal change in male reproductive investment of a fish2021

    • Author(s)
      Fujimoto Shingo、Takeda Satoshi、Yagi Mitsuharu、Yamahira Kazunori
    • Journal Title

      Environmental Biology of Fishes

      Volume: 104 Issue: 2 Pages: 107-118

    • DOI

      10.1007/s10641-021-01059-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome editing reveals fitness effects of a gene for sexual dichromatism in Sulawesian fishes2021

    • Author(s)
      Ansai Satoshi、Mochida Koji、Fujimoto Shingo、Mokodongan Daniel F.、Sumarto Bayu Kreshna Adhitya、Masengi Kawilarang W. A.、Hadiaty Renny K.、Nagano Atsushi J.、Toyoda Atsushi、Naruse Kiyoshi、Yamahira Kazunori、Kitano Jun
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 1350-1350

    • DOI

      10.1038/s41467-021-21697-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Latitudinal variation in sexual dimorphism in a freshwater fish group2020

    • Author(s)
      Sumarto Bayu K A、Kobayashi Hirozumi、Kakioka Ryo、Tanaka Rieko、Maeda Ken、Tran Hau D、Koizumi Noriyuki、Morioka Shinsuke、Bounsong Vongvichith、Watanabe Katsutoshi、Musikasinthorn Prachya、Tun Sein、Yun L K C、Anoop V K、Raghavan Rajeev、Masengi Kawilarang W A、Fujimoto Shingo、Yamahira Kazunori
    • Journal Title

      Biological Journal of the Linnean Society

      Volume: 131 Issue: 4 Pages: 898-908

    • DOI

      10.1093/biolinnean/blaa166

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evidence for sympatric speciation in a Wallacean ancient lake2019

    • Author(s)
      Sutra Nobu、Kusumi Junko、Montenegro Javier、Kobayashi Hirozumi、Fujimoto Shingo、Masengi Kawilarang W. A.、Nagano Atsushi J.、Toyoda Atsushi、Matsunami Masatoshi、Kimura Ryosuke、Yamahira Kazunori
    • Journal Title

      Evolution

      Volume: 73 Issue: 9 Pages: 1898-1915

    • DOI

      10.1111/evo.13821

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 全ゲノム解析によるメダカ野生集団の過去の有効集団サイズ変動の推定2021

    • Author(s)
      藤本真悟, 木村亮介
    • Organizer
      第68回日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Latitudinal Variation in Sexual Dimorphisms in a Freshwater Fish Group2021

    • Author(s)
      Sumarto,B.K.A., Fujimoto,S., Kobayashi,H, Yamahira,K.
    • Organizer
      第68回日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] メダカ野生集団における季節性繁殖に関連する量的形質遺伝子座の探索2020

    • Author(s)
      藤本真悟,北野潤,山平寿智
    • Organizer
      第39回日本動物行動学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 長崎県平戸産ミナミメダカ個体群におけるRetrotransposonの挿入による新規性決定遺伝子GsdfNeoYへの進化2020

    • Author(s)
      明正大純,藤本真悟,小林亨
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 気候適応がもたらす繁殖の季節性がメダカ科魚類の性淘汰圧を変える2019

    • Author(s)
      藤本真悟
    • Organizer
      日本生態学会
    • Related Report
      2019 Research-status Report
  • [Remarks] 時空間ゲノミクス

    • URL

      http://w3.u-ryukyu.ac.jp/anthropology/jikuukan/

    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2022-01-27  

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