Role of kinesin/dynein adaptor JSAP in reactive oxygen species-induced cell death and lysosome positioning
| Project/Area Number |
19K16737
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 50010:Tumor biology-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
I.Ketut Gunarta 金沢大学, がん進展制御研究所, 助教 (90838393)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | JSAP / JLP / JSAP1 / Curcumin / Autophagy / Lysosome / JSAP2 / Cell death / Reactive oxygen species |
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| Outline of Research at the Start |
Cancer cell executes autophagy as a survival system under oxidative stress and this process is tightly linked to the position of the lysosome. In this study, I will investigate the relationship between JSAP-mediated lysosome trafficking and cell death under curcumin-induced oxidative stress.
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| Outline of Final Research Achievements |
The ROS level is tightly regulated by a cell as it can oxidize molecules inside a cell and ultimately lead to cell death. ROS inducing agent, such as curcumin, has been reported to alter lysosome localization in a cancer cell. We found that JSAP, a family of motor protein adaptors, is involved in lysosome transport in response to curcumin. We also clarified the relationship between JSAP-mediated lysosome transport and curcumin-induced cell death in cancer. Our study suggested that the closely related member of the JSAP family, JSAP1 and JSAP2 (also known as JLP), play a distinct function in curcumin-induced stress.
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| Academic Significance and Societal Importance of the Research Achievements |
ROSレベルを上昇させ、細胞死を誘発する治療薬は、代替の癌治療を提供します。 ウコン（Curcuma longa）に由来する化合物であるクルクミンは、インビトロおよびインビボで細胞死を誘発する有望な効果があるため、魅力的な薬剤候補です。 この研究の結果は、細胞内輸送が、新しい治療戦略の開発につながる可能性のある急性酸化ストレスの生存にどのように関連していたかについてのより良い理解を与えることが期待されます。
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] c-Jun NH<sub>2</sub>-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1) attenuates curcumin-induced cell death differently from its family member, JNK-associated leucine zipper protein (JLP)2021
Author(s)
I Ketut Gunarta, Dewi Yuliana, Purev Erdenebaatar, Yuhei Kishi, Jambaldorj Boldbaatar, Ryusuke Suzuki, Ravdandorj Odongoo, Gantulga Davaakhuu, Hirohiko Hohjoh, Katsuji Yoshioka
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Journal Title
Drug Discoveries & Therapeutics
Volume: 15
Issue: 2
Pages: 66-72
DOI
NAID
ISSN
1881-7831, 1881-784X
Year and Date
2021-04-30
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Peptidylarginine Deiminase 4 Promotes the Renal Infiltration of Neutrophils and Exacerbates the TLR7 Agonist-Induced Lupus Mice2020
Author(s)
Norio Hanata, Hirofumi Shoda, Hiroaki Hatano, Yasuo Nagafuchi, Toshihiko Komai, Tomohisa Okamura, Akari Suzuki, I Ketut Gunarta, Katsuji Yoshioka, Kazuhiko Yamamoto, Keishi Fujio
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Journal Title
Frontiers in immunology
Volume: 11
Pages: 1095-1095
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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