Novel multiplex immunohistochemical analysis for tumor heterogeneity and tumor immune microenvironment in head and neck cancer
| Project/Area Number |
19K18814
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 56050:Otorhinolaryngology-related
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
Tsujikawa Takahiro 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80713916)
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 頭頸部癌 / 腫瘍免疫 / イメージング / 癌微小環境 / バイオマーカー |
|---|
| Outline of Research at the Start |
頭頸部癌をはじめ、様々な癌種で癌細胞の腫瘍内不均一性と腫瘍組織内に存在する免疫細胞の組成や性質が癌の進展や治療抵抗性と関わることが知られてきた。本研究では、12種類のエピトープを定量的に解析可能な多重免疫染色・イメージサイトメトリー法を用いて、免疫特性に加え、癌の悪性形質に関するCancer Hallmarkパネルを作成の上、頭頸部扁平上皮癌組織を解析し、免疫細胞と癌の悪性形質の両者を統合的に解析し、両者の相互作用、位置分布や不均一性、臨床病理学的意義を検証する。これにより、免疫療法をふくむ新規・既存治療の最適化につながる、単独ないしは複合的な組織バイオマーカーの同定を目的とする。
|
| Outline of Final Research Achievements |
Based on multiplex immunohistochemistry and image cytometry, enabling immunohistochemical analysis of 12 epitopes from a single formalin-fixed paraffin-embedded section, the intratumoral heterogeneity and immune-based characteristics of head and neck cancer have been investigated. Immunohistochemical markers capable of assessing various malignant capabilities of cancer cells were extracted from the past 10 years of publications using systematic review and meta-analysis, and a panel was constructed to examine 10 representative malignant capabilities of head and neck cancer in a single section. A technical basis that enables integrated analysis of both cancer cells and immune cells has been completed, and is expected to contribute to the development of prognostic and predictive biomarkers in the future.
|
| Academic Significance and Societal Importance of the Research Achievements |
癌治療の効果予測や治療抵抗性制御において腫瘍内の癌細胞や免疫特性の不均一性の定量的評価が課題となっている。本研究課題で、低コストの多重染色技術を用いて、従来の方法では実現困難であった同一組織上での癌細胞と免疫細胞の特性評価法が確立されたことから、今後の頭頸部癌への治療効果予測、最適化につながり、治療成績の向上、副作用・医療費低減への寄与が期待される。
|
Report
(4 results)
Research Products
(49 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Evaluation of Cyclophosphamide/GVAX Pancreas Followed by Listeria-Mesothelin (CRS-207) with or without Nivolumab in Patients with Pancreatic Cancer2020
Author(s)
41)Tsujikawa T, Crocenzi T, Durham JN, Sugar EA, Wu AA, Onners B, Nauroth JM, Anders RA, Fertig EJ, Laheru DA, Reiss K, Vonderheide RH, Ko AH, Tempero MA, Fisher GA, Considine M, Danilova L, Brockstedt DG, Coussens LM, Jaffee EM, Le DT.
-
Journal Title
Clinical Cancer Research
Volume: 26
Issue: 14
Pages: 3578-3588
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
[Journal Article] 免疫的癌微小環境解析を通じた頭頸部癌薬物療法の治療効果予測2020
Author(s)
辻川敬裕, 吉村佳奈子, 光田順一, 佐分利純代, 竹中まり, 大村 学, 新井啓仁, 荻 寛志, 宮川 文, 小西英一, 伊東恭子, 平野 滋.
-
Journal Title
頭頸部癌
Volume: 45(4)
Pages: 362-365
NAID
Related Report
-
-
-
-
[Journal Article] Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer2019
Author(s)
Reddy SM, Reuben A, Barua S, Jiang H, Zhang S, Wang L, Gopalakrishnan V, Hudgens CW, Tetzlaff MT, Reuben JM, Tsujikawa T, Coussens LM, Wani K, He Y, Villarreal L, Wood AL, Rao A, Woodward WA, Ueno NT, Krishnamurthy S, Wargo, JA, Mittendorf EA
-
Journal Title
Cancer Immunology Research
Volume: 7
Issue: 6
Pages: 1025-1035
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
[Presentation] Longitudinal profiling of intratumoral heterogeneity of head and neck squamous cell carcinoma via 14-biomarker multiplex immunohistochemistry2021
Author(s)
Mitsuda J, Tsujikawa T, Yoshimura K, Saburi S, Kimura A, Morimoto H, Ohmura G, Arai A, Ogi H, Shibata S, Miyagawa-Hayashino A, Konishi E, Itoh K, Hirano S
Organizer
JCA-AACR Precision Cancer Medicine International Conference 2021
Related Report
Int'l Joint Research
-
[Presentation] Intratumoral PD-1+ helper T cells are associated with poor prognosis in head and neck squamous cell carcinoma2021
Author(s)
Yoshimura K, Tsujikawa T, Mitsuda J, Saburi S, Kimura A, Morimoto H, Ohmura G, Arai A, Ogi H, Shibata S, Thibault G, Chang YH, Clayburgh DR, Yasukawa s, Miyagawa-Hayashino A, Konishi E, Itoh K, Hirano S
Organizer
JCA-AACR Precision Cancer Medicine International Conference 2021
Related Report
Int'l Joint Research
-
[Presentation] Spatially-resolved immune microenvironmental profiles of follicular thyroid carcinoma2021
Author(s)
Saburi S, Tsujikawa T, Miyagawa-Hayashino A, Mitsuda J, Yoshimura K, Kimura A, Morimoto H, Ohmura G, Arai A, Ogi H, Konishi E, Itoh K, Sugino K, Hirano S
Organizer
JCA-AACR Precision Cancer Medicine International Conference 2021
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
[Presentation] 化学放射線治療後の局所再発と相関する頭頸部癌免疫的微小環境特性の解析2020
Author(s)
辻川敬裕, Grace Banik, 光田順一, 吉村佳奈子, 佐分利純代, 竹中まり, 大村 学, 新井啓仁, Daniel R. Clayburgh, Lisa M. Coussens, 平野 滋
Organizer
第30回日本頭頸部外科学会
Related Report
-
-
-
-
-
-
-
[Presentation] Symposia Tumor heterogeneity at single-cell resolution. 29-biomarker multiplex IHC reveals tumor immune microenvironment profiles associated with recurrence of HNSCC2019
Author(s)
Tsujikawa T, Banik G, Betts CB, Liudahl S, Sivagnanam S, Kawashima R, Cotechini T, Larson W, Mitsuda J, Saburi S, Clayburgh DR, Hirano S, Coussens LM
Organizer
第78回日本癌学会学術総会
Related Report
Invited
-
-
-
-
-
-
