• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation of interaction enthalpy between water and myoproteins in skeletal muscle for theoretical interpretation of MR signals

Research Project

Project/Area Number 19K17180
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 52040:Radiological sciences-related
Research InstitutionJikei University School of Medicine

Principal Investigator

Nakahara Naoya  東京慈恵会医科大学, 医学部, 助教 (10632193)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsDSC / 水 / 骨格筋 / MR信号 / 筋タンパク
Outline of Research at the Start

MRIでは病気ごとに違う水の状態を利用して画像にしている。ただし、実際にはMRIでみる水の状態は同じようにみえても複数の解釈があり、経験を合わせて診断している。この水の状態をMRIとは違う、周囲の水分子との相互作用エネルギーの変化を反映する融解熱という観点でみるのが本研究である。実際細胞内には-20℃以下に融解熱をもつ水がある。サルコメアを基本構造として、規則周期的に構成されている骨格筋なら融解熱の局在を解明するのに適している。この-20℃以下に融解熱をもつ水が骨格筋細胞の中の具体的にどこにあるのか、この水はMRIでみている水の状態とどのような関係があるかを解明するのが、研究の目的である。

Outline of Final Research Achievements

Magnetic resonance images reflect not only water content but also water states in physiological tissue. Although water states in the tissue are not clarified yet in detail, interaction between water and macromolecules is generally considered to restrict motional freedom of the water molecules similarly to the freezing of water. As is the case with melting of ice, differential scanning calorimetry (DSC) can represent the restriction as extra heat absorption. DSC measurement with skinned fibers of the sartorius muscle from bull frogs at a rigor condition showed extra heat absorption at -24, -21, and 0 ℃. The extra heat absorption at -24, -21 ℃ reflected the intracellular states of actin and myosin. Integrated heat capacity in the range from -80℃ to 20℃ was decreased by denaturation or removal of actin. These results suggest that actin and myosin independently and cooperatively restrict surrounding water and that extra integrated heat capacity mainly depends on actin filaments.

Academic Significance and Societal Importance of the Research Achievements

従来のMR信号での解析では水分子の集団としてのふるまいを検出することは可能であったが、水分子間相互作用をエネルギーとしての評価をすることは困難であった。本研究で行ったDSCによる評価は水分子間相互作用をエネルギーとして評価するだけでなく、その水分子の局在まで明らかにできた。これらの研究を進めていくことで、生体内の水分子の相互作用エネルギーとMR信号の対応付けを行うことで、MR画像を理論的な解釈が可能なものにしていく一助になると考えられる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (8 results)

All 2022 2021 2020 2019

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (5 results)

  • [Journal Article] Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model2022

    • Author(s)
      Oya Ryohei、Tsukamoto Osamu、Hitsumoto Tatsuro、Nakahara Naoya、Okamoto Chisato、Matsuoka Ken、Kato Hisakazu、Inohara Hidenori、Takashima Seiji
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 3 Pages: 1747-1747

    • DOI

      10.3390/ijms23031747

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] X-ray Diffraction Analysis to Explore Molecular Traces of Eccentric Contraction on Rat Skeletal Muscle Parallelly Evaluated with Signal Protein Phosphorylation Levels2021

    • Author(s)
      Hirano Kazuhiro、Yamauchi Hideki、Nakahara Naoya、Kinoshita Kazuo、Yamaguchi Maki、Takemori Shigeru
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 23 Pages: 12644-12644

    • DOI

      10.3390/ijms222312644

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crossbridge Recruitment Capacity of Wild-Type and Hypertrophic Cardiomyopathy-Related Mutant Troponin-T Evaluated by X-ray Diffraction and Mechanical Study of Cardiac Skinned Fibers2020

    • Author(s)
      Yamaguchi Maki、Kimura Masako、Ohno Tetsuo、Nakahara Naoya、Akiyama Nobutake、Takemori Shigeru、Yagi Naoto
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 10 Pages: 3520-3520

    • DOI

      10.3390/ijms21103520

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] In vivo skeletal muscle x-ray diffraction study on the cause of reduced contractility after blood flow interruption2022

    • Author(s)
      中原直哉、山内秀樹、山口眞紀、林友則、平野和宏、竹森重
    • Organizer
      第99回日本生理学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development of x-ray diffraction methods for in vivo skeletal muscle with maintained blood supply2021

    • Author(s)
      中原直哉、山内秀樹、山口眞紀、平野和宏、竹森重
    • Organizer
      第126回日本解剖学会総会・全国学術集会、第98回日本生理学会大会 合同大会
    • Related Report
      2020 Research-status Report
  • [Presentation] X線回折法による血流を保った骨格筋の除神経後の変化2021

    • Author(s)
      中原直哉、山内秀樹、山口眞紀、平野和宏、竹森重
    • Organizer
      2020年度量子ビームサイエンスフェスタ
    • Related Report
      2020 Research-status Report
  • [Presentation] Actin filaments render considerable heat capacity to skeletal muscle sa陀omere2020

    • Author(s)
      中原直哉
    • Organizer
      日本生理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 熱測定で評価した骨格筋内の水と筋タンパクの相互作用2019

    • Author(s)
      中原直哉、大野哲生、木村雅子、木村澄子、竹森重
    • Organizer
      成医会
    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi