Analysis of amyloid-associated proteins by imaging mass spectrometry using FFPE tissue
Project/Area Number |
19K16561
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 49020:Human pathology-related
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | アミロイド / プロテオミクス / FFPE / アミロイドーシス / IMS / イメージング質量分析 |
Outline of Research at the Start |
本研究では、イメージング質量分析(IMS)技術を基盤とした、アミロイドーシスで異常蓄積する原因蛋白質の同定法、アミロイドーシス病型診断法の確立を目指す。そのために、アミロイドーシスのホルマリン固定パラフィン包埋(formalin fixed paraffin embedded; FFPE)組織を用い、LC-MS/MSによる比較プロテオミクスとIMSを行ない、アミロイドーシスの病型ごとに、得られたペプチドのマススペクトルをデータベース化する。最終的に、IMSデータのパターン分類のみによるアミロイドーシス病型診断システムを構築する。
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Outline of Final Research Achievements |
We aimed to identify amyloidosis-causing proteins by imaging mass spectrometry (IMS) using FFPE tissue. First, a comparative proteomic analysis was performed in hearts, in amyloidosis cases, and in target cases. A total of 2324 proteins were identified. Multivariate analysis using 678 proteins, which were significantly increased in amyloidosis cases, showed that amyloidosis and control cases were clearly divided into groups. Furthermore, Tissue Microarray slides were prepared for 12 proteins uniquely expressed in amyloidosis cases, and IMS targeting specific MS spectra identified promising peaks.
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Academic Significance and Societal Importance of the Research Achievements |
本研究は、近年増加傾向にあり、新規治療薬が登場しているアミロイドーシス患者の早期診断、適切な治療法選択に大いに寄与し、有望かつ有効な治療戦略創出のための重要な知見となりうる。本研究により得られる知見の蓄積と発信は、IMSによる in situ proteome analysis とでも言うべき、全く新しい次元の病態理解、診断技術開発への嚆矢となる。また、研究を進める中で、原因物質不明のアミロイドーシス症例をプロテオーム解析することで、最近になり報告された全身性APOA4アミロイドーシスであることが判明し、論文として報告している。アミロイドーシスにおけるプロテオーム解析の有用性が示唆された。
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Aberrant expression of junctional adhesion molecule-A contributes to the malignancy of cervical adenocarcinoma by interaction with poliovirus receptor/CD155.2021
Author(s)
Murakami T, Takasawa A, Takasawa K, Akimoto T, Aoyama T, Magara K, Saito Y, Ota M, Kyuno D, Yamamoto S, Hasegawa T, Saito T, Osanai M.
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Journal Title
Cancer Science
Volume: 112
Issue: 2
Pages: 906-917
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Aldolase A promotes epithelial-mesenchymal transition to increase malignant potentials of cervical adenocarcinoma.2020
Author(s)
Saito Y, Takasawa A, Takasawa K, Aoyama T, Akimoto T, Ota M, Magara K, Murata M, Hirohashi H, Hasegawa T, Sawada N, Saito T, Osanai M.
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Journal Title
Cancer Science
Volume: 111
Issue: 8
Pages: 3071-3081
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Molecular Aberrations Associated with Seizure Control in Diffuse Astrocytic and Oligodendroglial Tumors2020
Author(s)
Suzuki H, Mikuni N, Sugita S, Aoyama T, Yokoyama R, Suzuki Y, Enatsu R, Akiyama Y, Mikami T, Wanibuchi M, Hasegawa T.
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Journal Title
Neurologia medico-chirurgica
Volume: 60
Issue: 3
Pages: 147-155
DOI
NAID
ISSN
0470-8105, 1349-8029
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of Coiled-Coil Domain-Containing Protein 180 and Leucine-Rich Repeat-Containing Protein 4 as Potential Immunohistochemical Markers for Liposarcoma Based on Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded Tissue.2019
Author(s)
Aoyama T, Takasawa A, Takasawa K, Ono Y, Emori M, Murata M, Hayasaka T, Fujitani N, Osanai M, Yamashita T, Hasegawa T, Sawada N.
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Journal Title
Am J Pathol.
Volume: in press
Issue: 5
Pages: 1015-1028
DOI
Related Report
Peer Reviewed
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[Presentation] Aberrant high expression of ALDOA contributes to the malignant transformation of uterine cervical adenocarcinoma2021
Author(s)
Akira takasawa, Yuki saito, Kumi Takasawa, Taishi Akimoto, Kazufumi Magara, Tomoyuki Aoyama, Yusuke Ono, Daisuke Kyuno, Yoshihiko Hirohashi, Tsuyoshi Saito, Makoto Osanai
Organizer
The 39 Sapporo International Cancer Symposium
Related Report
Int'l Joint Research
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