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Clarification of mechanism of iron plaque formation in Phragmites australis via microbial siderophores

Research Project

Project/Area Number 19K22151
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 31:Nuclear engineering, earth resources engineering, energy engineering, and related fields
Research InstitutionUniversity of Tsukuba

Principal Investigator

YAMAJI Keiko  筑波大学, 生命環境系, 教授 (00420076)

Co-Investigator(Kenkyū-buntansha) 田中 万也  国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 原子力科学研究所 先端基礎研究センター, 研究主幹 (60377992)
香西 直文  国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 原子力科学研究所 先端基礎研究センター, 研究主席 (80354877)
Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsヨシ / 鉄プラーク / 内生細菌 / 重金属蓄積 / 重金属 / 鉱山 / 自生植物 / 土着微生物
Outline of Research at the Start

水質浄化に関わる水生植物のヨシには、ウランや重金属を蓄積することが判明している。ヨシの浄化機能を高めるためには、植物の元素蓄積機構において寄与度が高い微生物との複合共生系を考慮する必要がある。これまでの研究によって、根の細菌がつくる化合物によって、ヨシにおける重金属吸着能力が増強される可能性が示唆されているが、本研究ではそのメカニズムを解明する。

Outline of Final Research Achievements

Phragmites australis, a wetland plant native to uranium mine tailings sites, developed iron plaques around their roots and accumulated Fe, manganese, and uranium. Many typical siderophores produced by bacteria have a catechol skeleton, but compounds with a catechol skeleton are known to precipitate with Fe. Considering the possibility that endophytic bacterial siderophores may be involved in the formation of Fe plaques, we confirmed the siderophore-producing ability of endophytic bacteria and found that 742 strains, or 88.6% of the 837 strains isolated, had the ability to produce siderophores. It was also found that bacterial metabolites precipitated with Fe, and phenolic compounds thought to be siderophores were also detected.

Academic Significance and Societal Importance of the Research Achievements

鉱山跡地で生育する植物と微生物の複合共生系における元素吸収機構を総合的に解明する研究事例は、国内においてはほとんどなく国際的にも決して多いとは言えない。ヨシは水質浄化能力が高い植物として知られるが、根に形成される鉄プラークが重要な役割を果たしていることが知られている。実際の現場に生育するヨシの根に生息する内生細菌がシデロフォアを産生し、鉄プラークの形成に関与する可能性を示した本成果は、実際の現場における植物を用いた浄化処理技術の発展に利用できると考える。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (16 results)

All 2024 2023 2022 2021 2020 2019

All Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Microbially formed Mn(IV) oxide as a novel adsorbent for removal of Radium2024

    • Author(s)
      Tanaka Kazuya、Yamaji Keiko、Masuya Hayato、Tomita Jumpei、Ozawa Mayumi、Yamasaki Shinya、Tokunaga Kohei、Fukuyama Kenjin、Ohara Yoshiyuki、Maamoun Ibrahim、Yamaguchi Akiko、Takahashi Yoshio、Kozai Naofumi、Grambow Bernd
    • Journal Title

      Chemosphere

      Volume: 355 Pages: 141837-141837

    • DOI

      10.1016/j.chemosphere.2024.141837

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isoavenaciol and 7-hydroxy-isoavenaciol: Zn-chelating metallophores produced by root-endophytic Pezicula ericae in a Zn-accumulating plant, Aucuba japonica2023

    • Author(s)
      Doyama Kohei、Haruma Toshikatsu、Hishiyama Shojiro、Kato Atsushi、Masuya Hayato、Yamaji Keiko
    • Journal Title

      Phytochemistry

      Volume: 206 Pages: 113547-113547

    • DOI

      10.1016/j.phytochem.2022.113547

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fe, Mn and 238U Accumulations in Phragmites australis Naturally Growing at the Mill Tailings Pond; Iron Plaque Formation Possibly Related to Root-Endophytic Bacteria Producing Siderophores2021

    • Author(s)
      Nakamoto Yukihiro、Doyama Kohei、Haruma Toshikatsu、Lu Xingyan、Tanaka Kazuya、Kozai Naofumi、Fukuyama Kenjin、Fukushima Shigeru、Ohara Yoshiyuki、Yamaji Keiko
    • Journal Title

      Minerals

      Volume: 11 Issue: 12 Pages: 1337-1337

    • DOI

      10.3390/min11121337

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preferential elimination of Ba2+ through irreversible biogenic oxide sequestration2021

    • Author(s)
      Y. Tani, S. Kakinuma, J. Chang, K. Tanaka, and N. Miyata
    • Journal Title

      Minerals

      Volume: 11 Issue: 1 Pages: 53-53

    • DOI

      10.3390/min11010053

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Study on coordination structure of Re adsorbed on Mg-Al layered double hydroxide using X-ray absorption fine structure.2019

    • Author(s)
      Tanaka, K., Kozai, N., Ohnuki, T. and Grambow, B.
    • Journal Title

      Journal of Porous Materials

      Volume: 26 Issue: 2 Pages: 505-511

    • DOI

      10.1007/s10934-018-0634-z

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Adsorption mechanism of ReO4- on Ni-Zn layered hydroxide salt and its application to removal of ReO4- as a surrogate of TcO4-.2019

    • Author(s)
      Tanaka, K., Kozai, N., Yamasaki, Y., Ohnuki, T., Kaplan, D.I. and Grambow, B.
    • Journal Title

      Applied Clay Science

      Volume: 182 Pages: 1-8

    • DOI

      10.1016/j.clay.2019.105282

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 鉱さいたい積場のヨシの重金属耐性と重金属蓄積に関与する内生糸状菌の化学的機能2024

    • Author(s)
      大熊美結 盧星燕 谷内美月 中本幸弘 土肥輝美 香西直文 福山賢仁 綱嶋康倫 升屋勇人 坂口綾 山路恵子
    • Organizer
      日本生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Elucidation of Heavy-metal Tolerance Mechanism in Phragmites australis (Cav.) Trin. ex Steud. without Iron Plaque Formation2022

    • Author(s)
      Miyu Okuma, Keiko Yamaji, Yukihiro Nakamoto, Kenjin Fukuyama, Yasumichi Tsunashima
    • Organizer
      INTECOL 2022 Frontiers in Ecology: Science & Society
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Heavy-Metal Accumulations in Phragmites australis Naturally Growing at Mill Tailings Pond; Iron Plaque Formation Related to Siderophores by Root-endophytic Bacteria2022

    • Author(s)
      Keiko Yamaji, Yukihiro Nakamoto, Kohei Doyama, Toshikatsu Haruma, Xingyan Lu, Kazuya Tanaka, Naofumi Kozai, Kenjin Fukuyama K4, Fukushima, and Yoshiyuki Ohara
    • Organizer
      INTECOL 2022 Frontiers in Ecology: Science & Society
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 鉱さいたい積場のヨシにおける重金属元素の蓄積及び鉄プラーク形成へ関与する内生細菌2022

    • Author(s)
      中本幸弘, 山路恵子, 春間俊克, 土山紘平, 盧星燕, 田中万也, 香西直文, 福山賢仁, 福嶋繁, 小原義之
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヨシの根におけるウラン吸着能および鉄プラーク形成機構の解明2022

    • Author(s)
      春間俊克, 土山紘平, 田中万也, 高橋嘉夫, 福山賢仁, 小原義之, 山路 恵子
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヨシ(Phragmites australis)におけるウラン及び重金属(Fe, Mn)蓄積への内生細菌の関与2021

    • Author(s)
      中本幸弘, 山路恵子, 春間俊克, 土山紘平, 小原義之, 福山賢仁, 福嶋繁
    • Organizer
      日本生態学会第68回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 鉱山跡地の自生植物と機能性微生物の相互作用の解明2020

    • Author(s)
      山路恵子・春間俊克・中本幸弘・土山紘平・盧星燕・野路建太・升屋隼人・田中万也・田村憲司・小川 和義・森 茂太・黒澤 陽子・香西 直文
    • Organizer
      第71回コロイドおよび界面化学討論会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Root endophytic Chaetomium cupreum increased Al tolerance in Miscanthus sinensis growing at an old mine site.2019

    • Author(s)
      Toshikatsu Haruma,Keiko Yamaji,Kazuyoshi Ogawa,Hayato Masuya,Yurina Sekine,Naofumi Kozai
    • Organizer
      Asian Mycology Congress 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 内生菌が促進する、鉱山跡地におけるススキの定着2019

    • Author(s)
      春間俊克,山路恵子
    • Organizer
      資源・素材学会平成31 (2019) 年度春季大会.
    • Related Report
      2019 Research-status Report
  • [Presentation] 鉱山跡地において,内生菌が関与するススキの有害金属耐性機構の解明2019

    • Author(s)
      春間俊克, 山路恵子, 盧星燕, 土山紘平
    • Organizer
      資源・素材2019(京都)2019年度資源・素材関係学協合同秋季大会
    • Related Report
      2019 Research-status Report

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Published: 2019-07-04   Modified: 2025-01-30  

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