• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of minimally invasive diagnostic method for malignant hyperthermia using fibroblasts

Research Project

Project/Area Number 19K18298
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 55050:Anesthesiology-related
Research InstitutionHiroshima University

Principal Investigator

Kondo Takashi  広島大学, 病院(医), 講師 (20711774)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords悪性高熱症
Outline of Research at the Start

悪性高熱症が疑われる患者に対して診断のための筋生検を行う際に、同時に皮膚から線維芽細胞を採取して培養し、筋芽細胞に分化誘導する遺伝子導入試薬を付加して骨格筋細胞に分化させて機能検査を行う。この診断法が確立すれば従来の検査よりも患者に与える侵襲を軽減することができ、検査が従来より普及することが期待される。また、患者血液の遺伝子情報を解析することで新たな原因遺伝子の同定や病態のさらなる解明も期待される。

Outline of Final Research Achievements

To perform a less invasive functional test for malignant hyperthermia (MH), which requires diagnosis by muscle biopsy, we use a transcription factor (MyoD1) that induces the differentiation of fibroblasts into skeletal muscle cells (myotube cells). We created myotube cells by introducing MyoD1 into skin fibroblasts. Next, a calcium sensitivity test was performed on myotube cells prepared from fibroblasts, and a comparison was made with a calcium susceptibility test on myotube cells in MH patients.
As a result of the examination, the calcium sensitivity of myotube cells derived from MH patients was significantly higher than that of myotube cells prepared from normal fibroblasts. The results of this study showed the possibility that myotube cells prepared from fibroblasts could be used for MH function testing.

Academic Significance and Societal Importance of the Research Achievements

悪性高熱症(MH)の機能診断のために必要な筋生検は侵襲が大きく、特に小児においては全身麻酔が必要となるため検査を行うことが困難な実態にある。
本研究成果により、正常ヒト線維芽細胞を分化させて作成した筋細胞によって機能解析を行うことができることが示された。現状では、MH患者の皮膚線維芽細胞から分化させた筋細胞を用いた機能解析は実用化されていないが、本研究の方法による皮膚を利用する検査法が確立すれば、従来の筋生検を必要とする検査よりも患者に与える侵襲を軽減することができ、MHの検査が従来よりも普及することが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (3 results)

  • [Journal Article] Malignant hyperthermia in a 16-day-old infant with congenital diaphragmatic hernia: a case report2021

    • Author(s)
      Tsutsumi YM, Kakuta N, Kawanishi R, Tanaka K, Kanzaki R, Morio A, Noda Y, Miyoshi H, Kondo T, Mukaida K.
    • Journal Title

      Journal of anesthesia

      Volume: 35 Issue: 2 Pages: 311-314

    • DOI

      10.1007/s00540-021-02902-2

    • NAID

      40022524264

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of blood pressure change during surgical incision under opioid analgesia using sympathetic response evoking threshold2021

    • Author(s)
      Kamiya Satoshi、Nakamura Ryuji、Saeki Noboru、Kondo Takashi、Miyoshi Hirotsugu、Narasaki Soushi、Morio Atsushi、Kawamoto Masashi、Hirano Harutoyo、Tsuji Toshio、Tsutsumi Yasuo M.
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 9558-9558

    • DOI

      10.1038/s41598-021-87636-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Leucine induces cardioprotection in vitro by promoting mitochondrial function via mTOR and Opa-1 signaling.2021

    • Author(s)
      Morio A, Tsutsumi R, Kondo T, Miyoshi H, Kato T, Narasaki S, Satomi S, Nakaya E, Kuroda M, Sakaue H, Kitamura T, Tsutsumi YM
    • Journal Title

      Nutr Metab Cardiovasc Dis

      Volume: 31 Issue: 10 Pages: 2979-2986

    • DOI

      10.1016/j.numecd.2021.06.025

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leucine imparts cardioprotective effects by enhancing mTOR activity and mitochondrial fusion in a myocardial ischemia/reperfusion injury murine model.2021

    • Author(s)
      Morio A, Tsutsumi R, Satomi S, Kondo T, Miyoshi H, Kato T, Kuroda M, Kitamura T, Hara K, Saeki N, Sakaue H, and Tsutsumi YM
    • Journal Title

      Diabetol Metab Syndr

      Volume: 13 Issue: 1 Pages: 39-39

    • DOI

      10.1186/s13098-021-00755-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraoperative responses of motor evoked potentials to the novel intravenous anesthetic remimazolam during spine surgery: a report of two cases2020

    • Author(s)
      Kondo Takashi、Toyota Yukari、Narasaki Soshi、Watanabe Tomoyuki、Miyoshi Hirotsugu、Saeki Noboru、Tsutsumi Yasuo M.
    • Journal Title

      JA Clinical Reports

      Volume: 6 Issue: 1 Pages: 97-97

    • DOI

      10.1186/s40981-020-00401-z

    • NAID

      120007169773

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of a new face mask concept for oxygen administration: a crossover study in healthy volunteers.2020

    • Author(s)
      Kondo T, Saeki N, Otsuki S, Tsutsumi YM.
    • Journal Title

      Journal of Anesthesia

      Volume: 34 Issue: 6 Pages: 950-952

    • DOI

      10.1007/s00540-020-02861-0

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of the Effects of Desflurane, Sevoflurane, and Propofol on the Glottic Opening Area during Remifentanil-Based General Anesthesia Using a Supraglottic Airway Device2020

    • Author(s)
      Kondo Takashi、Izumi Hiromichi、Kitagawa Makiko
    • Journal Title

      Anesthesiology Research and Practice

      Volume: 2020 Pages: 1-6

    • DOI

      10.1155/2020/1302898

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A case of delayed emergence after aortic root and ascending aorta replacement, which was probably caused by portosystemic shunt2019

    • Author(s)
      呉 晟名, 髙橋 信也, 森田 翔平, 前田 和樹, 片山 桂次郎, 黒崎 達也, 近藤 隆志, 村下 純二, 末田 泰二郎
    • Journal Title

      Cardiovascular Anesthesia

      Volume: 23 Issue: 1 Pages: 83-87

    • DOI

      10.11478/jscva.2019-3-001

    • NAID

      130007709369

    • ISSN
      1342-9132, 1884-7439
    • Year and Date
      2019-08-01
    • Related Report
      2019 Research-status Report
  • [Presentation] フォンタン術後患者の副腎外褐色細胞腫に対する後腹膜鏡下腫瘍摘除術の麻酔経験2020

    • Author(s)
      近藤隆志,竹野典子,神谷諭史,森尾篤,三好寛二,堤保夫
    • Organizer
      日本心臓血管麻酔学会第25回学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 悪性高熱症患者で発見された1型リアノジン受容体の一塩基多型E3756Qの機能解析2019

    • Author(s)
      近藤隆志,安田季道,神﨑理英子,向田圭子,濱田宏,河本昌志
    • Organizer
      日本麻酔科学会第66回学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 高度肺高血圧症を伴う心室中隔欠損症および大動脈弁狭窄症に対する根治手術の術後管理に高流量鼻カニュラによる一酸化窒素吸入療法が有用であった1例2019

    • Author(s)
      近藤 隆志, 三好 寛二, 濱田宏
    • Organizer
      日本心臓血管麻酔学会第24回学術大会
    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi