Why female has greater resistance for skeletal muscle damage than male?: contribution of mitochondria

• Project/Area Number: 19K21491
• Project/Area Number (Other): 18H06414 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0909:Sports sciences, physical education, health sciences, and related fields
• Research Institution: Hiroshima University (2019); The University of Electro-Communications (2018)
• Principal Investigator: Watanabe Daiki 広島大学, 総合科学研究科, 助教 (30823281)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨格筋 / エストロゲン / 伸張性収縮 / 細胞内遊離Ca2+ / 細胞内カルシウム / 卵巣摘出 / 活性酸素種 / カルシウムイオン

Outline of Research at the Start

激しい運動後に長期にわたって疲労し続けることは，周知の事実である．興味深いことに，この長期にわたる疲労は，男性よりも女性のほうが生じにくいことが報告されている．しかしながら，なぜそのような違いが生じるのかについては明らかとなっておらず，本研究はこの点を明確にすることを目的とする．本研究は，女性に対する運動処方だけでなく，疲労を軽減させるサプリメントの開発などに有益であろう．

Outline of Final Research Achievements

The purpose of this study was to investigate the mechanism underlying greater resistance for muscle damage in female than male. To this end, we examined the sex difference in muscle damage after repeated eccentric contractions (ECCs) and the difference between ovariectomy-treated (OVX), OVX with estrogen-treated and sham-operated female mice in muscle damage after ECCs. The results of this study suggest that 1) a superior mitochondrial adaptation in female alleviates muscle damage at least after ECCs, and 2) such mitochondrial adaptation is enhanced by the estrogen.

Academic Significance and Societal Importance of the Research Achievements

本研究では，1) 雌性の方が雄性よりもミトコンドリアの運動に伴うダメージの緩衝能力が高い点，2) その原因はエストロゲンにある点を初めて明らかにした．本研究の結果は，男性と女性の運動後の適応の違いを説明するための学術的基礎となるだけでなく，今後，性別の特性を考慮した筋疲労や筋損傷への対処法やトレーニング法を考案するためにも，必要な知見となると思われる．

Research Products

• [Journal Article] Sex differences in mitochondrial Ca2+ handling in mouse fast-twitch skeletal muscle in vivo (2020)
  • Author(s): Watanabe Daiki、Hatakeyama Koji、Ikegami Ryo、Eshima Hiroaki、Yagishita Kazuyoshi、Poole David C.、Kano Yutaka
  • Journal Title: Journal of Applied Physiology Volume: 128 Issue: 2 Pages: 241-251
  • DOI: 10.1152/japplphysiol.00230.2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle (2019)
  • Author(s): Yu Kitaoka, Daiki Watanabe, Yudai Nonaka, Kazuyoshi Yagishita, Yutaka Kano, Daisuke Hoshino
  • Journal Title: Physiological Reports Volume: 7 Issue: 19
  • DOI: 10.14814/phy2.14266
  • Peer Reviewed / Open Access
• [Presentation] Sex differences in prolonged low-frequency force depression after eccentric contractions (2019)
  • Author(s): Watanabe, D., Ikegami, R., Kano, Y.
  • Organizer: Gordon Research Conference Muscle: Excitation-Contraction Coupling
  • Int'l Joint Research
• [Presentation] Sex differences in mitochondria Ca2+ handling properties in mouse skeletal muscle (2019)
  • Author(s): Watanabe, D., Hatakeyama, K., Ikegami, R., Eshima, H., Kano, Y.
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies Congress
  • Int'l Joint Research
• [Presentation] Sex differences in prolonged low-frequency force depression after eccentric contractions (2019)
  • Author(s): Daiki Watanabe, Ryo Ikegami, Yutaka Kano
  • Organizer: Gordon Research Conference
  • Int'l Joint Research
• [Presentation] Sex difference in mitochondria Ca2+ handling properties in mouse skeletal muscle (2019)
  • Author(s): Daiki Watanabe, Hatakeyama Koji, Ikegami Ryo, Eshima Hiroaki, Yutaka Kano
  • Organizer: Federation of the Asian and Oceanian Physiological Societies Congress
  • Int'l Joint Research