• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Hard tissue regeneration using transplantation of adipose tissue-derived regenerative cells

Research Project

Project/Area Number 19K19244
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionShowa University

Principal Investigator

Masakatsu Itose  昭和大学, 歯学部, 助教 (30806576)

Project Period (FY) 2019-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords脂肪組織由来再生細胞 / 軟骨組織 / ADRCs / 肪組織由来再生細胞(ADRCs) / 硬組織再生 / ナノインデンテーション / 脂肪組織由来再生細胞(ADRCs)
Outline of Research at the Start

本研究の概要は、外科的手術や歯周疾患などで生じる顎骨欠損や、顎関節損傷による骨・軟骨欠損は、機能・審美障害によってQOL低下に影響するため、組織欠損の回復・改善は、口腔外科臨床の重要課題のひとつであり、脂肪組織由来再生細胞(ADRCs)を、歯科領域の硬組織再生療法へ応用することである。ADRCsは多分化能をもつなど幹細胞の特性を備えるほか、採取時の簡便性・侵襲の低さから、形成外科領域などにおいて再生医療の細胞ソースとして応用されており、本研究課題での頬部脂肪体などの脂肪組織中のADRCsを応用した顎骨欠損の再建、歯周疾患、顎関節損傷の改善に向けた新たな再生技術のとして有用である。

Outline of Final Research Achievements

Adipose tissue-derived regenerated cells (ADRCs) were investigated for the aim of improving bone and cartilage defects. ADRCs had pluripotent capacity of differentiating into osteoblast-like cells and chondrocyte-like cells. Assuming a model of articular disc or temporomandibular joint injury, an atelocollagen sponge infused with ADRCs was transplanted into a rat knee joint in which the medial meniscus was partially resected. Compared with the sponge alone, the atelocollagen sponge with ADRCs improved the repair of cartilage-like tissue. In dynamic mechanical analysis, higher elastic moduli and lower fluid permeability of regenerated meniscal tissue demonstrated a favorable structure-function relationship required for native menisci. ADRCs will be useful in future clinical cell-based therapy strategies, including as a cell source for reconstruction of hard tissue.

Academic Significance and Societal Importance of the Research Achievements

脂肪組織から単離したADRCsは、骨芽細胞様細胞や軟骨細胞様細胞へと分化できる多分化能を有し、軟骨修復に大きく寄与した。骨髄などに比べ低侵襲かつ移植時に新鮮な細胞が十分量採取できるADRCsは、硬組織再生医療の細胞ソースとして有用と考える。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (5 results)

All 2020

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results)

  • [Journal Article] Prospects of neural crest-derived cells from oral and dentofacial tissues for application in regenerative medicine2020

    • Author(s)
      Tetsuo Suzawa, Hiroshi Yoshida
    • Journal Title

      Oral Science International

      Volume: 17 Issue: 3 Pages: 115-125

    • DOI

      10.1002/osi2.1064

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 急性期病院における周術期口腔機能管理の現状と有用性 -術後合併症に対する影響とリスク評価-2020

    • Author(s)
      葭葉清香、伏居玲香、糸瀬昌克、八十篤聡、代田達夫
    • Journal Title

      日本口腔科学会雑誌

      Volume: 69 Pages: 22-28

    • NAID

      130008068571

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 昭和大学横浜市北部病院 歯科・歯科口腔外科 開設後 8 年間における患者の臨床統計学的観察2020

    • Author(s)
      葭葉清香、渡辺仁資、伏居玲香、糸瀬昌克、長崎理佳、八十篤聡、代田達夫
    • Journal Title

      昭和学士会雑誌

      Volume: 79 Pages: 757-764

    • NAID

      130007841706

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Possible involvement of elastase in enhanced osteoclast differentiation by neutrophils through degradation of osteoprotegerin.2020

    • Author(s)
      Sugisaki R, Miyamoto Y, Yoshimura K, Sasa K, Kaneko K, Tanaka M, Itose M, Inoue S, Baba K, Shirota T, Chikazu D, Kamijo R
    • Journal Title

      Bone

      Volume: 132 Pages: 115216-115216

    • DOI

      10.1016/j.bone.2019.115216

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 昭和大学横浜市北部病院における周術期口腔機能管理の現状とその効果について2020

    • Author(s)
      葭葉 清香、糸瀬 昌克、伏居 玲香、代田 達夫
    • Organizer
      54回 NPO法人日本口腔科学会関東地方部会
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi