Assessment of brown adipose tissue density in children and pregnant women and investigation of factors affecting brown adipose tissue

• Project/Area Number: 19K20123
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Tokyo Medical University
• Principal Investigator: Miyuki Kuroiwa 東京医科大学, 医学部, 兼任助教 (40710091)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 褐色脂肪組織 / こども / 女性 / 妊婦 / 授乳婦 / 乳幼児 / 児童 / 近赤外時間分解分光法 / 褐色脂肪 / 褥婦 / 肥満予防 / 生活習慣病予防 / 生活習慣病 / 肥満 / 褐色脂肪細胞 / 生活習慣

Outline of Research at the Start

褐色脂肪組織はエネルギー消費の亢進および糖代謝やインスリン感受性の改善に効果がある事が発見され、肥満や生活習慣病予防の指標として注目されている。しかし、通常褐色脂肪の測定はFDG－PET/CTが用いられており、こども、妊婦、褥婦には侵襲性が強く不向きであったが、近年開発された近赤外時間分解分光法を用いる事により、どのような年齢・条件においても非侵襲的に、簡便に、多くのデータ採取が可能になった。そこで、我々は乳幼児、児童、妊婦、褥婦の褐色脂肪組織に影響を及ぼす因子の解明および遺伝的因子、環境因子などを網羅的に評価する事で、肥満および生活習慣病予防の指標作成を目的として本研究を進める。

Outline of Final Research Achievements

Brown adipose tissue has been found to increase energy expenditure and improve glucose metabolism and insulin sensitivity and has attracted attention as an indicator for obesity and lifestyle-related disease prevention. However, brown adipose tissue activity is usually measured using FDG-PET/CT, which is highly invasive and unsuitable for infants, children, pregnant women, and mothers. The use of near-infrared time-resolved spectroscopy has made it possible to measure brown adipose tissue density non-invasively and easily at any age and condition. In this study, we (1) evaluated brown adipose tissue density in infants and children, pregnant women and postpartum mothers, which had been difficult to measure and (2) investigated factors related to lifestyle and humoral factors that affect brown adipose tissue density.

Academic Significance and Societal Importance of the Research Achievements

本研究で用いた近赤外時間分解分光法は、侵襲なく褐色脂肪組織を測定することができ、未成年者や妊・褥婦も測定できる。未成年者を対象にすることで、成長や加齢に伴う褐色脂肪組織の変化を評価でき、またそれに関連する因子の同定が可能となる。これは小児肥満の予防だけでなく、その後の中高年肥満、代謝性疾患の予防に応用できる可能性がある。また、妊娠・産褥期の女性を測定した研究も存在しない。非妊時糖代謝が正常な女性でも、1割以上の女性が妊娠糖尿病になる可能性がある。これには糖代謝やインスリン感受性に関連のある褐色脂肪組織が関連していることも考えられ、本研究は妊娠糖尿病予防策の検討にも貢献できる可能性がある。

Research Products

• [Journal Article] The effects of 10-week strength training in the winter on brown-like adipose tissue vascular density (2022)
  • Author(s): Tanaka R, Hamaoka-Fuse S, Kuroiwa M, Kurosawa Y, Endo T, Kime R, Yoneshiro T, Hamaoka T
  • Journal Title: Int J Environ Res Public Health Volume: 19 Issue: 16 Pages: 10375-10375
  • DOI: 10.3390/ijerph191610375
  • Peer Reviewed / Open Access
• [Journal Article] Correlation of Plasma Amino Acid and Anthropometric Profiles with Brown Adipose Tissue Density in Humans (2021)
  • Author(s): Kuroiwa Miyuki、Hamaoka-Fuse Sayuri、Sugimoto Masahiro、Kurosawa Yuko、Aita Yasuko、Tomita Atsumi、Anjo Mikiko、Tanaka Riki、Endo Tasuki、Kime Ryotaro、Hamaoka Takafumi
  • Journal Title: Journal of Clinical Medicine Volume: 10 Issue: 11 Pages: 2339-2339
  • DOI: 10.3390/jcm10112339
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of brown adipose tissue vascular density on body adiposity in healthy Japanese infants and children (2021)
  • Author(s): Kuroiwa Miyuki、Hamaoka‐Fuse Sayuri、Amagasa Shiho、Kime Ryotaro、Endo Tasuki、Tanaka Riki、Kurosawa Yuko、Hamaoka Takafumi
  • Journal Title: Obesity Science & Practice Volume: 8 Issue: 2 Pages: 190-198
  • DOI: 10.1002/osp4.559
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 医療機関における外国人対応困難事例のテキストマイニング分析による探索的検討 (2021)
  • Author(s): 久米絢弓 黒岩美幸 久米美代子
  • Journal Title: 日本ウーマンズヘルス学会誌 Volume: 19-2 Pages: 13-21
  • Peer Reviewed
• [Journal Article] 福島原子力発電所事故後に自主避難した0歳児の母親の体験 (2021)
  • Author(s): 久米美代子 後藤恭一 黒岩美幸 久米絢弓
  • Journal Title: 日本ウーマンズヘルス学会誌 Volume: 19-2 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Vigorous-Intensity Physical Activities Are Associated with High Brown Adipose Tissue Density in Humans (2020)
  • Author(s): Tanaka Riki、Fuse Sayuri、Kuroiwa Miyuki、Amagasa Shiho、Endo Tasuki、Ando Akira、Kime Ryotaro、Kurosawa Yuko、Hamaoka Takafumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 17 Issue: 8 Pages: 2796-2796
  • DOI: 10.3390/ijerph17082796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Near-Infrared Time-Resolved Spectroscopy for Assessing Brown Adipose Tissue Density in Humans: A Review (2020)
  • Author(s): Hamaoka Takafumi、Nirengi Shinsuke、Fuse Sayuri、Amagasa Shiho、Kime Ryotaro、Kuroiwa Miyuki、Endo Tasuki、Sakane Naoki、Matsushita Mami、Saito Masayuki、Yoneshiro Takeshi、Kurosawa Yuko
  • Journal Title: Frontiers in Endocrinology Volume: 11 Pages: 261-261
  • DOI: 10.3389/fendo.2020.00261
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of Capsinoid Intake on Brown Adipose Tissue Vascular Density and Resting Energy Expenditure in Healthy, Middle-Aged Adults: A Randomized, Double-Blind, Placebo-Controlled Study (2020)
  • Author(s): Fuse Sayuri、Endo Tasuki、Tanaka Riki、Kuroiwa Miyuki、Ando Akira、Kume Ayami、Yamamoto Akiko、Kuribayashi Kanna、Somekawa Shinji、Takeshita Masamichi、Hashimoto Masaki、Kime Ryotaro、Kurosawa Yuko、Hamaoka Takafumi
  • Journal Title: Nutrients Volume: 12 Issue: 9 Pages: 2676-2676
  • DOI: 10.3390/nu12092676
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 筋収縮時のエネルギー代謝 (2020)
  • Author(s): 浜岡隆文 布施沙由理 遠藤祐輝 黒岩美幸 黒澤裕子 木目良太郎
  • Journal Title: ディサースリア臨床研究 Volume: 10-1 Pages: 20-26
• [Presentation] 近赤外時間分解分光法で測定したこどもと妊婦の褐色脂肪について (2022)
  • Author(s): 黒岩美幸、布施沙由理、田中璃己、遠藤祐輝、内藤玉緒、木目良太郎、黒澤裕子、浜岡隆文
  • Organizer: 第28回医用近赤外線分光法研究会
  • Invited
• [Presentation] Relationship between brown adipose tissue density in healthy children and abnormalities during pregnancy and nutrition status during infancy (2022)
  • Author(s): Kuroiwa Miyuki、Fuse-Hamaoka Sayuri、Tanaka Riki、Endo Tasuki、Tamao Naito、Kime Ryotaro、Yuko Kurosawa、Hamaoka Takafumi
  • Organizer: International Congress on obesity 2022
  • Int'l Joint Research
• [Presentation] 妊婦、授乳婦、非妊時女性の褐色脂肪組織密度の違い (2022)
  • Author(s): 黒岩美幸、布施沙由理、田中璃己、遠藤祐輝、内藤玉緒、木目良太郎、黒澤裕子、浜岡隆文
  • Organizer: 第43回日本肥満学会
• [Presentation] Seasonal differences in brown adipose tissue vascular density determined using total hemoglobin concentration in the supraclavicular region among Japanese healthy adults (2022)
  • Author(s): Sayuri Fuse, Miyuki Kuroiwa, Riki Tanaka, Tasuki Endo, Ryotaro Kime, Yuko Kurosawa, Takafumi Hamaoka
  • Organizer: Photonics West BiOS
  • Int'l Joint Research
• [Presentation] Brown adipose tissue density negatively correlates changes in isoleucine concentration during 10week strength training (2022)
  • Author(s): Riki Tanaka, Sayuri Fuse, Miyuki Kuroiwa, Masahiro Sugimoto, Yuko Kurosawa, Yasuko Aita, Atsumi Tomita, Mikiko Anjo, Tasuki Endo, Ryotaro Kime, Takafumi Hamaoka
  • Organizer: 第188回東京医科大学医学会総会
• [Presentation] Brown adipose tissue density inversely correlates changes in plasma branched-chain amino acids elicited by strength training (2022)
  • Author(s): Riki Tanaka, Sayuri Fuse, Miyuki Kuroiwa, Masahiro Sugimoto, Yuko Kurosawa, Yasuko Aita, Atsumi Tomita, Mikiko Anjo, Tasuki Endo, Ryotaro Kime, Takafumi Hamaoka
  • Organizer: Photonics West BiOS
  • Int'l Joint Research
• [Presentation] Total hemoglobin concentrations measured in supraclavicular region in children and adults using near-infrared time-resolved spectroscopy Presentation (2022)
  • Author(s): Miyuki Kuroiwa, Sayuri Fuse, Riki Tanaka, Tasuki Endo, Ryotaro Kime, Yuko Kurosawa, Takafumi Hamaoka
  • Organizer: Photonics West BiOS
  • Int'l Joint Research
• [Presentation] Correlation of plasma amino acid and anthropometric profiles with brown adipose tissue density in humans (2021)
  • Author(s): Miyuki Kuroiwa, Sayuri Hamaoka-Fuse, Masahiro Sugimoto, Yuko Kurosawa, Yasuko Aita, Atsumi To-mita, Mikiko Anjo, Riki Tanaka, Tasuki Endo, Ryotaro Kime, and Takafumi Hamaoka
  • Organizer: ObesityWeek 2021.
  • Int'l Joint Research
• [Presentation] 持久性鍛錬者と非鍛錬者における一定負荷運動時の活動筋組織酸素動態の差異 (2021)
  • Author(s): 遠藤祐輝 木目良太郎 布施沙由理 田中璃己 黒岩美幸 黒澤裕子 浜岡隆文
  • Organizer: 第76回日本体力医学会
• [Presentation] Relationship between brown adipose tissue density and plasma amino acids (2021)
  • Author(s): Atsumi Tomita, Yasuko Aita, Miyuki Kuroiwa, Sayuri Hamaoka-Fuse, Yuko Kurosawa, Mikiko Anjo, Riki Tanaka, Tasuki Endo, Ryotaro Kime, Masahiro Sugimoto, Takafumi Hamaoka
  • Organizer: Metabolomics 2021 （Online）
  • Int'l Joint Research
• [Presentation] ヒト褐色脂肪組織に与える環境要因の検索 (2021)
  • Author(s): 布施沙由理，二連木晋輔，黒岩美幸，遠藤祐輝，田中璃己，木目良太郎，黒澤裕子，浜岡隆文
  • Organizer: 第40回日本臨床運動療法学会学術集会（シンポジスト）
  • Invited
• [Presentation] 近赤外時間分解分光法を用いて評価したヒト褐色脂肪組織密度と関連する因子の検討 (2021)
  • Author(s): 布施沙由理，黒岩美幸，遠藤祐輝，田中璃己，木目良太郎，黒澤裕子，浜岡隆文
  • Organizer: 第27回医用近赤外線分光法研究会・第25回酸素ダイナミクス研究会合同研究会(シンポジスト)
  • Invited
• [Presentation] Greater muscle oxygenation during moderate-intensity constant-work exercise in endurance athletes (2021)
  • Author(s): Tasuki Endo, Rotaro Kime, Riki Tanaka, Sayuri Fuse, Miyuki Kuroiwa, Yuko Kurosawa, Takafumi Hamaoka
  • Organizer: SPIE Photonics West 2022
  • Int'l Joint Research
• [Presentation] 運動トレーニングによる褐色脂肪組織密度と血中BCAA濃度の関連 (2021)
  • Author(s): 田中璃己，布施沙由理，黒岩美幸，杉本昌弘，黒澤裕子，相田泰子，富田淳美，安生幹子，遠藤祐輝，木目良太郎，浜岡隆文
  • Organizer: 第27回医用近赤外線分光法研究会
• [Presentation] Brown Adipose Tissue Density, Determined by Total Hemoglobin Concentration in the Supraclavicular Region, is Associated with Body Adiposity in children (2021)
  • Author(s): Miyuki Kuroiwa, Sayuri Fuse, Shiho Amagasa, Riki Tanaka, Tasuki Endo, Ryotaro Kime, Yuko Kurosawa, Takafumi Hamaoka
  • Organizer: SPIE Photonics West
  • Int'l Joint Research
• [Presentation] Effect of the season of conception on brown adipose tissue density, determined by total hemoglobin concentration in the supraclavicular region, and body adiposity (2021)
  • Author(s): Sayuri Fuse, Yuko Kurosawa, Miyuki Kuroiwa, Riki Tanaka, Tasuki Endo, Ryotaro Kime, Takafumi Hamaoka
  • Organizer: SPIE Photonics West
  • Int'l Joint Research
• [Presentation] 乳幼児の褐色脂肪組織とカウプ指数との関連 (2021)
  • Author(s): 黒岩美幸 布施沙由理 黒澤裕子 田中璃己 天笠志保 遠藤祐輝 安藤啓 久米絢弓 木目良太郎 浜岡隆文
  • Organizer: 第41回日本肥満学会
• [Presentation] ジヒドロカプシエイトの継続摂取が褐色脂肪組織増強に与える機能性の検証 (2021)
  • Author(s): 布施沙由理 田中璃己 遠藤祐輝 黒岩美幸 木目良太郎 黒澤裕子 山本晃子 栗 林かんな 浜岡隆文
  • Organizer: 第41回日本肥満学会
• [Presentation] Relationship between an index of brown adipose tissue density in the supraclavicular region and leg muscle strength (2021)
  • Author(s): Riki Tanaka, Sayuri Fuse, Miyuki Kuroiwa, Tasuki Endo, Ryotaro Kime, Yuko Kurosawa, Takafumi Hamaoka
  • Organizer: SPIE Photonics West
  • Int'l Joint Research
• [Presentation] 鎖骨上窩の褐色脂肪組織密度指標と下肢筋力は関連する (2020)
  • Author(s): 田中璃己 布施沙由理 黒岩美幸 遠藤祐輝 木目良太郎 黒澤裕子 浜岡隆文
  • Organizer: 第75回体力医学会
• [Presentation] Skeletal muscle and brown adipose tissue characteristics determined by near-infrared time-resolved spectroscopy in Human (2020)
  • Author(s): Takafumi Hamaoka, Sayuri Fuse, Shiho Amagasa, Ryotaro Kime, Miyuki Kuroiwa, Tasuki Endo, Riki Tanaka, Yuko Kurosawa
  • Organizer: CME2020
  • Int'l Joint Research
• [Presentation] 中強度自転車運動中における筋組織血液量の増加が散乱係数変化 に及ぼす影響 (2020)
  • Author(s): 遠藤祐輝 木目良太郎 庭山雅嗣 布施沙由理 田中璃己 黒岩美幸 村瀬訓生 黒澤裕子 浜岡隆文
  • Organizer: 第24回酸素ダイナミクス研究会
• [Presentation] 筋酸素消費量の測定法とその限界 (2020)
  • Author(s): 木目良太郎 遠藤祐輝 田中璃己 布施沙由理 黒岩美幸 黒澤裕子 浜岡隆文
  • Organizer: 第24回酸素ダイナミクス研究会