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Three-dimensional analysis of spermatogenesis disorder

Research Project

Project/Area Number 19K16473
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 48010:Anatomy-related
Research InstitutionKanazawa University

Principal Investigator

Nakata Hiroki  金沢大学, 医学系, 講師 (80638304)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords精巣 / 精細管 / 三次元 / 三次元再構築 / 3D / 精子形成 / ブスルファン / 加齢 / 精子形成障害 / マウス
Outline of Research at the Start

申請者が考案した ”三次元再構築精巣” と ”三次元マッピング” という新たな研究ツールと、男性不妊症のヒト精巣と同じ状態になるモデル動物を用いることで、障害が精巣内のどの場所から、どのような変化として始まり、どのように進展していくかを明らかにする。また、三次元再構築精巣に血管および各種マーカー情報をマッピングすることで、精子形成障害の発生と進展に関連する因子を同定する。

Outline of Final Research Achievements

We prepared mice with different degrees of spermatogenesis disorder by changing the doses of busulfan injection and different aged mice. Then, the 3D-reconstructed seminiferous tubules were analyzed to examine if the impaired or normal spermatogenesis was distributed preferentially in particular sites in seminiferous tubules or in the testis. There were 3D positions with the specific proportions common to different testes not in busulfan-induced mice but in aged mice.

Academic Significance and Societal Importance of the Research Achievements

薬剤または加齢という全く異なる要因において、正常または精子形成障害が起こりやすい三次元部位がそれぞれ明らかになった。本研究で得られた成果は、精子形成障害を研究する上で基礎的なデータとなり、男性の妊孕性・不妊症の研究の発展に寄与できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (18 results)

All 2022 2021 2020 2019

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Comparative anatomy on the development of sperm transporting pathway between the testis and mesonephros2022

    • Author(s)
      Omotehara Takuya、Nakata Hiroki、Nagahori Kenta、Itoh Masahiro
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 157 Issue: 3 Pages: 321-332

    • DOI

      10.1007/s00418-021-02057-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three‐dimensional structure of testis cords in mice and rats2021

    • Author(s)
      Nakata Hiroki、Omotehara Takuya、Itoh Masahiro、Iseki Shoichi、Mizokami Atsushi
    • Journal Title

      Andrology

      Volume: 9 Issue: 6 Pages: 1911-1922

    • DOI

      10.1111/andr.13069

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three‐dimensional reconstruction of testis cords/seminiferous tubules2021

    • Author(s)
      Nakata Hiroki、Iseki Shoichi、Mizokami Atsushi
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 20 Issue: 4 Pages: 402-409

    • DOI

      10.1002/rmb2.12413

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional morphological analysis of spermatogenesis in aged mouse testes2021

    • Author(s)
      Nakano Taito、Nakata Hiroki、Kadomoto Suguru、Iwamoto Hiroaki、Yaegashi Hiroshi、Iijima Masashi、Kawaguchi Shohei、Nohara Takahiro、Shigehara Kazuyoshi、Izumi Kouji、Kadono Yoshifumi、Mizokami Atsushi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41598-021-02443-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three‐dimensional analysis of mesonephric tubules remodeling into efferent tubules in the male mouse embryo2021

    • Author(s)
      Omotehara Takuya、Nakata Hiroki、Itoh Masahiro
    • Journal Title

      Developmental Dynamics

      Volume: 251 Issue: 3 Pages: 513-524

    • DOI

      10.1002/dvdy.410

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three‐dimensional structure of seminiferous tubules in the Syrian hamster2020

    • Author(s)
      Nakata Hiroki、Yoshiike Miki、Nozawa Shiari、Sato Yoko、Iseki Shoichi、Iwamoto Teruaki、Mizokami Atsushi
    • Journal Title

      Journal of Anatomy

      Volume: 238 Issue: 1 Pages: 86-95

    • DOI

      10.1111/joa.13287

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-Dimensional Analysis of Busulfan-Induced Spermatogenesis Disorder in Mice2020

    • Author(s)
      Nakata Hiroki、Nakano Taito、Iseki Shoichi、Mizokami Atsushi
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 609278-609278

    • DOI

      10.3389/fcell.2020.609278

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マウス・ラット精巣索の3次元構造2022

    • Author(s)
      仲田浩規, 表原拓也, 伊藤正裕, 井関尚一, 溝上敦
    • Organizer
      第127回日本解剖学会総会・全国学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 精巣と精子形成障害の三次元解析2021

    • Author(s)
      仲田浩規
    • Organizer
      第31回精子形成・精巣毒性研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] マウス・ラット精巣索の3次元構造2021

    • Author(s)
      仲田浩規, 表原拓也, 伊藤正裕, 溝上敦
    • Organizer
      日本アンドロロジー学会第40回学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] マウス・ラット精巣索の3次元構造2021

    • Author(s)
      仲田浩規, 表原拓也, 伊藤正裕, 井関尚一, 溝上敦
    • Organizer
      第81回日本解剖学会中部支部学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 精子形成障害モデルマウスの三次元解析2021

    • Author(s)
      仲田浩規, 溝上敦
    • Organizer
      第109回日本泌尿器科学会総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] マウス精巣外精路の三次元構造2020

    • Author(s)
      仲田浩規
    • Organizer
      第80回日本解剖学会中部支部学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] マウス精巣輸出管・上体管の三次元構造2020

    • Author(s)
      仲田浩規、吉池美紀、野澤資亜利、佐藤陽子、井関尚一、岩本晃明、溝上敦
    • Organizer
      日本アンドロロジー学会第39回学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ハムスター精細管の三次元構造2020

    • Author(s)
      仲田浩規、溝上敦
    • Organizer
      第108回日本泌尿器科学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] ハムスター精細管の三次元構造2020

    • Author(s)
      仲田浩規
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] マウス精巣外精路の三次元構造2019

    • Author(s)
      仲田浩規
    • Organizer
      日本アンドロロジー学会第38回学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Three-dimensional structure of mouse seminiferous tubules2019

    • Author(s)
      仲田浩規
    • Organizer
      69th Annual Meeting of Korean Association of Anatomist
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2019-04-18   Modified: 2023-01-30  

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