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Basic study of stiffness through mechanical and acoustic properties of brain tissue and molecular information

Research Project

Project/Area Number 19K20666
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Tamura Kazuki  浜松医科大学, 光尖端医学教育研究センター, 助教 (40822614)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords生体計測 / 超音波計測 / 質量イメージング / 定量的組織性状評価 / 超音波顕微鏡 / 弾性イメージング / 質量顕微鏡 / 質量スペクトル / 音響特性 / マルチモダリティ
Outline of Research at the Start

“硬さ”を指標とした臨床診療は古くから日常的に行われてきたが,その技術習得は難しい.また,超音波診断装置やMRIを用いた硬さ可視化技術の空間分解能は10 mm程度と低く,これよりも小さな病変の集合体としての特性を示しているため,細胞スケールでの理解を促進するためには新たな硬さ研究の方法論が必要となる.
本研究では,質量顕微鏡を使用してマウス脳の脳腫瘍部位を含む試料を対象に分子情報による詳細な病期ステージングを行い,超音波顕微鏡で収集される硬さ情報との関連性を検討する.

Outline of Final Research Achievements

This study investigated the correspondence between elasticity and molecular distribution in brain tumor tissues, aiming to understand pathological specimens using hardness as an index. Ultrasonic microscopy using broad frequency band focused ultrasound was used to measure elasticity. Two-dimensional images of acoustic impedance and speed of sound, which are related to volume elastic modulus, were used as indices of hardness. Molecular information were obtained two-dimensionally using mass microscopy. The spectral infomation acquired in each measured position identified the lipid species.The relationship between the hardness of the biological tissue and the molecules obtained from these two measurements confirmed that there were differences in the distribution of hardness and the distribution of lipids related to the tumor.

Academic Significance and Societal Importance of the Research Achievements

脂肪性肝疾患の肝臓を対象に組織に含まれる分子と硬さの関係について検討が行われた例が報告されている. 脂肪性肝疾患の特徴はびまん性の疾患である点であり試料全体で比較的均質である. 本研究ではラットの脳内の一部に局在する腫瘍を計測対象として,病理学的に腫瘍領域・周辺領域・非腫瘍領域の3つの領域の分子分布と硬さ分布を計測して比較した.分子分布では周辺領域の脂質近傍領域からも腫瘍に関係する脂質が検出されたが,硬さの面では周辺領域と非腫瘍部の違いは小さかった.以上の結果より組織の硬さの変化は脂質の蓄積だけでなく,細胞の構造的変化による変化の要因が強いことが示唆された.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Suppression of reflected signals from substrate as clutters for cell measurements using acoustic impedance microscopy2022

    • Author(s)
      Nagaoka Ryo、Hasegawa Hideyuki、Tamura Kazuki、Yoshida Sachiko、Hozumi Naohiro、Kobayashi Kazuto
    • Journal Title

      Ultrasonics

      Volume: 118 Pages: 106580-106580

    • DOI

      10.1016/j.ultras.2021.106580

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantifying scattering from dense media using two-dimensional impedance maps2020

    • Author(s)
      Tamura Kazuki、Mamou Jonathan、Yoshida Kenji、Yamaguchi Tadashi、Franceschini Emilie
    • Journal Title

      The Journal of the Acoustical Society of America

      Volume: 148 Issue: 3 Pages: 1681-1691

    • DOI

      10.1121/10.0001972

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ラット臓器の凍結試料における音速評価精度の検討2022

    • Author(s)
      瀬戸 駿, 伊能 舞, 野口 和馬, 田村 和輝, 平田 慎之介, 吉田 憲司, 山口 匡
    • Organizer
      日本音響学会2022年春季研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 安定した生体音速評価を可能とする薄切試料作製および計測プロトコル2021

    • Author(s)
      瀬戸 駿, 伊能 舞, 野口 和馬, 田村 和輝, 平田 慎之介, 吉田 憲司, 山口 匡
    • Organizer
      超音波研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Evaluation of relationship between liver structure and frequency dependency of speed of sound and attenuation2021

    • Author(s)
      Mai Ino, Kazuma Noguchi, Suguru Seto, Masaaki Omura, Kazuki Tamura, Shinnosuke Hirata, Kenji Yoshida, Tadashi Yamaguchi
    • Organizer
      The 42nd Symposium on UltraSonic Electronics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Examination of validity of sample preparation method for speed of sound evaluation in ultra-high frequency band2021

    • Author(s)
      Suguru Seto, Kazuma Noguchi, Kazuki Tamura, Shinnosuke Hirata, Kenji Yoshida, Tadashi Yamaguchi
    • Organizer
      The 42nd Symposium on UltraSonic Electronics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chemical fixation effects on speed of sound values obtained from a healthy rat using a 250-MHz acoustic microscopy2021

    • Author(s)
      Kazuki Tamura, Kazuyo Ito, Sachiko Yoshida, Jonathan Mamou, Katsutoshi Miura, Seiji Yamamoto
    • Organizer
      IEEE International Ultrasonic Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 試料作製法の違いが生体組織の音速評価に与える影響の検討2021

    • Author(s)
      瀬戸 駿, 野口 和馬, 田村 和輝, 平田 慎之介, 吉田 憲司, 山口 匡
    • Organizer
      日本音響学会2021年秋季研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ラット臓器の音速の周波数依存性に関する基礎検討2021

    • Author(s)
      伊能 舞, 野口 和馬, 瀬戸 駿, 大村 眞朗, 田村 和輝, 平田 慎之介, 吉田 憲司, 山口 匡
    • Organizer
      超音波研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 凍結切片法を用いた脳腫瘍組織の音速計測2021

    • Author(s)
      田村和輝, 伊藤一陽, 三浦克敏, 山本清二
    • Organizer
      日本超音波医学会 第94回学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Differences in acoustical property between normal and tumor cells in a rat brain tumor based on cell nuclei density2020

    • Author(s)
      Kazuki Tamura
    • Organizer
      The 41st Symposium on Ultrasonic Electronics
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Quantitative ultrasound fat detection using a weight filter and a double Nakagami distribution model2019

    • Author(s)
      Kazuki Tamura
    • Organizer
      The IEEE International Ultrasonics Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Novel method of lipid content quantification using double-Nakagami distribution model in rat liver steatosis2019

    • Author(s)
      Kazuki Tamura
    • Organizer
      The Symposium on UltraSonicElectronics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 構造因子を考慮した後方散乱波解析への音響特性分布の適用手法の検討2019

    • Author(s)
      田村和輝
    • Organizer
      バイオ超音波顕微鏡研究会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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