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Foraminiferal elemental composition and calcification process

Research Project

Project/Area Number 19K21197
Project/Area Number (Other) 18H06074 (2018)
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund (2019)
Single-year Grants (2018)
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionJapan Agency for Marine-Earth Science and Technology (2019)
National Museum of Nature and Science, Tokyo (2018)

Principal Investigator

NAGAI Yukiko  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(超先鋭技術開発プログラム), 技術スタッフ (20822612)

Project Period (FY) 2018-08-24 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords電子顕微鏡 / バイオミネラリゼーション / 有孔虫 / FIB(集束イオンビーム) / カルサイト / 微細構造観察 / FIB(集束イオンビーム)
Outline of Research at the Start

石灰質有孔虫の殻に含まれる微量元素や同位体比は、環境に依存性して変化する為、古環境間接指標として広く応用されている。しかしその組成は種間の違いが大きい。その違いをもたらす要因はまだ分かっていない為、有孔虫殻の古環境間接指標としての信頼性向上を阻んできた。Mgの過多は殻形成過程に関連すると推測されている。そこで本研究では有孔虫が殻形成の際に有機膜で海水から隔絶した空間を作り、その中で石灰化を行うことに着目し『有孔虫殻の元素組成のばらつきは、膜状構造の水密性の違い、すなわち外界との隔絶の度合いの違いに起因する』という仮説を検証する為に、種によって大きく異なるMgの含有率と膜状構造の形態を比較する。

Outline of Final Research Achievements

Trace element and isotopic compositions of calcareous foraminifers shells have been used as various paleo-environmental proxies, and their chemical/isotopic compositions are known to differ not only from environmental factors but also from species to species. This species-specific compositional variation has been called "vital effect". The cause of vital effect remains unclear, as do the formation process of foraminiferal shells and the uptake of elements with the cellular level. In the present project, we used three species of foraminifera with different Mg content in the shells. Furthermore, shell formation steps and the completeness of the organic membrane covering the site of calcification have been observed with these three species. Besides, the cytoplasmic ultra-structures of the site of calcification show different morphology in each species.

Academic Significance and Societal Importance of the Research Achievements

Mg含有率の異なる有孔虫3種の殻形成過程の観察や殻形成部位の微細構造観察を行ったところ、殻形成に要する時間や殻形成部位への細胞質のアプローチに違いが見えてきた。このことから各々の有孔虫に異なる殻形成戦略があることが示された。地球科学的問題に対し生物学的なアプローチを加えたことで今日まで棚上げにされてきた種特異的な殻組成の変異の要因を知る緒を得た。今後は海水から殻への分配係数を把握することで、これまでの研究の信頼性をより高め、欠落していた環境因子に対する指標の開発にも繋がるだろう。他種での観察を続け、また細胞レベルでの元素の取り込み把握の為に細胞内外の元素の可視化を行う必要があると考えられる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • Research Products

    (30 results)

All 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (21 results) (of which Int'l Joint Research: 12 results,  Invited: 1 results)

  • [Journal Article] Guanine crystals regulated by chitin-based honeycomb frameworks for tunable structural colors of sapphirinid copepod, Sapphirina nigromaculata2020

    • Author(s)
      T. Kimura, M. Takasaki, R. Hatai, Y. Nagai, K. Uematsu, Y. Oaki, M. Osada, H. Tsuda, T. Ishigure, T. Toyofuku, S. Shimode, H. Imai
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 2266-2266

    • DOI

      10.1038/s41598-020-59090-4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shell formation of foraminifera -marine unicellular calcifiers-2019

    • Author(s)
      Takashi Toyofuku, Yukiko Nagai
    • Journal Title

      Journal of the Japanese Association for Crystal Growth

      Volume: 46 Pages: 02-02

    • NAID

      130007744151

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Foraminiferal Ultrastructure: A perspective From Fluorescent and Fluorogenic Probes2019

    • Author(s)
      Frontalini F.、Losada M. T.、Toyofuku T.、Tyszka J.、Golen J.、Nooijer L.、Canonico B.、Cesarini E.、Nagai Y.、Bickmeyer U.、Ikuta T.、Tsubaki R.、Besteiro Rodriguez C.、Al‐Enezi E.、Papa S.、Coccioni R.、Bijma J.、Bernhard J. M.
    • Journal Title

      Journal of Geophysical Research: Biogeosciences

      Volume: 124 Issue: 9 Pages: 2823-2850

    • DOI

      10.1029/2019jg005113

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Direct Two-Dimensional Time Series Observation of pH Distribution around Dissolving Calcium Carbonate Crystals in Aqueous Solution2019

    • Author(s)
      Kawano Jun、Toyofuku Takashi、Nishimura Kaede、Ueda Akiyuki、Nagai Yukiko、Kawada Sachiko、Teng Henry、Nagai Takaya
    • Journal Title

      Crystal Growth & Design

      Volume: 19 Issue: 8 Pages: 4212-4217

    • DOI

      10.1021/acs.cgd.9b00045

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complex factors shape phenotypic variation in deep-sea limpets2019

    • Author(s)
      Chen Chong、Watanabe Hiromi Kayama、Nagai Yukiko、Toyofuku Takashi、Xu Ting、Sun Jin、Qiu Jian-Wen、Sasaki Takenori
    • Journal Title

      Biology Letters

      Volume: 15 Issue: 10 Pages: 20190504-20190504

    • DOI

      10.1098/rsbl.2019.0504

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Euxinic Conditions on Planktic Foraminifers: Culture Experiments and Implications for Past and Future Environments2019

    • Author(s)
      Kuroyanagi Azumi、Toyofuku Takashi、Nagai Yukiko、Kimoto Katsunori、Nishi Hiroshi、Takashima Reishi、Kawahata Hodaka
    • Journal Title

      Paleoceanography and Paleoclimatology

      Volume: 34 Issue: 1 Pages: 54-62

    • DOI

      10.1029/2018pa003539

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Weaving of biomineralization framework in rotaliid foraminifera: implications for paleoceanographic proxies2018

    • Author(s)
      Nagai Yukiko、Uematsu Katsuyuki、Chen Chong、Wani Ryoji、Tyszka Jaroslaw、Toyofuku Takashi
    • Journal Title

      Biogeosciences

      Volume: 15 Issue: 22 Pages: 6773-6789

    • DOI

      10.5194/bg-15-6773-2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reading the Fine Print: Ultra-Microstructures of Foraminiferal Calcification Revealed Using Focused Ion Beam Microscopy2018

    • Author(s)
      Nagai Yukiko、Uematsu Katsuyuki、Wani Ryoji、Toyofuku Takashi
    • Journal Title

      Frontiers in Marine Science

      Volume: 5 Pages: 1-15

    • DOI

      10.3389/fmars.2018.00067

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Decalcification and survival of benthic foraminifera under the combined impacts of varying pH and salinity2018

    • Author(s)
      Charrieau Laurie M.、Filipsson Helena L.、Nagai Yukiko、Kawada Sachiko、Ljung Karl、Kritzberg Emma、Toyofuku Takashi
    • Journal Title

      Marine Environmental Research

      Volume: 138 Pages: 36-45

    • DOI

      10.1016/j.marenvres.2018.03.015

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Estimation of the calcification rate of foraminifera -exceptionally fast extending of the shell-2019

    • Author(s)
      Nagai Y., Toyofuku T.
    • Organizer
      日本地球惑星科学連合大会 2019年大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Estimation of foraminiferal calcification process from live imaging and FIB-SEM approach2019

    • Author(s)
      Toyofuku T., Nagai Y.
    • Organizer
      日本地球惑星科学連合大会 2019年大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcript assembly and quantification identify candidate genes for foraminiferal calcification2019

    • Author(s)
      Ujiie Y., Ishitani Y., Ishii S., Nagai Y., Takagi Y., Ikuta T., Toyofuku T.
    • Organizer
      日本地球惑星科学連合大会 2019年大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The coral reef-dwelling Peneroplis brough to light: recalcification during culture experiment2019

    • Author(s)
      Charrieau L., Nagai Y., Fujita K., Multer D., McIntosh I., Toyofuku T.
    • Organizer
      The Micropalaeontological Socity Foram-Nanno 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光プローブによる炭酸塩鉱物の形成/溶解場の2次元可視化2019

    • Author(s)
      川野潤, 大澤康太朗, 豊福高志, 長井裕季子, 田中淳也, 永井隆哉
    • Organizer
      日本地球惑星科学連合大会 2019年大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超微細構造などから推測される底生有孔虫Ammoniaの殻形成機序2019

    • Author(s)
      豊福高志, 長井裕季子
    • Organizer
      第14回バイオミネラリゼーションワークショップ
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 蛍光プローブを用いた2次元可視化手法による結晶の溶解/成長メカニズムの検討2019

    • Author(s)
      川野潤, 矢崎誠, 豊福高志, 長井裕季子, Henry Teng, 永井隆哉
    • Organizer
      日本鉱物学会2019年年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Formation Process of Organic membrane within calcareous test and early calcium carbonate deposition of benthic foraminifera Ammonia .2018

    • Author(s)
      Yukiko Nagai, Takashi Toyofuku, Chong Chen, Katsuyuki Uematsu
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Proton pumping accompanies calcification in foraminifera2018

    • Author(s)
      Takashi Toyofuku, Miki Y. Matsuo, Lennart Jan de Nooijer, Yukiko Nagai, Tetsuro Ikuta
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Foraminiferal ultrastructure: a perspective from fluorescent and fluorogenic probes2018

    • Author(s)
      Fabrizio Frontalini, Maria Teresa Losada, Takashi Toyofuku, Jaroslaw Tyszka, Jan Golen, Lennart de Nooijer, Barbara Canonico, Erica Cesarini, Yukiko Nagai, Tetsuro Ikuta, Remi Tsubaki, Stefano Papa, Rodolfo Coccioni, Jelle Bijma and Joan M. Bernhard
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Combined impacts of ocean acidification and desalination: culture experiments on the coastal species Ammonia sp. and Elphidium crispum2018

    • Author(s)
      Laurie Charrieau, Helena Filipsson, Yukiko Nagai, Sachiko Kawada, Karl Ljung, Emma Kritzberg and Takashi Toyofuku
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Test density of Ammonia sp. under variable pH2018

    • Author(s)
      Takashi Toyofuku, Yukiko Nagai, Katsunori Kimoto, Osamu Sasaki
    • Organizer
      JpGU meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ultra-microstructures of foraminiferal calcification observed using focused ion beam microscopy2018

    • Author(s)
      Yukiko Nagai, Takashi Toyofuku
    • Organizer
      JpGU meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcript assembly and quantification by RNA-Seq identifies candidate genes for foraminiferal biomineralization of Ammonia beccarii2018

    • Author(s)
      Yurika Ujiie, Yoshiyuki Ishitani, Shunichi Ishii, Yukiko Nagai, Yoshihiro Takaki, Takashi Toyofuku
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phylogenomic approach to the early evolution of Foraminifera2018

    • Author(s)
      Yoshiyuki Ishitani, Yurika Ujiie, Euki Yazaki, Takashi Toyofuku, Yuji Inagaki
    • Organizer
      International Symposium on Foraminifera (FORAMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 例外的に伸長が早い有孔虫の石灰化速度の推定2018

    • Author(s)
      長井裕季子, 豊福高志
    • Organizer
      第13回バイオミネラリゼーションワークショップ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 石灰化有孔虫における殻形成プロセス研究の進捗-現生有孔虫観察が切り拓く新たな地平-2018

    • Author(s)
      豊福高志, 長井裕季子
    • Organizer
      第4回地球環境史学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 有孔虫の炭酸塩の殻形成に関する分子機構-トランスクリプトーム解析から-2018

    • Author(s)
      氏家由利香, 石谷佳之, 石井俊一, 長井裕季子, 高木義弘, 豊福高志, 生田哲郎
    • Organizer
      第13回バイオミネラリゼーションワークショップ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 浮遊性有孔虫の持つカルサイト針状突起の解析と類似体の合成2018

    • Author(s)
      泉田健太, 高崎美宏, 長井裕季子, 緒明佑哉, 豊福高志, 今井宏明
    • Organizer
      日本化学会秋季事業 第8回CSJ化学フェスタ2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光プローブを用いた可視化手法による炭酸塩鉱物の成長/溶解プロセスの観察2018

    • Author(s)
      川野潤, 上田暁之, 豊福高志, 長井裕季子, 田中淳也, 永井隆哉
    • Organizer
      JpGU 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光プローブを用いた2次元可視化手法による結晶溶解プロセス観察2018

    • Author(s)
      川野潤, 豊福高志, 長井裕季子, 田中淳也, 永井隆哉
    • Organizer
      第47回結晶成長国内会議
    • Related Report
      2018 Annual Research Report

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Published: 2018-08-27   Modified: 2024-03-26  

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