Generation of HIV-1 resistant T cells from induced pluripotent stem cell using CRISPR/Cas9 system
| Project/Area Number |
19K17925
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Osaka University (2021-2022)
Kobe University (2019) |
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Principal Investigator |
オフィンニ ユディル 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (30837901)
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| Project Period (FY) |
2021-03-01 – 2023-03-31
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| Project Status |
Discontinued (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | HIV-1 / iPSC / CRISPR/Cas9 / T cell / Feeder-free / Lentivirus |
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| Outline of Research at the Start |
HIV-1 infection is incurable. To cure HIV-1 infection, direct disruption of viral gene is necessary. We previously have developed anti-HIV gene editing using CRISPR/Cas9. To proceed into clinical application, usage of stem cells is the most promising. We plan to use induced pluripotent stem cells (iPSC) to generate large supply of T cells. Thus, our study plan is to efficiently develop iPSC containing anti-HIV-1 CRISPR/Cas9, and differentiate iPSC to generate HIV-1 resistant T cells. Successful generation may lead to a clinically applicable, complete cure for HIV-1.
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| Outline of Annual Research Achievements |
Step 1 of the study has been published in Viruses in 2020 and selected as a cover story of the journal's #11 issue. In Step 2, I have successfully transduced Cas9-gRNA into human mobilized hematopoietic stem cells (HSC) using a lentivector with Cocal virus pseudotyped-envelope. But, Step 3 did not go well as I moved to the US (HIV lab in Harvard), Osaka (Immunology), and now Kobe (Clinical Virology). The labs were not an iPSC lab. iPSC differentiation needed strict protocol and culture environment, and I did not succeed in differentiating the transduced iPSC. I am still hoping to establish a position in an iPSC lab to continue the project.
I prematurely ended the KAKENHI project as I was adopted as a Program-Specific Assistant Professor in Kobe and cannot have any extra research effort.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Monkeypox: A Comprehensive Review2022
Author(s)
Harapan Harapan、Ophinni Youdiil、Megawati Dewi、Frediansyah Andri、Mamada Sukamto S.、Salampe Mirnawati、Bin Emran Talha、Winardi Wira、Fathima Raisha、Sirinam Salin、Sittikul Pichamon、Stoian Ana M.、Nainu Firzan、Sallam Malik
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Journal Title
Viruses
Volume: 14
Issue: 10
Pages: 2155-2155
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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