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Genome Scan for Genes underlying Global Warming Adaptation Using Next-generation Sequencing: Application to Genetic Diversity Projection

Research Project

Project/Area Number 19K21996
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 22:Civil engineering and related fields
Research InstitutionEhime University

Principal Investigator

Watanabe Kozo  愛媛大学, 沿岸環境科学研究センター, 教授 (80634435)

Project Period (FY) 2019-06-28 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsゲノム / 適応遺伝子 / 次世代シークエンシング / 遺伝的多様性 / 河川 / 気候変動 / 次世代シーケンシング
Outline of Research at the Start

気候変動に伴う温暖化や異常気象は,流域内の河川底生動物の遺伝的多様性(同種個体間の遺伝的変異)を劣化させる可能性がある。本研究は,気候変動下の水温・流速等の流域環境の変化への適応進化を予測するモデルを開発する。膨大なゲノムから環境選択を受けている遺伝子を次世代シークエンシング解析を活用して迅速に検索し,これら環境選択性遺伝子と環境変数の関係を定量的に結びつける機械学習モデルを開発する。そして,このモデルを全球気候モデルとカップリングすることにより,気候変動下の遺伝子型や遺伝的多様性の空間分布を予測する。

Outline of Final Research Achievements

In this study, we constructed a method to describe adaptive changes population genetic structure associated with changes in environmental gradients within a watershed due to climate change. We developed an adaptive evolution model for seven riverine aquatic insect species by searching the genome for environmentally selective loci that are affected by environmental factors related to global warming (e.g., water temperature and precipitation) and predicting their allele frequencies from environmental variables. Then, we predicted the spatial distribution of environmental variables at the basin scale under climate change scenarios, and predicted the spatial distribution of allele frequencies and genetic diversity by gapping with the above adaptive evolution model. The results showed that habitat reduction does not necessarily degrade genetic diversity when adaptive evolution is considered.

Academic Significance and Societal Importance of the Research Achievements

従来の気候変動下の遺伝的多様性の予測は,中立進化(環境選択を受けない)を前提としているが,現実には,例えば低水温に適応した遺伝子型は温暖化が起きれば,水温が低い高標高・高緯度地域に避難して存続できる可能性がある。しかし,従来法では中立遺伝子座が解析されてきたため,環境勾配に沿った遺伝子の「避難現象」を再現できず,遺伝的多様性の低下を過剰に予測する可能性が懸念される。本研究は、ゲノム上の環境選択性遺伝子に着目して,気候変動下における環境勾配に沿った遺伝子型の移動(避難)も考慮した上で,遺伝的多様性をより正確に予測するモデルを開発した点で高い学術的意義がある。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (20 results)

All 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 9 results,  Open Access: 8 results) Presentation (10 results) (of which Int'l Joint Research: 10 results,  Invited: 3 results)

  • [Int'l Joint Research] カリフォルニア州立大学バークレー校(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Detailed Description and Illustration of Larva, Pupa, and Imago of Holorusia mikado (Westwood, 1876) (Diptera: Tipulidae) from Japan2021

    • Author(s)
      Kolcsar, L-P, D. T. Nakamura, Kato, and K. Watanabe
    • Journal Title

      Biodiversity Data Journal

      Volume: 9

    • DOI

      10.3897/bdj.9.e58009

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simulation Modeling Reveals the Evolutionary Role of Landscape Shape and Species Dispersal on Genetic Variation within a Metapopulation2020

    • Author(s)
      Chiu, M-C, K. Nukazawa, T. M. Carvajal, V. H. Resh, B. Li, and K. Watanabe
    • Journal Title

      Ecography

      Volume: 43 (12) Issue: 12 Pages: 1891-1901

    • DOI

      10.1111/ecog.05118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Branching Networks Can Have Opposing Influences on Genetic Variation in Riverine Metapopulations2020

    • Author(s)
      Chiu, M-C, B. Li, K. Nukazawa, V. H. Resh, T. M. Carvajal, and K. Watanabe
    • Journal Title

      Diversity and Distributions

      Volume: 26 (12) Issue: 12 Pages: 1813-1824

    • DOI

      10.1111/ddi.13160

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Revision of Japanese species of Nipponomyia Alexander, 1924 (Diptera, Pediciidae)2020

    • Author(s)
      Kolcsar, L. P., D. Kato, M. Gamboa, and K. Watanabe
    • Journal Title

      ZooKeys

      Volume: 1000 Pages: 71-105

    • DOI

      10.3897/zookeys.1000.55021

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synopsis of the genus Ulomorpha Osten Sacken, 1869 (Diptera, Limoniidae) in Japan2020

    • Author(s)
      Kato, D., K. Watanabe, and L-P Kolcs?r
    • Journal Title

      ZooKeys

      Volume: 999 Pages: 147-163

    • DOI

      10.3897/zookeys.999.52831

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Remarkable Anoxia Tolerance by Stoneflies from a Floodplain Aquifer2020

    • Author(s)
      Malison, R. L., B. K. Ellis, A. G. DelVecchia, H. N. Jacobson, B. K. Hand, G. Luikart, H. A. Woods, M. Gamboa, K. Watanabe, and J. A. Stanford
    • Journal Title

      Ecology

      Volume: 101 (10) Issue: 10

    • DOI

      10.1002/ecy.3127

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Adaptive Divergence in Populations of the Stream Mayfly Ephemera strigata with Machine Learning2020

    • Author(s)
      Li, B., S. Yaegashi, T. M. Carvajal, M. Gamboa and K. Watanabe
    • Journal Title

      Ecology and Evolution

      Volume: 10 (13) Issue: 13 Pages: 6677-6687

    • DOI

      10.1002/ece3.6398

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Remarkable New Genus and Species of Nemourinae (Plecoptera, Nemouridae) from Sichuan, China, with Systematic Notes on the Related Genera2020

    • Author(s)
      Mo, R-R., M Gamboa, K. Watanabe, G-Q Wang, W-H Li, D Yang, D Mur?nyi
    • Journal Title

      PLoS ONE

      Volume: 15(3) Issue: 3 Pages: e0229120-e0229120

    • DOI

      10.1371/journal.pone.0229120

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A New Species of Protonemura Kempny, 1898 (Plecoptera: Nemouridae) from the Ishizuchi Range, Shikoku, Japan2020

    • Author(s)
      Mur?nyi, D., M. Gamboa, and K. Watanabe
    • Journal Title

      Zootaxa

      Volume: 4718 (1) Issue: 1 Pages: 57-66

    • DOI

      10.11646/zootaxa.4718.1.4

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Haplotype-level DNA metabarcoding from freshwater macroinvertebrate community samples2021

    • Author(s)
      Joeselle M. Serrana and Kozo Watanabe
    • Organizer
      1st DNAQUA International Conference: Biotic Indices & Metrics - New trends in bioassessment of aquatic ecosystems: from organisms to DNA-based metrics
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insect2021

    • Author(s)
      Kolcsar L.P., Kato D. Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contrasting Adaptive Genetic Consequences of Stream Insects under Changing Climate2021

    • Author(s)
      Nukazawa K., Chiu M-C., Kazama S., Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elevational Patterns of Genetic Diversity in Riverine Populations in Japan2021

    • Author(s)
      Chiu M. C, Watanabe K
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insects2021

    • Author(s)
      Kolcsar L.-P, Kato D., Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Genetic Approaches to the Assessment of Dam Impact on Stream Macroinvertebrates and Dengue Virus Surveillance of Vector Mosquitoes2020

    • Author(s)
      Kozo Watanabe
    • Organizer
      47th Philippine Society of Biochemistry and Molecular Biology (PSBMB) Annual Convention
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16s rDNA amplicon sequencing2020

    • Author(s)
      Joeselle M. Serrana and Kozo Watanabe
    • Organizer
      Joint Meeting of the 3rd Annual Meeting of The eDNA Society and the 36th Annual Meeting of the Society of Population Ecology (Online)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Recurrent seasonal dynamics of the planktonic eukaryotic communities in the polymictic eutrophic Lake M?ggelsee (Germany).2020

    • Author(s)
      Joeselle M. Serrana, Kingsly Chuo Beng, Jusytna Wolinska, Kozo Watanabe and Michael T. Monaghan
    • Organizer
      Water and Environment Technology Conference 2020 Online (WET2020-Online)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Approaches to the Study of Freshwater Biodiversity: Genomic Adaptation and DNA Metabarcoding.2019

    • Author(s)
      Kozo Watanabe
    • Organizer
      International Workshop of Water Quality Monitoring
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Landscape shape predicts enclosed genetic variation within the metapopulation of a species with varied dispersal2019

    • Author(s)
      Ming-Chih Chiu, Kozo Watanabe
    • Organizer
      International Workshop of Water Quality Monitoring
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2019-07-04   Modified: 2022-01-27  

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