• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pioneer plant growing at an old mine site could provide a safe site for tree seedlings via enhancing infection of functional microbes

Research Project

Project/Area Number 19K20473
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionForest Research and Management Organization (2023)
Hokkaido University (2021-2022)
Japan Atomic Energy Agency (2019-2020)

Principal Investigator

Haruma Toshikatsu  国立研究開発法人森林研究・整備機構, 森林総合研究所, 任期付研究員 (40836417)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsススキ / アカマツ / 内生菌 / 重金属 / 鉱山跡地 / 鉱山 / 重金属耐性 / 重金属ストレス / 樹木実生
Outline of Research at the Start

現在、高濃度の重金属を含む鉱山跡地の緑化において、遷移初期草本の定着だけでなく森林形成を考慮した樹木実生の定着が望まれている。鉱山跡地の遷移初期草本であるススキは根の内生菌によって有害金属耐性が増強されることが報告されている。ススキの周囲の樹木実生が比較的健全に生育していることから、ススキの周囲は樹木実生の生育の場として機能する可能性を見出した。本研究では、鉱山跡地におけるススキの周囲の樹木実生と、単独で生育している樹木実生の代謝産物や内生菌の感染率などを比較することで、樹木実生の定着に対するススキおよびその内生菌の影響を考察する。

Outline of Final Research Achievements

At an ole mine site, Miscanthus sinensis and Pinus densiflora can grow naturally, showing that they are useful for revegetation. Although P. densiflora sprouts (seedlings) were growing healthily inside M. sinensis, the leaves of P. densiflora outside M. sinensis were brown, indicating that the growth of the plants had been inhibited. Because microorganisms growing in roots contribute to heavy metal stress tolerance in plants, microorganisms were isolated from roots of M. sinensis and P. densiflora seedlings. As the results, common microorganisms were isolated. In addition, measurement of soil temperature showed that daily soil temperature change (diurnal range) were smaller inside M. sinensis than outside. These results suggest that M. sinensis could promote establishment of P. densiflora seedlings in old mine sites by sharing microorganisms and reducing diurnal range in soil temperature.

Academic Significance and Societal Importance of the Research Achievements

日本の高度成長を支えた鉱山の多くは、現在操業が停止している。そのため、国内には多くの鉱山跡地があり、付近住民の安全の他に環境・景観などの観点から、その緑化が強く望まれている。従来ススキなどの草本はアカマツ等樹木の侵入・定着を妨げるとされてきた。しかし本研究は、鉱山跡地のような強い環境ストレス下では、ススキがアカマツ実生の生育を促進し、定着に寄与する可能性を示唆した。これらの結果は、特定の条件下では草本が樹木の定着を促進することを示しており、今後の鉱山や荒廃地などの緑化技術という社会的ニーズの高い技術に対して基礎的な知見を提供すると考えられた。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (28 results)

All 2024 2023 2022 2021 2019

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (21 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Book (1 results)

  • [Journal Article] Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress2024

    • Author(s)
      Haruma Toshikatsu、Doyama Kohei、Lu Xingyan、Noji Kenta、Masuya Hayato、Arima Takahiko、Tomiyama Shingo、Yamaji Keiko
    • Journal Title

      PLOS ONE

      Volume: 18 Issue: 5 Pages: 0286203-0286203

    • DOI

      10.1371/journal.pone.0286203

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phialocephala fortinii increases aluminum tolerance in Miscanthus sinensis growing in acidic mine soil2021

    • Author(s)
      T. Haruma, K. Yamaji, H. Masuya
    • Journal Title

      Letters in Applied Microbiology

      Volume: 73 Issue: 3 Pages: 300-307

    • DOI

      10.1111/lam.13514

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of Initial Growth and Respiration of Various Plants for Revegetation of Dumping Sites in Closed Mine2021

    • Author(s)
      黒澤陽子, 王莫非, 森茂太, 春間俊克, 野路建太, 土山紘平, 山路恵子, 富山眞吾
    • Journal Title

      Resources Processing

      Volume: 67 Issue: 3 Pages: 122-127

    • DOI

      10.4144/rpsj.67.122

    • NAID

      130008046006

    • ISSN
      1348-6012, 1349-9262
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metal Accumulation and Tolerance in Artemisia indica var. maximowiczii (Nakai) H. Hara. and Fallopia sachalinensis (F.Schmidt) Ronse Decr., a Naturally Growing Plant Species at Mine Site2021

    • Author(s)
      Lu Xingyan、Yamaji Keiko、Haruma Toshikatsu、Yachi Mitsuki、Doyama Kohei、Tomiyama Shingo
    • Journal Title

      Minerals

      Volume: 11 Issue: 8 Pages: 806-806

    • DOI

      10.3390/min11080806

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zn tolerance in the evergreen shrub, Aucuba japonica, naturally growing at a mine site: Cell wall immobilization, aucubin production, and Zn adsorption on fungal mycelia2021

    • Author(s)
      Doyama Kohei、Yamaji Keiko、Haruma Toshikatsu、Ishida Atsushi、Mori Shigeta、Kurosawa Yoko
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 9 Pages: 1-5

    • DOI

      10.1371/journal.pone.0257690

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fe, Mn and 238U Accumulations in Phragmites australis Naturally Growing at the Mill Tailings Pond; Iron Plaque Formation Possibly Related to Root-Endophytic Bacteria Producing Siderophores2021

    • Author(s)
      Nakamoto Yukihiro、Doyama Kohei、Haruma Toshikatsu、Lu Xingyan、Tanaka Kazuya、Kozai Naofumi、Fukuyama Kenjin、Fukushima Shigeru、Ohara Yoshiyuki、Yamaji Keiko
    • Journal Title

      Minerals

      Volume: 11 Issue: 12 Pages: 1337-1337

    • DOI

      10.3390/min11121337

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 鉱山跡地に自生するアオキの根面微生物と内生菌の機能2024

    • Author(s)
      土山紘平、山路恵子、春間俊克
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 鉱山集積場において、内生菌が関与したススキのストレス耐性機構の解明2024

    • Author(s)
      松代雄太、山路恵子、盧星燕、土山紘平、春間俊克、谷内美月、升屋勇人、富山眞吾
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 鉱山跡地に自生するススキによるアカマツ実生の定着促進機構の解明2024

    • Author(s)
      春間俊克、山路恵子、土山紘平、盧星燕、野路建太、升屋勇人、 有馬孝彦、富山眞吾
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 鉱山跡地の緑化修復後における植生遷移について2023

    • Author(s)
      山路恵子、土山紘平、春間俊克、盧星燕、谷内美月、松代雄太、黒澤陽子、森茂太、山縣三郎、富山眞吾
    • Organizer
      グリーンレメディエーション研究会シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] Heavy metal tolerance mechanisms in Abies sachalinensis growing at an old mine site.2023

    • Author(s)
      Toshikatsu Haruma, Yosuke Yamamoto, Naoto Nishimoto, Shingo Tomiyama.
    • Organizer
      The 70th Annual Meeting of the Ecological Society of Japan, 2023, March.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑化研究の最前線~鉱山跡地をいかに緑化するか~2023

    • Author(s)
      春間俊克、西本直人、山本耀介、富山眞吾
    • Organizer
      グリーンレメディエーション研究会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 鉱山跡地に自生する植物の重金属ストレス耐性機構の解明2023

    • Author(s)
      春間俊克
    • Organizer
      土質基礎研究委員会環境地 盤分科会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] The Effect of Root-endophytic Aquapteridospora sp. on Metal-tolerance of Miscanthus sinensis, Naturally Growing at a Storage Site.2022

    • Author(s)
      Yuta Matsushiro, Keiko Yamaji, Xingyan Lu, Kohei Doyama, Toshikatsu Haruma, Hayato Masuya, Shingo Tomiyama.
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functions of Metabolites Produced by the Root Endophyte, Pezicula ericae, infecting Aucuba japonica, naturally growing at a mine site.2022

    • Author(s)
      Kohei Doyama, Keiko Yamaji, Toshikatsu Haruma, Hayato Masuya, Shojiro Hishiyama, Atsushi Kato.
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Clarification of Heavy-metal Tolerance Mechanism in Polygonum thunbergii Growing Naturally in Mine Pond via Seasonal Field Analysis.2022

    • Author(s)
      Mitsuki Yachi, Kohei Doyama, Toshikatsu Haruma, Keiko Yamaji.
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elucidation of Heavy Metal Tolerance in Artemisia indica var. maximowiczii Growing at a Mine Site, Considering Root Endophytic Fungi.2022

    • Author(s)
      Xingyan Lu, Kohei Doyama, Toshikatsu Haruma, Shingo Tomiyama, Hayato Masuya, Keiko Yamaji.
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] A dark-septa endophyte might enhance survival rate of Pinus densiflora seedlings in Miscanthus sinensis patches at a storage site.2022

    • Author(s)
      Toshikatsu Haruma, Kenta Noji, Kohei Doyama, Lu Xingyan, Hayato Masuya, Saburo Yamagata, Shingo Tomiyama, Keiko Yamaji.
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヨシの根におけるウラン吸着能および鉄プラーク形成機構の解明2022

    • Author(s)
      春間俊克, 土山紘平, 田中万也, 高橋嘉夫, 福山賢仁, 小原義之, 山路恵子
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 鉱山跡地に生育するミゾソバの重金属蓄積能の経時的解析2022

    • Author(s)
      谷内美月, 土山紘平, 春間俊克, 山路恵子
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 鉱さいたい積場のヨシにおける重金属元素の蓄積及び鉄プラーク形成へ関与する内生細菌2022

    • Author(s)
      中本幸弘, 山路恵子, 春間俊克, 土山紘平, 盧星燕, 田中万也, 香西直文, 福山賢仁, 福嶋繁, 小原義之
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Elucidation of heavy metal tolerance in Artemisia indica var. maximowiczii growing at a mine site, considering root endophytic fungi interaction2022

    • Author(s)
      Xingyan LU, Kohei DOYAMA, Toshikatsu HARUMA, Shingo TOMIYAMA, Hayato MASUYA, Keiko YAMAJI
    • Organizer
      The 69th Annual Meeting of the Ecological Society of Japan
    • Related Report
      2021 Research-status Report
  • [Presentation] Functions of compound produced by the endophyte, Pezicula ericae, infecting Aucuba japonica roots2022

    • Author(s)
      Kouhei DOYAMA, Keiko YAMAJI, Toshikatsu HARUMA
    • Organizer
      The 69th Annual Meeting of the Ecological Society of Japan
    • Related Report
      2021 Research-status Report
  • [Presentation] 鉱山跡地に自生するアオキと内生菌Pezicula ericaeの機能2022

    • Author(s)
      土山紘平, 山路恵子, 春間俊克
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 鉱山跡地に自生するススキが関与したアカマツの有害金属耐性機構の解明2022

    • Author(s)
      春間俊克, 野路建太, 松代雄太, 土山紘平, 盧星燕, 升屋勇人, 富山眞吾, 山路恵子
    • Organizer
      環境資源工学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 鉱山集積場に自生するススキが関与したアカマツ実生の生残要因の解明2021

    • Author(s)
      春間俊克、山路恵子、野路建太、土山紘平、盧星燕、升屋勇人、黒澤陽子、森茂太
    • Organizer
      第68回日本生態学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 内生菌Phialocephala fortinii が増強するススキの Al 耐性機構2019

    • Author(s)
      春間俊克
    • Organizer
      第67回日本生体学会大会
    • Related Report
      2019 Research-status Report
  • [Book] 自生植物利用による緑化方法. In 休廃止鉱山の新たな緑化対策等に関するガイダンス2022

    • Author(s)
      山路恵子、春間俊克
    • Total Pages
      81
    • Publisher
      経済産業省
    • Related Report
      2021 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi