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the regulation of IgE production by follicular dendritic cells

Research Project

Project/Area Number 19K16608
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 49030:Experimental pathology-related
Research InstitutionUniversity of Toyama (2022)
Mie University (2019-2021)

Principal Investigator

Kuniishi Mari  富山大学, 学術研究部薬学・和漢系, 助教 (70804326)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsFDC / Antibody / allergy / isolation / Ab production / IgA / B cell / リンパ節 / stromal cell / 支持細胞 / IgE / リンパ組織 / I型アレルギー / 抗原抗体複合体
Outline of Research at the Start

I型アレルギー疾患では、IgE抗体が増加しアレルギー反応の惹起に寄与すると考えられている。IgE抗体はFollicular dendritic cell(FDC)が局在する胚中心でB細胞より産生される。FDCは非造血系細胞でB細胞の活性化を促す一方で、IgE抗体の産生を制御することも知られている。しかし、様々なリンパ組織で機能や発現分子等が異なるとの報告から、一様にIgE抗体を制御できるか詳細は不明である。そこで、本研究では各リンパ組織でのFDCがIgE抗体産生を制御しうるか検討し、IgE産生のメカニズムをリンパ組織の微小環境の観点から解明することを目指す。

Outline of Final Research Achievements

In this study, we performed experiments to examine the effects of follicular dendritic cells (FDCs) in lymph nodes on the production of IgE antibodies and other antibodies. As a result, we established a method to isolate FDCs efficiently and to evaluate the function of FDCs in co-culture with B cells. Furthermore, we found that FDCs in each lymph node promote the production of IgA antibodies, although not IgE antibodies. We suggest that FDCs of mucosal tissues as well as FDCs of non-mucosal tissues might promote IgA antibody production if the environment is favorable.

Academic Significance and Societal Importance of the Research Achievements

FDCは免疫細胞や他の非造血系細胞に比べて単離が難しく、他の細胞の影響を考えることなく、B細胞への直接的な影響についてはほとんど分かっていない。本研究成果は、FDCの機能を解析できる系を確立したことにより、FDCの抗体産生に与える機能の解析が可能になった点で、学術的意義は大きいと考える。また、現在免疫反応は、非造血系細胞が作りだす環境が血液細胞の機能を促進する上で重要なことが分かってきている。本研究は、様々な疾患で誘導される免疫反応がどのように非造血系細胞によって制御されるかその基礎的研究となり、社会的意義も大きいと考える。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (15 results)

All 2023 2022 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (11 results)

  • [Journal Article] Role of tumor necrosis factor receptor-associated factor 5 in B- and T-lymphocytes2023

    • Author(s)
      Hikosaka Kuniishi Mari、Ishii Naoto、So Takanori
    • Journal Title

      Exploration of Immunology

      Volume: 3 Pages: 40-55

    • DOI

      10.37349/ei.2023.00088

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of CD35+ follicular dendritic cells and its role in the differentiation from B cells to IgA+GL7+ cells2022

    • Author(s)
      Hikosaka-Kuniishi M, Yamane T, Isono K, Tetteh DN, Yamazaki H
    • Journal Title

      Immunology Letters

      Volume: 243 Pages: 53-60

    • DOI

      10.1016/j.imlet.2022.02.005

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multiple cell populations generate macrophage progenitors in the early yolk sac2022

    • Author(s)
      Ito C, Hikosaka-Kuniishi M, Yamazaki H, Yamane T
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 79 Issue: 3 Pages: 159-159

    • DOI

      10.1007/s00018-022-04203-7

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous Fluorescent Identification of Odontoblasts and Ameloblasts2021

    • Author(s)
      Isono K, Takahashi E, Miyoshi I, Tsuneto M, Hikosaka-Kuniishi M, Yamane T, Yamazaki H
    • Journal Title

      J. Dent. Res.

      Volume: 100 Issue: 5 Pages: 532-541

    • DOI

      10.1177/0022034520974576

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] The role of isolated CD35+ follicular dendritic cells in the differentiation from B cells to IgA+ GL7+ cells2022

    • Author(s)
      Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Doris Narki Tetteh, Hidetoshi Yamazaki
    • Organizer
      The 51st Annual Meeting of The Japanese Society for Immunology
    • Related Report
      2022 Annual Research Report
  • [Presentation] Neural crest-derived mesenchymal cells support thymic regeneration after lethal irradiation2022

    • Author(s)
      Doris Narki Tetteh, Hidetoshi Yamazaki, Mari Hikosaka-Kuniishi
    • Organizer
      The 51st Annual Meeting of The Japanese Society for Immunology
    • Related Report
      2022 Annual Research Report
  • [Presentation] Follicular dendritic cell-mediated enhancement of the differentiation into IgA+GL7+ cells2021

    • Author(s)
      Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      the 50th annual meeting of the japanese society for immunology
    • Related Report
      2021 Research-status Report
  • [Presentation] リンパ節ストローマ細胞Follicular dendritic cellによるレチノイン酸を介したIgA抗体制御機序の解明への試み2021

    • Author(s)
      國石(彦坂) 茉里、磯野 加奈、山崎 英俊
    • Organizer
      第2回 Winter Dental Meeting in Tsu(ウインターデンタルミーティングin津)
    • Related Report
      2021 Research-status Report
  • [Presentation] エナメル質形成不全マウス(Amelx♂)及び象牙質形成不全マウス(DsppGFP/GFP)を用いたエナメル質及び象牙質の構造解析の試み2021

    • Author(s)
      磯野 加奈、國石(彦坂) 茉里、山崎 英俊
    • Organizer
      第2回 Winter Dental Meeting in Tsu(ウインターデンタルミーティングin津)
    • Related Report
      2021 Research-status Report
  • [Presentation] リンパ組織支持細胞Follicular dendritic cellによる粘膜免疫に関わるIgA抗体制御機構解明の試み2020

    • Author(s)
      國石(彦坂)茉里、磯野加奈、山崎英俊
    • Organizer
      第1回Winter Dental meeting in 津(第48回三重歯科・口腔外科学会)
    • Related Report
      2020 Research-status Report
  • [Presentation] Amelx欠損及びDspp遺伝子欠損マウスの歯のCT解析2020

    • Author(s)
      磯野加奈、國石(彦坂)茉里、山崎英俊
    • Organizer
      第1回Winter Dental meeting in 津(第48回三重歯科・口腔外科学会)
    • Related Report
      2020 Research-status Report
  • [Presentation] Involvement of follicular dendritic cells in each LNs with IgAproduction2019

    • Author(s)
      Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      The 48th Annual Meeting of the Japanese Society for Immunology
    • Related Report
      2019 Research-status Report
  • [Presentation] Multiple cell populations generate macrophage progenitors in the early yolk sac2019

    • Author(s)
      Toshiyuki Yamane, Mari Hikosaka-Kuniishi, Hidetoshi Yamazaki
    • Organizer
      The 48th Annual Meeting of the Japanese Society for Immunology
    • Related Report
      2019 Research-status Report
  • [Presentation] The role of Neural crest-derived cells in thymic regeneration2019

    • Author(s)
      Doris Narki Tetteh, Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      The 48th Annual Meeting of the Japanese Society for Immunology
    • Related Report
      2019 Research-status Report
  • [Presentation] 粘膜免疫に関与するリンパ節支持細胞 follicular dendritic cellの検出の試み2019

    • Author(s)
      國石(彦坂)茉里, 磯野加奈, 山崎英俊
    • Organizer
      第47回 三重歯科・口腔外科学会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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